Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 499,328 C→T 13.9% V306I (GTT→ATT)  modF ← fused subunits of molybdate transporter and ATP‑binding components of ABC superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE499,3280CT13.9% 218.9 / 24.0 86V306I (GTT→ATT) modFfused subunits of molybdate transporter and ATP‑binding components of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base C (29/45);  new base T (7/5);  total (36/50)
Fisher's exact test for biased strand distribution p-value = 2.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 104 total aligned reads displayed.

CGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTC  >  minE/499273‑499395
                                                       |                                                                   
cgtcgtCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAATCAGGCTTAATAACGt                                                      <  1:679665/71‑1 (MQ=255)
cgtcgtCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAATCAGGCTTAATAACGt                                                      <  1:3802937/71‑1 (MQ=255)
cgtcgtCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAATCAGGCTTAATAACGt                                                      <  1:2101250/71‑1 (MQ=255)
cgtcgtCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAATCAGGCTTAATAACGt                                                      <  1:2832912/71‑1 (MQ=255)
  tcgtcCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTtaat                                                           <  1:3510011/64‑1 (MQ=255)
  tcgtcCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTtaat                                                           <  1:3445157/64‑1 (MQ=255)
  tcgtcCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTtaat                                                           <  1:3110641/64‑1 (MQ=255)
  tcgtcCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTtaat                                                           <  1:2473908/64‑1 (MQ=255)
    gtacGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGAt                                                  >  1:3888713/4‑71 (MQ=255)
     tcCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAAc                                                                     <  1:3097193/51‑1 (MQ=255)
     tcCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAAc                                                                     <  1:2567415/51‑1 (MQ=255)
     tcCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAAc                                                                     <  1:2640659/51‑1 (MQ=255)
     tcCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAAc                                                                     <  1:5040941/51‑1 (MQ=255)
     tcCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAAc                                                                     <  1:2813463/51‑1 (MQ=255)
     tcCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAAc                                                                     <  1:1982529/51‑1 (MQ=255)
     tcCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAAc                                                                     <  1:234397/51‑1 (MQ=255)
     tcCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAAc                                                                     <  1:4206634/51‑1 (MQ=255)
     tcCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAAc                                                                     <  1:1735845/51‑1 (MQ=255)
     tcCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAAc                                                                     <  1:1624566/51‑1 (MQ=255)
     tcCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAAc                                                                     <  1:824318/51‑1 (MQ=255)
     tcCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAAc                                                                     <  1:1379515/51‑1 (MQ=255)
     tcCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAAc                                                                     <  1:3593591/51‑1 (MQ=255)
     tcCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAAc                                                                     <  1:3977486/51‑1 (MQ=255)
       cGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCa                                                                   >  1:40767/1‑51 (MQ=255)
       cGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCa                                                                   >  1:2430712/1‑51 (MQ=255)
       cGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCa                                                                   >  1:2747596/1‑51 (MQ=255)
       cGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCa                                                                   >  1:4269749/1‑51 (MQ=255)
       cGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCa                                                                   >  1:3692090/1‑51 (MQ=255)
       cGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCa                                                                   >  1:3686641/1‑51 (MQ=255)
       cGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCa                                                                   >  1:1551930/1‑51 (MQ=255)
       cGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCa                                                                   >  1:2293337/1‑51 (MQ=255)
       cGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCa                                                                   >  1:2126833/1‑51 (MQ=255)
       cGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAg                                                                  <  1:4375662/52‑1 (MQ=255)
       cGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAg                                                                  <  1:5145137/52‑1 (MQ=255)
       cGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAg                                                                  <  1:887540/52‑1 (MQ=255)
       cGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGAt                                                  <  1:4069159/68‑1 (MQ=255)
       cGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGAt                                                  <  1:3105254/68‑1 (MQ=255)
       cGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGAt                                                  <  1:934909/68‑1 (MQ=255)
        gAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTc                                              >  1:2593914/1‑71 (MQ=255)
         aaaaGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAAc                                                                     >  1:3914315/1‑47 (MQ=255)
         aaaaGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAAc                                                                     >  1:4033638/1‑47 (MQ=255)
         aaaaGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAAc                                                                     >  1:728538/1‑47 (MQ=255)
         aaaaGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAAc                                                                     >  1:1714713/1‑47 (MQ=255)
           aaGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTg                                           >  1:3471717/1‑71 (MQ=255)
           aaGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTg                                           >  1:2579915/1‑71 (MQ=255)
             gCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCa                                         >  1:1450767/1‑71 (MQ=255)
             gCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCa                                         >  1:1702847/1‑71 (MQ=255)
               gTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCAcc                                       <  1:1880869/71‑1 (MQ=255)
               gTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCAcc                                       <  1:1711513/71‑1 (MQ=255)
                tCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCa                                      <  1:2990954/71‑1 (MQ=255)
                tCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCa                                      <  1:4556976/71‑1 (MQ=255)
                tCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCa                                      <  1:2222241/71‑1 (MQ=255)
                tCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCa                                      <  1:2635568/71‑1 (MQ=255)
                  aaaTCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCAtt                                    >  1:3481011/1‑71 (MQ=255)
                     tCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTgg                                 >  1:1934164/1‑71 (MQ=255)
                     tCGTGGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTgg                                 >  1:4659727/1‑71 (MQ=255)
                      cGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTtaat                                                           <  1:3093350/44‑1 (MQ=255)
                      cGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTtaat                                                           <  1:1581912/44‑1 (MQ=255)
                      cGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTtaat                                                           <  1:3335277/44‑1 (MQ=255)
                      cGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTtaat                                                           <  1:3271849/44‑1 (MQ=255)
                      cGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTtaat                                                           <  1:728985/44‑1 (MQ=255)
                      cGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTtaat                                                           <  1:2858079/44‑1 (MQ=255)
                      cGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTtaat                                                           <  1:4660149/44‑1 (MQ=255)
                        ttGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTtaataa                                                         >  1:3914879/1‑44 (MQ=255)
                        ttGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTtaataa                                                         >  1:3531107/1‑44 (MQ=255)
                        ttGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTtaataa                                                         >  1:102136/1‑44 (MQ=255)
                          gCTGTAACCTTGCGGATGATCGCCAGTAATCAGGCTTAATAACGt                                                      <  1:3370513/45‑1 (MQ=255)
                          gCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGa                            >  1:942090/1‑71 (MQ=255)
                           cTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGAc                           <  1:1027847/71‑1 (MQ=255)
                             gTAACCTTGCGGATGATCGCCAGTAATCAGGCTTAATAACGTCGATTTTcccg                                           >  1:3258058/1‑51 (MQ=39)
                             gTAACCTTGCGGATGATCGCCAGTAATCAGGCTTAATAACGTCGATTTTCCCGCGCCATTTGGCCCGACaa                         >  1:502067/1‑71 (MQ=255)
                             gTAACCTTGCGGATGATCGCCAGTAATCAGGCTTAATAACGTCGATTTTCCCGCGCCATTTGGCCCGACaa                         >  1:3660575/1‑71 (MQ=255)
                             gTAACCTTGCGGATGATCGCCAGTAATCAGGCTTAATAACGTCGATTTTCCCGCGCCATTTGGCCCGACaa                         >  1:1905751/1‑71 (MQ=255)
                             gTAACCTTGCGGATGATCGCCAGTAATCAGGCTTAATAACGTCGATTTTCCCGCGCCATTTGGCCCGACaa                         >  1:2421020/1‑71 (MQ=255)
                             gTAACCTTGCGGATGATCGCCAGTAATCAGGCTTAATAACGTCGATTTTCCCGCGCCATTTGGCCCGACaa                         >  1:4336819/1‑71 (MQ=255)
                             gTAACCTTGCGGATGATCGCCAGTAATCAGGCTTAATAACGTCGATTTTCCCGCGCCATTTGGCCCGACaa                         >  1:2895353/1‑71 (MQ=255)
                              tAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACa                          <  1:5052646/69‑1 (MQ=255)
                              tAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACa                          <  1:1947107/69‑1 (MQ=255)
                              tAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACa                          <  1:4007762/69‑1 (MQ=255)
                               aaCCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCa                                         >  1:1427975/1‑53 (MQ=255)
                               aaCCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAAtt                       >  1:4563646/1‑71 (MQ=255)
                                aCCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAAttt                      >  1:325960/1‑71 (MQ=255)
                                     gCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGcc                   <  1:4623285/69‑1 (MQ=255)
                                     gCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGcc                   <  1:709344/69‑1 (MQ=255)
                                     gCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGcc                   <  1:4851594/69‑1 (MQ=255)
                                     gCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGcc                   <  1:1120504/69‑1 (MQ=255)
                                     gCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGcc                   <  1:2925106/69‑1 (MQ=255)
                                      cGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAg                 <  1:2167585/70‑1 (MQ=255)
                                       ggATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAgtg               <  1:2283327/71‑1 (MQ=255)
                                        gatgatCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAgtgt              <  1:4692564/71‑1 (MQ=255)
                                        gatgatCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAgtgt              <  1:2624338/71‑1 (MQ=255)
                                        gatgatCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAgtgt              <  1:2332022/71‑1 (MQ=255)
                                                 cAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGa     <  1:481792/71‑1 (MQ=255)
                                                   gTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGAtt   >  1:4907040/1‑71 (MQ=255)
                                                   gTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGAtt   <  1:1000571/71‑1 (MQ=255)
                                                   gTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGAt    >  1:2828815/1‑70 (MQ=255)
                                                    tAACCATGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTc  <  1:2392919/71‑1 (MQ=255)
                                                    tAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTc  <  1:4874918/71‑1 (MQ=255)
                                                    tAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTc  <  1:239576/71‑1 (MQ=255)
                                                    tAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTc  <  1:4522598/71‑1 (MQ=255)
                                                       |                                                                   
CGTCGTCCGAAAAGCGTCAAATCGTTGCTGTAACCTTGCGGATGATCGCCAGTAACCAGGCTTAATAACGTCGATTTTCCTGCACCATTTGGCCCGACAATTTGCCAGTGTTCGCCTGGATTC  >  minE/499273‑499395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: