| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 550,934 | T→C | 9.1% | K4K (AAA→AAG) | ybiV ← | predicted hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 550,934 | 0 | T | C | 9.1% | 218.7 / 10.4 | 77 | K4K (AAA→AAG) | ybiV | predicted hydrolase |
| Reads supporting (aligned to +/- strand): ref base T (33/37); new base C (2/5); total (35/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01 | |||||||||||
ATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAGTGTAATAACAATGTGA > minE/550866‑550997 | aTAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCGTCCATGTCTGTGACGATAACttt < 1:4236989/71‑1 (MQ=255)aTAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCGTCCATGTCTGTGACGATAACttt < 1:4415545/71‑1 (MQ=255)aTAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCGTCCATGTCTGTGACGATAACttt < 1:4845396/71‑1 (MQ=255) tAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCGTCCATGTCTGTGACGATAACttt > 1:2973472/1‑70 (MQ=255) aaaCGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTa > 1:2230703/1‑70 (MQ=255) aaaCGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTAc > 1:2761889/1‑71 (MQ=255) ggttggttGTACGTTTTGGCGTCGTTAAGAAAAGTACCGTCCATGTCTGTGACGATAACttt < 1:1543378/62‑1 (MQ=255) aCGTTTTGGCGTCGTTAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTc > 1:128666/1‑71 (MQ=255) gTTTTGGCGTCGTTAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCt > 1:3742891/1‑71 (MQ=255) gTTTTGGCGTCGTTAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCt > 1:5190347/1‑71 (MQ=255) ttGGCGTCGTTAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTa > 1:4984247/1‑49 (MQ=255) ttGGCGTCGTTAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTa > 1:4632488/1‑49 (MQ=255) ttGGCGTCGTTAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTa > 1:4946738/1‑49 (MQ=255) ttGGCGTCGTTAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTa > 1:1879992/1‑49 (MQ=255) ttGGCGTCGTTAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGc > 1:2404106/1‑71 (MQ=255) gCGTCGTTAAGAAAAGTACCGTCCATGTCTGTGACGATAACCTTTACGCt < 1:70340/50‑1 (MQ=255) gCGTCGTTAAGAAAAGTACCGTCCATGTCTGTGACGATAACCTTTACGCt < 1:3761266/50‑1 (MQ=255) gCGTCGTTAAGAAAAGTACCGTCCATGTCTGTGACGATAACCTTTACGCt < 1:2873868/50‑1 (MQ=255) gCGTCGTTAAGAAAAGTACCGTCCATGTCTGTGACGATAACCTTTACGCt < 1:765400/50‑1 (MQ=255) gCGTCGTTAAGAAAAGTACCGTCCATGTCTGTGACGATAACCTTTACGCt < 1:846474/50‑1 (MQ=255) gtcgtTAAGAAAAGTACCGTCCATGTCTGTGACGATAACCTTTACGCTCa > 1:4047421/1‑50 (MQ=255) gtcgtTAAGAAAAGTACCGTCCATGTCTGTGACGATAACCTTTACGCTCa > 1:427499/1‑50 (MQ=255) tcgtTAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTACGCTCATAgg < 1:1514052/53‑1 (MQ=255) tcgtTAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTACGCTCATAgg < 1:913198/53‑1 (MQ=255) tcgtTAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTACGCTCATAgg < 1:1299078/53‑1 (MQ=255) tcgtTAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTACGCTCATAgg < 1:2774294/53‑1 (MQ=255) tcgtTAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTACGCTCATAgg < 1:2088142/53‑1 (MQ=255) tcgtTAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTACGCTCATAgg < 1:2425023/53‑1 (MQ=255) tcgtTAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTACGCTCATAgg < 1:2982195/53‑1 (MQ=255) tcgtTAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGaaa < 1:2977197/71‑1 (MQ=255) cgtTAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTACGCTCATAGGTTTGCTCCTGGCTCGaaaa > 1:107857/1‑71 (MQ=255) gtTAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTACGCTCATAgg < 1:4665260/51‑1 (MQ=255) gtTAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAAt > 1:1747415/1‑71 (MQ=255) ttAATAAAAGTACCGTCCATGTCTGTGACGATAACTTTTACGCTCAt < 1:683418/47‑1 (MQ=255) ttAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTACGCTCAt < 1:4158328/47‑1 (MQ=255) ttAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTACGCTCAt < 1:3923280/47‑1 (MQ=255) ttAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTACGCTCAt < 1:956968/47‑1 (MQ=255) ttAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTACGCTCAt < 1:3674537/47‑1 (MQ=255) aaGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTACGCTCa > 1:636195/1‑44 (MQ=255) aaGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTACGCTCa > 1:395323/1‑44 (MQ=255) tACCGTCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAAc < 1:3820587/71‑1 (MQ=255) ccGTCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAg > 1:3973948/1‑71 (MQ=255) ccGTCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAg > 1:5163351/1‑71 (MQ=255) ccGTCCATGCCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAg > 1:3389272/1‑71 (MQ=255) tCCATGTCTGTGACGATAACTTTTACGCTCATAgg < 1:5172810/35‑1 (MQ=255) ccATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTc < 1:2632372/41‑1 (MQ=255) cATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGaaa > 1:3097496/1‑57 (MQ=255) cATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGaaa > 1:5095454/1‑57 (MQ=255) cATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGaaa > 1:2945563/1‑57 (MQ=255) cATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGaaa > 1:2119032/1‑57 (MQ=255) cATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGaaa > 1:2068874/1‑57 (MQ=255) cATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGaaa > 1:4586046/1‑57 (MQ=255) cATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAGTGtaa > 1:81579/1‑71 (MQ=255) cATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAGTGtaa > 1:46324/1‑71 (MQ=255) aTGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAAc < 1:1171226/63‑1 (MQ=255) tCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAGTGtaataa > 1:762443/1‑70 (MQ=255) tCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAGTGtaataa > 1:3642527/1‑70 (MQ=255) tCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAGTGTAATAAc < 1:2686864/71‑1 (MQ=255) tCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAGTGTAATAAc < 1:4854070/71‑1 (MQ=255) tCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAGTGTAATAAc < 1:2164194/71‑1 (MQ=255) tgACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGa > 1:1508038/1‑47 (MQ=255) tgACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGa > 1:792570/1‑47 (MQ=255) tgACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAgtg < 1:3584167/60‑1 (MQ=255) tgACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAgtg < 1:103121/60‑1 (MQ=255) tgACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAgtg < 1:5017026/60‑1 (MQ=255) tgACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAgtg < 1:231315/60‑1 (MQ=255) tgACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAgtg < 1:4723932/60‑1 (MQ=255) tgACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAgtg < 1:3167098/60‑1 (MQ=255) tgACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAgtg < 1:4619111/60‑1 (MQ=255) tgACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAgtg < 1:2252070/60‑1 (MQ=255) tgACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAGTGTAATAACAAtg < 1:3288939/71‑1 (MQ=255) aCGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAGTGTAATAACAAtgt < 1:2505390/70‑1 (MQ=255) aTAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGaaa < 1:2840765/38‑1 (MQ=255) aTAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGaaa < 1:1382643/38‑1 (MQ=255) aTAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGaaa < 1:300992/38‑1 (MQ=255) aTAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGaaa < 1:2891208/38‑1 (MQ=255) aTAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGaaa < 1:1827974/38‑1 (MQ=255) aTAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGaaa < 1:525170/38‑1 (MQ=255) aTAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAGTGTAATAACAATGTGa > 1:4179569/1‑69 (MQ=255) aTAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAGTGTAATAACAATGTGa > 1:2599813/1‑69 (MQ=255) aTAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAGTGTAATAACAATGTGa > 1:4693659/1‑69 (MQ=255) aTAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAGTGTAATAACAATGTGa > 1:3599391/1‑69 (MQ=255) | ATAAAACGTGGTTGGTTGTACGTTTTGGCGTCGTTAAGAAAAGTACCGTCCATGTCTGTGACGATAACTTTTACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAGTGTAATAACAATGTGA > minE/550866‑550997 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |