| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 710,036 | G→C | 17.3% | intergenic (‑162/+99) | yceJ ← / ← yceO | predicted cytochrome b561/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 710,036 | 0 | G | C | 17.3% | 122.3 / 18.6 | 52 | intergenic (‑162/+99) | yceJ/yceO | predicted cytochrome b561/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (20/23); new base C (4/5); total (24/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
TTCAATAATTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAA > minE/709972‑710105 | ttCAATAATTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCt < 1:2006252/68‑1 (MQ=255) tCAATAATTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCt < 1:1880417/67‑1 (MQ=255) aataatTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGa > 1:2126073/1‑70 (MQ=255) aataatTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAg > 1:2796381/1‑71 (MQ=255) atTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGc < 1:5112651/70‑1 (MQ=255) atTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCt < 1:4283841/71‑1 (MQ=255) ttAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGt < 1:2782867/65‑1 (MQ=255) ttAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGt < 1:3374947/65‑1 (MQ=255) ataatGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAgg < 1:3901896/71‑1 (MQ=255) ataatGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAgg < 1:2612838/71‑1 (MQ=255) ataatGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAgg < 1:2925708/71‑1 (MQ=255) gAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGaataa > 1:1986808/1‑71 (MQ=255) aaaGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGaata > 1:4771741/1‑69 (MQ=255) aGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGt < 1:4497772/45‑1 (MQ=255) aGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGt < 1:3233958/45‑1 (MQ=255) aaTCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGaa < 1:1031990/63‑1 (MQ=255) ttCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGt < 1:212990/48‑1 (MQ=255) aTAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAtt > 1:3057822/1‑71 (MQ=255) aTAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAtt < 1:2517586/71‑1 (MQ=255) aTAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAtt > 1:1948864/1‑71 (MQ=255) aaGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAgg < 1:3654907/52‑1 (MQ=255) aaGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAtttt > 1:4267481/1‑71 (MQ=255) aGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAttttt > 1:3919562/1‑71 (MQ=255) aGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAttttt > 1:2999409/1‑71 (MQ=255) aGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAttttt < 1:1171786/71‑1 (MQ=255) aGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAtttt > 1:4025461/1‑70 (MQ=255) tttAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCa < 1:4179995/71‑1 (MQ=255) tttAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCa < 1:1035620/71‑1 (MQ=255) tttAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCa < 1:2357741/71‑1 (MQ=255) tttAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCa < 1:1497317/71‑1 (MQ=255) tCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAt > 1:3048406/1‑55 (MQ=255) tCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAt > 1:3478854/1‑55 (MQ=255) tCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAt > 1:3607620/1‑55 (MQ=255) tCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAt > 1:5026125/1‑55 (MQ=255) aGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTACATGaaa < 1:672418/71‑1 (MQ=255) aGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGaaa < 1:999210/71‑1 (MQ=255) aGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGaaa < 1:4633094/71‑1 (MQ=255) cATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAgg > 1:4060223/1‑71 (MQ=255) accAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAgg > 1:3190029/3‑70 (MQ=255) accAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAgg > 1:3009245/3‑70 (MQ=255) accAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAgg > 1:2914268/3‑70 (MQ=255) accAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAg > 1:2008248/3‑69 (MQ=255) tCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAgg > 1:4128032/1‑69 (MQ=255) ccAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGga < 1:4337496/70‑1 (MQ=255) ccAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGga < 1:4623873/70‑1 (MQ=255) ccAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGga < 1:1123524/70‑1 (MQ=255) ccAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTACACGCTAAATGAAAGGga < 1:4253674/70‑1 (MQ=255) ccAGACATCTCTGATCAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGga < 1:4721577/70‑1 (MQ=255) agagATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAgg > 1:1713701/1‑67 (MQ=255) agagATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAgg > 1:17136/1‑67 (MQ=255) gaTGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACg > 1:1345833/1‑52 (MQ=255) gaTGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACg > 1:519359/1‑52 (MQ=255) gTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCTCGCTAAATGAAAGGGAGATTaaa > 1:4769643/1‑70 (MQ=255) gTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTaaa > 1:1521298/1‑70 (MQ=255) gTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTaaa > 1:1289129/1‑70 (MQ=255) | TTCAATAATTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAA > minE/709972‑710105 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |