| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 949,284 | T→A | 5.1% | Y518F (TAT→TTT) | dos ← | cAMP phosphodiesterase, heme‑regulated |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 949,284 | 0 | T | A | 5.1% | 531.9 / 14.6 | 177 | Y518F (TAT→TTT) | dos | cAMP phosphodiesterase, heme‑regulated |
| Reads supporting (aligned to +/- strand): ref base T (83/85); new base A (7/2); total (90/87) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.59e-01 | |||||||||||
Only 100 of 183 total aligned reads displayed.
TTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCC > minE/949220‑949351 | ttttACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATc < 1:4853207/69‑1 (MQ=255)ttttACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCca > 1:2404257/1‑71 (MQ=255) ttACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAAt > 1:4494184/1‑66 (MQ=255) ttCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATtgt > 1:1304276/1‑71 (MQ=255) ttCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATtgt > 1:1889258/1‑71 (MQ=255) tCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCcattgcatt > 1:3801805/1‑68 (MQ=255) tCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCcattgcatt > 1:3259066/1‑68 (MQ=255) tCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCcattgcatt > 1:3921946/1‑68 (MQ=255) tCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATtgt < 1:4013923/70‑1 (MQ=255) tCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATtgt < 1:2610093/70‑1 (MQ=255) cGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATtgt < 1:4059801/69‑1 (MQ=255) tCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTg < 1:2204591/71‑1 (MQ=255) cGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGt > 1:4447356/1‑69 (MQ=255) cGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTg > 1:3428763/1‑70 (MQ=255) cGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTg > 1:3647636/1‑70 (MQ=255) gCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCca > 1:907889/1‑52 (MQ=255) gCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTg > 1:3172090/1‑69 (MQ=255) gCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTg > 1:195857/1‑69 (MQ=255) gCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGga < 1:832786/71‑1 (MQ=255) cGGGCTGAAGAACTGCCAGCCGTTACCGCCATTTTTGCGAATAAAATcc > 1:238329/1‑49 (MQ=255) gggCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGagag > 1:1837520/1‑71 (MQ=255) gggCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGagag > 1:2154142/1‑71 (MQ=255) gggCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGagag > 1:3543590/1‑71 (MQ=255) ggCTGAAGAACTGCCAGCCGTTACCGCCATTTTTGCGAATAAAATCCATTGCATTGTGAGCAGTGGAGAGc < 1:3418388/71‑1 (MQ=255) ggCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTg < 1:3951963/65‑1 (MQ=255) ggCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTg < 1:3426810/65‑1 (MQ=255) ggCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGc < 1:1665404/71‑1 (MQ=255) cTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCcattt < 1:4188905/49‑2 (MQ=255) cTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCcattg < 1:1379516/49‑1 (MQ=255) cTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCcattg < 1:3221560/49‑1 (MQ=255) cTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCcattg < 1:2110009/49‑1 (MQ=255) aagaaCTGCCAGCCGTTACCGCCATTCTTGCGAAtata > 1:1688614/1‑38 (MQ=255) aagaaCTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCca > 1:1075228/1‑43 (MQ=255) aagaaCTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCca > 1:1269457/1‑43 (MQ=255) aagaaCTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTa < 1:1390422/71‑1 (MQ=255) aagaaCTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTa < 1:3305071/71‑1 (MQ=255) aCTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCcat > 1:3784309/1‑40 (MQ=255) aCTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAg > 1:1476048/1‑51 (MQ=255) aCTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAg > 1:94200/1‑51 (MQ=255) aCTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATcg < 1:3082458/71‑1 (MQ=255) aCTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATcg < 1:491823/71‑1 (MQ=255) aCTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATcg < 1:4043950/71‑1 (MQ=255) aCTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATcg < 1:2519010/71‑1 (MQ=255) aCTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATcg < 1:1811619/71‑1 (MQ=255) aCTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATcg < 1:806614/71‑1 (MQ=255) cTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATcgc < 1:99338/71‑1 (MQ=255) cTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATcgc < 1:2016355/71‑1 (MQ=255) cTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATcgc < 1:2022420/71‑1 (MQ=255) cTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATcgc < 1:4969797/71‑1 (MQ=255) tGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGgaga < 1:632008/58‑1 (MQ=255) tGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGgaga < 1:3123118/58‑1 (MQ=255) tGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGgaga < 1:3111264/58‑1 (MQ=255) ccAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGt < 1:1161079/71‑1 (MQ=255) ccAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGt < 1:4568043/71‑1 (MQ=255) gCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTg < 1:4024905/49‑1 (MQ=255) gCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTg < 1:4689188/49‑1 (MQ=255) gCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTg < 1:4519679/49‑1 (MQ=255) gCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTg < 1:3886360/49‑1 (MQ=255) gCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTg < 1:4675600/49‑1 (MQ=255) gCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTg < 1:1115038/49‑1 (MQ=255) gCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTg < 1:2003048/49‑1 (MQ=255) gCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTg < 1:334784/49‑1 (MQ=255) gCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTg < 1:3164259/49‑1 (MQ=255) gCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGtttt < 1:642695/71‑1 (MQ=255) gCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGtttt < 1:3473689/71‑1 (MQ=255) gCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGtttt < 1:2887414/71‑1 (MQ=255) ccGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGtttt < 1:2830747/70‑1 (MQ=255) cGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTa > 1:3778022/1‑71 (MQ=255) aTTCTTGCGAATATAATCCATTGCATTGTGAGCAg > 1:1807322/1‑35 (MQ=255) aTTCTTGCGAATATAATCCATTGCATTGTGAGCAg > 1:726236/1‑35 (MQ=255) aTTCTTGCGAATATAATCCATTGCATTGTGAGCAg > 1:5150759/1‑35 (MQ=255) aTTCTTGCGAATATAATCCATTGCATTGTGAGCAg > 1:2356825/1‑35 (MQ=255) aTTCTTGCGAATATAATCCATTGCATTGTGAGCAg > 1:482657/1‑35 (MQ=255) aTTCTTGCGAATATAATCCATTGCATTGTGAGCAg > 1:2773818/1‑35 (MQ=255) aTTCTTGCGAATATAATCCATTGCATTGTGAGCAg > 1:2791261/1‑35 (MQ=255) aTTCTTGCGAATATAATCCATTGCATTGTGAGCAg > 1:945000/1‑35 (MQ=255) aTTCTTGCGAATATAATCCATTGCATTGTGAGCAg > 1:3513873/1‑35 (MQ=255) aTTCTTGCGAATATAATCCATTGCATTGTGAGCAg > 1:35478/1‑35 (MQ=255) aTTCTTGCGAATATAATCCATTGCATTGTGAGCAg > 1:4346069/1‑35 (MQ=255) aTTCTTGCGAATATAATCCATTGCATTGTGAGCAg > 1:3615542/1‑35 (MQ=255) aTTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGACGt < 1:5090155/71‑1 (MQ=255) aTTCTTACGAATATAATCCATTGCATTGTGAGCAg > 1:669841/1‑35 (MQ=255) ttCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATcg < 1:4027638/51‑1 (MQ=255) ttCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTa < 1:2718/71‑1 (MQ=255) cTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATcgcg > 1:2051324/1‑51 (MQ=255) cTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATcgcg > 1:4104916/1‑51 (MQ=255) cTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATcgcg > 1:4403292/1‑51 (MQ=255) cTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGtttt > 1:4289661/1‑56 (MQ=255) cTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGc > 1:4862765/1‑71 (MQ=255) cTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGc > 1:2627983/1‑71 (MQ=255) cTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGc > 1:2934958/1‑71 (MQ=255) tGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCt > 1:5059332/1‑70 (MQ=255) gCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCt < 1:1528482/69‑1 (MQ=255) gCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCt < 1:545286/69‑1 (MQ=255) gCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCt < 1:4697918/69‑1 (MQ=255) gCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCt < 1:4361120/69‑1 (MQ=255) atatAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGcc < 1:1420626/71‑1 (MQ=255) tataATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGttttt < 1:310320/49‑1 (MQ=255) tataATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGttttt < 1:3686532/49‑1 (MQ=255) tataATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGt < 1:2273558/45‑1 (MQ=255) | TTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCC > minE/949220‑949351 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |