| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,185,010 | C→T | 10.6% | Y325Y (TAC→TAT) | yeaG → | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,185,010 | 0 | C | T | 10.6% | 347.7 / 24.0 | 122 | Y325Y (TAC→TAT) | yeaG | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base C (41/68); new base T (11/2); total (52/70) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.90e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 | |||||||||||
Only 100 of 126 total aligned reads displayed.
GAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCA > minE/1184944‑1185078 | gAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCg > 1:1649587/1‑71 (MQ=255) cGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa < 1:2610481/71‑1 (MQ=255) cGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa < 1:218075/71‑1 (MQ=255) cGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa < 1:899725/71‑1 (MQ=255) cGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa < 1:3669232/71‑1 (MQ=255) cGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa < 1:5153118/71‑1 (MQ=255) gAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGaa < 1:4863717/71‑1 (MQ=255) gggTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGaa < 1:2938971/61‑1 (MQ=255) tgTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTAtt < 1:3752969/70‑1 (MQ=255) ggTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTAtt < 1:1239636/71‑1 (MQ=255) cTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTAttgcttg < 1:4523425/71‑1 (MQ=255) cTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTAttgcttg < 1:3220125/71‑1 (MQ=255) cTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTAttgcttg < 1:3882260/71‑1 (MQ=255) tttCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa < 1:5108633/53‑1 (MQ=255) tttCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa < 1:2367878/53‑1 (MQ=255) ccGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCa > 1:280797/1‑71 (MQ=255) gTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa < 1:2545427/48‑1 (MQ=255) gTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa < 1:4829017/48‑1 (MQ=255) gTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa < 1:919603/48‑1 (MQ=255) gTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa < 1:2128243/48‑1 (MQ=255) gTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa < 1:4089734/48‑1 (MQ=255) gTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa < 1:4964384/48‑1 (MQ=255) gTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa < 1:3250381/48‑1 (MQ=255) taataaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa < 1:4254376/47‑1 (MQ=255) taataaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGa < 1:4002593/47‑1 (MQ=255) taaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg < 1:1346692/71‑1 (MQ=255) taaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg < 1:2230309/71‑1 (MQ=255) taaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg < 1:2328440/71‑1 (MQ=255) taaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg < 1:4328090/71‑1 (MQ=255) taaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg < 1:1500764/71‑1 (MQ=255) aaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTgcgc < 1:3312983/63‑1 (MQ=255) aaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTgcgc < 1:4457892/63‑1 (MQ=255) aaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTgcgc < 1:3078019/63‑1 (MQ=255) aCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg > 1:106081/1‑69 (MQ=255) aCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg > 1:2724458/1‑69 (MQ=255) aCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg > 1:1273432/1‑69 (MQ=255) aCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg > 1:1417489/1‑69 (MQ=255) cAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGt > 1:3336316/1‑50 (MQ=255) cAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTcag > 1:3904249/1‑66 (MQ=255) cAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTc > 1:4035432/1‑66 (MQ=255) cAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg > 1:1907883/1‑68 (MQ=255) cAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg > 1:3690936/1‑68 (MQ=255) cAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg > 1:4716675/1‑68 (MQ=255) cAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg > 1:2544656/1‑68 (MQ=255) cAAAAACAAAGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg > 1:4307666/1‑68 (MQ=255) aaaaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa > 1:4189999/1‑69 (MQ=255) aaaaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa > 1:2582564/1‑69 (MQ=255) aaaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCa > 1:3467636/1‑60 (MQ=255) aaaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa > 1:3188188/1‑68 (MQ=255) aaaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa > 1:1947465/1‑68 (MQ=255) aaaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa > 1:2166856/1‑68 (MQ=255) aaaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa > 1:4838604/1‑68 (MQ=255) aaaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa > 1:3909642/1‑68 (MQ=255) aaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTg < 1:2529728/37‑1 (MQ=255) aaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTg < 1:3783730/37‑1 (MQ=255) aaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTg < 1:1701134/37‑1 (MQ=255) aaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa > 1:1081638/1‑67 (MQ=255) caacGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATc > 1:2834938/1‑70 (MQ=255) acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTAt > 1:3568936/1‑44 (MQ=16) acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTgcg > 1:3466443/1‑52 (MQ=19) acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc > 1:408721/1‑68 (MQ=255) acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc > 1:658818/1‑68 (MQ=255) acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc > 1:531556/1‑68 (MQ=255) acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc > 1:1061672/1‑68 (MQ=255) acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc > 1:4423473/1‑68 (MQ=255) acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc > 1:3615779/1‑68 (MQ=255) acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc > 1:1582473/1‑68 (MQ=255) acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc > 1:3124410/1‑68 (MQ=255) gAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAAt < 1:2536838/71‑1 (MQ=255) aGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCt < 1:4073208/71‑1 (MQ=255) ttCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGAtga < 1:4543288/56‑1 (MQ=255) ccTCGATCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGAtga < 1:1753935/54‑1 (MQ=255) cTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACgaga < 1:4069448/70‑1 (MQ=255) cTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACgaga < 1:2976790/70‑1 (MQ=255) cTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGaa < 1:736434/71‑1 (MQ=255) tCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACgaga < 1:3825626/69‑1 (MQ=255) tCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGaa < 1:2424165/70‑1 (MQ=255) cGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGaa < 1:3375686/69‑1 (MQ=255) tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa < 1:1879344/50‑1 (MQ=255) tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa < 1:1065409/50‑1 (MQ=255) tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa < 1:3965795/50‑1 (MQ=255) tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa < 1:709103/50‑1 (MQ=255) tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa < 1:337610/50‑1 (MQ=255) tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa < 1:1884815/50‑1 (MQ=255) tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAAtt < 1:2767203/64‑1 (MQ=255) tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAAt > 1:3043214/1‑71 (MQ=255) ttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa < 1:4195978/49‑1 (MQ=255) tACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGc > 1:2727891/1‑64 (MQ=255) tACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGc > 1:321991/1‑64 (MQ=255) tACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGc > 1:1378579/1‑64 (MQ=255) tACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGc > 1:4222585/1‑64 (MQ=255) tACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGc > 1:127299/1‑64 (MQ=255) tACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATca > 1:728910/1‑71 (MQ=255) tACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATca > 1:874782/1‑71 (MQ=255) aCATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa > 1:4687909/1‑38 (MQ=255) aCATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa > 1:2762159/1‑38 (MQ=255) aCATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa > 1:1406821/1‑38 (MQ=255) aCATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa > 1:4247604/1‑38 (MQ=255) cATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAACCTACGAGAAATTGc > 1:5107649/1‑62 (MQ=255) cATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAACCTACGAGAAATTGc > 1:3537176/1‑62 (MQ=255) | GAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCA > minE/1184944‑1185078 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |