| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,185,040 | T→G | 8.4% | I335M (ATT→ATG) ‡ | yeaG → | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,185,040 | 0 | T | G | 8.4% | 333.1 / 25.2 | 131 | I335M (ATT→ATG) ‡ | yeaG | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base T (50/70); new base G (9/2); total (59/72) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.26e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01 | |||||||||||
Only 100 of 141 total aligned reads displayed.
TAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTG > minE/1184974‑1185110 | taaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg < 1:4328090/71‑1 (MQ=255)taaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg < 1:2230309/71‑1 (MQ=255)taaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg < 1:4966717/71‑1 (MQ=255)taaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg < 1:1500764/71‑1 (MQ=255)taaCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg < 1:1347661/71‑1 (MQ=255) aCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg > 1:2724458/1‑69 (MQ=255) aCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg > 1:1417489/1‑69 (MQ=255) aCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg > 1:1273432/1‑69 (MQ=255) aCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg > 1:106081/1‑69 (MQ=255) aCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg > 1:308727/1‑69 (MQ=255) aCAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGACGTATTGCTTGCGCATTTCCg > 1:4213012/1‑69 (MQ=255) cAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTc > 1:4035432/1‑66 (MQ=255) cAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg > 1:1907883/1‑68 (MQ=255) cAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg > 1:2544656/1‑68 (MQ=255) cAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg > 1:3690936/1‑68 (MQ=255) cAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg > 1:4716675/1‑68 (MQ=255) cAAAAACAAAGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCg > 1:4307666/1‑68 (MQ=255) aaaaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa > 1:4189999/1‑69 (MQ=255) aaaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa > 1:3909642/1‑68 (MQ=255) aaaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa > 1:1947465/1‑68 (MQ=255) aaaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa > 1:2166856/1‑68 (MQ=255) aaaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa > 1:4838604/1‑68 (MQ=255) aaaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa > 1:3188188/1‑68 (MQ=255) aaaCAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa > 1:1081638/1‑67 (MQ=255) caacGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATc > 1:2834938/1‑70 (MQ=255) acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc > 1:408721/1‑68 (MQ=255) acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc > 1:3615779/1‑68 (MQ=255) acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc > 1:658818/1‑68 (MQ=255) acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc > 1:4423473/1‑68 (MQ=255) acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc > 1:1061672/1‑68 (MQ=255) acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc > 1:1582473/1‑68 (MQ=255) acGAGGCGTTCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGATGAAATc > 1:531556/1‑68 (MQ=255) aGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCt < 1:4073208/71‑1 (MQ=255) ttCCTCGACCGTATCTATATCGTGAAGGTGCCGTATTGCCTGCGCGTGTCCGAtga < 1:4543288/56‑1 (MQ=255) cTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACgaga < 1:2976790/70‑1 (MQ=255) cTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACgaga < 1:4069448/70‑1 (MQ=255) cTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGaa < 1:736434/71‑1 (MQ=255) tCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACgaga < 1:2323156/69‑1 (MQ=255) tCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACgaga < 1:2563254/69‑1 (MQ=255) tCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACgaga < 1:3825626/69‑1 (MQ=255) tCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGaa < 1:2424165/70‑1 (MQ=255) cGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGaa < 1:3375686/69‑1 (MQ=255) gtgtTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCtt < 1:1779497/71‑1 (MQ=255) gtgtTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCtt < 1:3686387/71‑1 (MQ=255) tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa < 1:1879344/50‑1 (MQ=255) tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa < 1:3965795/50‑1 (MQ=255) tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa < 1:4740196/50‑1 (MQ=255) tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa < 1:2447266/50‑1 (MQ=255) tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaaa < 1:1884815/50‑1 (MQ=255) tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAAtt < 1:2767203/64‑1 (MQ=255) tttACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAAt > 1:3043214/1‑71 (MQ=255) tACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGc > 1:1378579/1‑64 (MQ=255) tACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGc > 1:127299/1‑64 (MQ=255) tACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGc > 1:2727891/1‑64 (MQ=255) tACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGc > 1:321991/1‑64 (MQ=255) tACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGc > 1:4222585/1‑64 (MQ=255) tACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATca > 1:728910/1‑71 (MQ=255) tACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATca > 1:874782/1‑71 (MQ=255) aCATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa > 1:2762159/1‑38 (MQ=255) cATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAACCTACGAGAAATTGc > 1:4261839/1‑62 (MQ=255) gTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGGAATTGCTTAATCACAGTg < 1:2356672/70‑1 (MQ=255) gTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATca < 1:5196985/65‑1 (MQ=255) gTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATca < 1:2437766/65‑1 (MQ=255) gTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTg < 1:1840053/70‑1 (MQ=255) gTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTg < 1:3073442/70‑1 (MQ=255) gTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTg < 1:1781544/70‑1 (MQ=255) tGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGaa < 1:430561/71‑1 (MQ=255) tGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGaa < 1:2881223/71‑1 (MQ=255) aGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCtt < 1:1816405/56‑1 (MQ=255) aGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCtt < 1:5008081/56‑1 (MQ=255) aGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCtt < 1:174743/56‑1 (MQ=255) aGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCtt < 1:2472414/56‑1 (MQ=255) aGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTg < 1:1719619/71‑1 (MQ=255) aGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTg < 1:4285152/71‑1 (MQ=255) aGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTg < 1:5046679/71‑1 (MQ=255) aGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTg < 1:1040723/71‑1 (MQ=255) ggTGCCGTATTGCTTGCGCATTTCCGAAGAGATCa < 1:3574465/35‑1 (MQ=255) ggTGCCGTATTGCTTGCGCATTTCCGAAGAGATCa < 1:4930530/35‑1 (MQ=255) ggTGCCGTATTGCTTGCGCATTTCCGAAGAGATCa < 1:1076060/35‑1 (MQ=255) ggTGCCGTATTGCTTGCGCATTTCCGAAGAGATCa < 1:341536/35‑1 (MQ=255) ggTGCCGTATTGCTTGCGCATTTCCGAAGAGATCa < 1:2553684/35‑1 (MQ=255) ttgcttgcGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGccc > 1:2527608/1‑71 (MQ=255) ttgcttgcGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGccc > 1:1520917/1‑71 (MQ=255) gcttgcGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGaa > 1:2820106/1‑56 (MQ=255) gcttgcGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGaa > 1:625134/1‑56 (MQ=255) gcttgcGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGaa > 1:5121104/1‑56 (MQ=255) gcttgcGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCa > 1:4295035/1‑71 (MQ=255) ttgcGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTg < 1:5069451/52‑1 (MQ=255) tgcGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAAtt < 1:3288585/55‑1 (MQ=255) tgcGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGAGAAtt < 1:148272/55‑1 (MQ=255) gcATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATca < 1:850367/43‑1 (MQ=255) gcATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGaa < 1:4564777/50‑1 (MQ=255) tttCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCt < 1:3419402/71‑1 (MQ=255) tttCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCt < 1:4211307/71‑1 (MQ=255) tttCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCt < 1:2337622/71‑1 (MQ=255) ttCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTg > 1:3367641/1‑71 (MQ=255) ttCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTg > 1:2926631/1‑71 (MQ=255) ttCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTg > 1:1671366/1‑71 (MQ=255) ttCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTg > 1:1202735/1‑71 (MQ=255) ttCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTg > 1:1983160/1‑71 (MQ=255) | TAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCACAGTGAATTGACTCACGCCCCATGCGCCCCTG > minE/1184974‑1185110 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |