| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,191,074 | A→C | 9.6% | intergenic (+415/+485) | yeaJ → / ← rnd | predicted diguanylate cyclase/ribonuclease D |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,191,074 | 0 | A | C | 9.6% | 200.6 / 15.7 | 83 | intergenic (+415/+485) | yeaJ/rnd | predicted diguanylate cyclase/ribonuclease D |
| Reads supporting (aligned to +/- strand): ref base A (46/29); new base C (3/5); total (49/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTA > minE/1191009‑1191144 | aTGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAgcgc < 1:385864/71‑1 (MQ=255) tCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTag > 1:2439360/1‑71 (MQ=255) tCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTag > 1:3836259/1‑71 (MQ=255) aGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGt < 1:3838548/71‑1 (MQ=255) aGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGt > 1:3144890/1‑71 (MQ=255) aGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGt > 1:1128827/1‑71 (MQ=255) aGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGt > 1:2823985/1‑71 (MQ=255) aGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGt > 1:1188212/1‑71 (MQ=255) aGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGt > 1:2374440/1‑71 (MQ=255) tAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAgcg > 1:4502783/1‑57 (MQ=255) tAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAgcg > 1:4607299/1‑57 (MQ=255) aaGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTAc > 1:1468829/1‑71 (MQ=255) ggCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACaa > 1:2030400/1‑46 (MQ=255) ggCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTa < 1:1466175/52‑1 (MQ=255) ggCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTa < 1:3648065/52‑1 (MQ=255) ggCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTa < 1:1200429/52‑1 (MQ=255) ggCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTa < 1:4803076/52‑1 (MQ=255) ggCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTa < 1:2743847/52‑1 (MQ=255) ggCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTa < 1:390046/52‑1 (MQ=255) ggCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGt > 1:2649070/1‑67 (MQ=255) ggCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGa > 1:1627004/1‑69 (MQ=255) ggCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGa > 1:3282461/1‑69 (MQ=255) ggCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGa > 1:3774901/1‑69 (MQ=255) ggCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGa > 1:3705979/1‑69 (MQ=255) cTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGc < 1:3967781/71‑1 (MQ=255) cTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGc < 1:3952399/71‑1 (MQ=255) cTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGc < 1:1277437/71‑1 (MQ=255) cTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGc < 1:1429474/71‑1 (MQ=255) cTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGc < 1:3707596/71‑1 (MQ=255) cTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGc < 1:2613300/71‑1 (MQ=255) cTGCGGTAAGGCATCATCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGc < 1:2607085/71‑1 (MQ=255) gTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAg < 1:1159833/65‑1 (MQ=255) aaGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACaa > 1:2766268/1‑37 (MQ=255) aaGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACaa > 1:3016226/1‑37 (MQ=255) aaGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACaa > 1:4752435/1‑37 (MQ=255) aaGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACaa > 1:4000622/1‑37 (MQ=255) gCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCt < 1:2908969/71‑1 (MQ=255) tCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTc > 1:1259917/1‑65 (MQ=255) tCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTc > 1:2206013/1‑65 (MQ=255) tCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTc > 1:1266074/1‑65 (MQ=255) tCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTc > 1:3186529/1‑65 (MQ=255) tCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTc > 1:1051114/1‑65 (MQ=255) ttCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTag > 1:5132314/1‑36 (MQ=255) ttCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCg > 1:981970/1‑70 (MQ=255) ttCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCg > 1:3257066/1‑70 (MQ=255) ttCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCg > 1:1102630/1‑70 (MQ=255) ttCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCg > 1:2718790/1‑70 (MQ=255) ttCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCg > 1:1458252/1‑70 (MQ=255) ttCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCg > 1:1254692/1‑70 (MQ=255) ttCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCg > 1:2336976/1‑70 (MQ=255) ttCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCg > 1:2415069/1‑70 (MQ=255) ttCCGG‑AATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGa > 1:4001034/1‑71 (MQ=255) ccGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGa > 1:4171470/1‑50 (MQ=255) ccGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGa > 1:2861723/1‑70 (MQ=255) ccGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGa > 1:268247/1‑70 (MQ=255) ccGGCAATGTCTGCGCTTTTTCCACAAGCGCTagc > 1:4031149/1‑35 (MQ=255) ccGGCAATGTCTGCGCTTTTTCCACAAGCGCTagc > 1:1473988/1‑35 (MQ=255) ccGGCAATGTCTGCGCTTTTTCCACAAGCGCTagc > 1:3364821/1‑35 (MQ=255) ccGGCAATGTCTGCGCTTTTTCCACAAGCGCTagc > 1:3189356/1‑35 (MQ=255) cGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATa < 1:589503/71‑1 (MQ=255) cGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATa < 1:1582787/71‑1 (MQ=255) ggCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATa > 1:3361730/1‑70 (MQ=255) cAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAAc > 1:1946850/1‑71 (MQ=255) cAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAAc > 1:88019/1‑71 (MQ=255) aaTGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTg < 1:3835397/44‑1 (MQ=255) aaTGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTg < 1:4981982/44‑1 (MQ=255) aaTGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTg < 1:3110853/44‑1 (MQ=255) aaTGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATaaa > 1:1187337/1‑69 (MQ=255) aaTGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATaaa > 1:3333972/1‑69 (MQ=255) aaTGTCTGCGCTGTTTCCACAAGCGCTAGCAGCGTTTTACCGTg < 1:3407316/44‑1 (MQ=255) gTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAg > 1:3171586/1‑71 (MQ=255) gTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAg > 1:2164819/1‑71 (MQ=255) tCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGc > 1:4199398/1‑54 (MQ=255) tCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGc > 1:1611879/1‑54 (MQ=255) tCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGc > 1:2532299/1‑54 (MQ=255) cgcTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCt < 1:4176423/69‑1 (MQ=255) cgcTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCt < 1:1849054/69‑1 (MQ=255) cgcTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTg < 1:31917/70‑1 (MQ=255) cgcTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTg < 1:542936/70‑1 (MQ=255) cgcTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTCAAAGCGGATTTCGCTACCGGATAAACCCAGGCTg < 1:4693089/70‑1 (MQ=255) cTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCg < 1:2983510/40‑1 (MQ=255) cTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCg < 1:774652/40‑1 (MQ=255) cTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCg < 1:739165/40‑1 (MQ=255) cTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCg < 1:3924470/40‑1 (MQ=255) cTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCg < 1:1825618/40‑1 (MQ=255) cTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCg < 1:4205098/40‑1 (MQ=255) tttCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGt > 1:5066483/1‑71 (MQ=255) tttCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGt > 1:2343796/1‑71 (MQ=255) cacaAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCg > 1:4803888/1‑70 (MQ=255) cacaAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGc > 1:4401485/1‑71 (MQ=255) aaGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTa < 1:2212229/71‑1 (MQ=255) | ATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTA > minE/1191009‑1191144 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |