| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,305,362 | T→C | 15.1% | T233A (ACG→GCG) | yeeE ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,305,362 | 0 | T | C | 15.1% | 125.6 / 13.7 | 53 | T233A (ACG→GCG) | yeeE | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (20/25); new base C (3/5); total (23/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGGAAAACGCCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCG > minE/1305297‑1305427 | cAGGAAAACGCCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTgggg < 1:1873821/71‑1 (MQ=255)cAGGAAAACGCCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTgggg < 1:3487806/71‑1 (MQ=255)cAGGAAAACGCCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTgggg < 1:2178872/71‑1 (MQ=255)cAGGAAAACGCCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTgggg < 1:2828549/71‑1 (MQ=255)cAGGAAAACGCCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTggg > 1:2078642/1‑70 (MQ=255) aaaaCGCCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGt < 1:503956/71‑1 (MQ=255) aaaaCGCCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGt < 1:3355133/71‑1 (MQ=255) gCCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTc < 1:4767852/71‑1 (MQ=255) gCCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTc < 1:2862365/71‑1 (MQ=255) ccccAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGAt < 1:1238515/68‑1 (MQ=255) ccAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTa < 1:186714/71‑1 (MQ=255) gTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTgggg < 1:1481100/56‑1 (MQ=255) aTGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCaaa < 1:4427513/71‑1 (MQ=255) tGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCaaa < 1:3487624/70‑1 (MQ=255) tATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCTAACAt > 1:1193333/1‑71 (MQ=255) tATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACAt > 1:1975007/1‑71 (MQ=255) tATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACAt > 1:581691/1‑71 (MQ=255) tATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACAt > 1:2915586/1‑71 (MQ=255) tttCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGc > 1:781541/1‑71 (MQ=255) gcgACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCaaa > 1:4992606/1‑53 (MQ=255) gcgACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCtt > 1:4326255/1‑70 (MQ=255) aCCAGAAATTGCAGAATATTGGCCGCTGGTGAAGTGATTCCTAACCCaaa < 1:4908383/50‑1 (MQ=38) aCCAGAAATTGCAGAATATTGGCCGCTGGGGAAGTGATTCCTAACCCaaa < 1:2888344/50‑1 (MQ=255) aCCAGAAATTGCAGAATATTGGCCGCTGGGGAAGTGATTCCTAACCCaaa < 1:4648367/50‑1 (MQ=255) aCCAGAAATTGCAGAATATTGGCCGCTGGGGAAGTGATTCCTAACCCaaa < 1:4564930/50‑1 (MQ=255) aCCAGAAATTGCAGAATATTGGCCGCTGGGGAAGTGATTCCTAACCCaaa < 1:453073/50‑1 (MQ=255) cAGAAATTGCAGAATATTGGCCTCTGGGGAAGTGATTCCTAACCCAAACa > 1:434920/1‑50 (MQ=255) cAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCaaa < 1:2981440/48‑1 (MQ=255) cAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCaaa < 1:25822/48‑1 (MQ=255) cAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCaaa < 1:3542989/48‑1 (MQ=255) cAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCaaa < 1:2593280/48‑1 (MQ=255) cAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCa < 1:1100004/71‑1 (MQ=255) cAGAAATTGCAGAATATTGGCCGCTGGGGAAGTGATTCCTAACCCAAACa > 1:4360821/1‑50 (MQ=255) cAGAAATTGCAGAATATTGGCCGCTGGGGAAGTGATTCCTAACCCAAACa > 1:398463/1‑50 (MQ=255) gAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAgg < 1:3970176/71‑1 (MQ=255) gAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAgg < 1:4509993/71‑1 (MQ=255) aaaTTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAggg < 1:447658/71‑1 (MQ=255) aTTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGc < 1:3407456/71‑1 (MQ=255) gCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCaa > 1:4141191/1‑39 (MQ=255) gCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAg > 1:2686056/1‑71 (MQ=255) gCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAg > 1:558951/1‑71 (MQ=255) gCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAg > 1:1543503/1‑71 (MQ=255) gAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGc > 1:3862897/1‑70 (MQ=255) gAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGc > 1:1443054/1‑70 (MQ=255) aaTATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCt > 1:3310053/1‑70 (MQ=255) aaTATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCt > 1:4073393/1‑70 (MQ=255) ttGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGC‑TCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAg < 1:3108894/70‑1 (MQ=255) tGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGc > 1:2913286/1‑36 (MQ=255) tGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGtt > 1:2607566/1‑44 (MQ=255) tGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTaaaa > 1:4636626/1‑69 (MQ=255) ggCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAggg < 1:3848132/56‑1 (MQ=255) ggCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAgcg > 1:2391326/1‑71 (MQ=255) ccGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCa < 1:3704180/51‑1 (MQ=255) ttGGGGAAGTGATTCCTAACCCAAACATGCGTCCgg < 1:215481/36‑1 (MQ=255) ttGGGGAAGTGATTCCTAACCCAAACATGCGTCCgg < 1:3378493/36‑1 (MQ=255) ttGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTaa < 1:3568354/61‑1 (MQ=255) | CAGGAAAACGCCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCG > minE/1305297‑1305427 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |