Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 1,305,959 | A→G | 6.7% | Y34H (TAT→CAT) | yeeE ← | predicted inner membrane protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 1,305,959 | 0 | A | G | 6.7% | 222.1 / 5.3 | 74 | Y34H (TAT→CAT) | yeeE | predicted inner membrane protein |
Reads supporting (aligned to +/- strand): ref base A (26/43); new base G (2/3); total (28/46) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01 |
AAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGCACCACA > minE/1305889‑1306028 | aaaaCCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGAtata < 1:3291725/71‑1 (MQ=255) aaaCCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATAc < 1:2086725/71‑1 (MQ=255) aCCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACat < 1:4810692/71‑1 (MQ=255) cccccACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACata < 1:4326728/71‑1 (MQ=255) cccccACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACata < 1:4951621/71‑1 (MQ=255) cccccACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACata < 1:3931386/71‑1 (MQ=255) ccccACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACata < 1:1246210/70‑1 (MQ=255) gCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGaa < 1:3746792/71‑1 (MQ=255) gCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGaa < 1:3586935/71‑1 (MQ=255) gCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGaa < 1:2814528/71‑1 (MQ=255) gCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGaa < 1:2760997/71‑1 (MQ=255) gCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGaa < 1:5160418/71‑1 (MQ=255) cTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGaa > 1:439366/1‑70 (MQ=255) cTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGaa > 1:4457464/1‑70 (MQ=255) cTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGaa > 1:4604796/1‑70 (MQ=255) cTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGaa > 1:3187694/1‑70 (MQ=255) cTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGaa > 1:3186079/1‑70 (MQ=255) tGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAac > 1:572557/1‑70 (MQ=255) gTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAacca > 1:2479079/1‑71 (MQ=255) gCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGc < 1:902378/70‑1 (MQ=255) gCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGc < 1:4621231/70‑1 (MQ=255) tCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCaaaaa > 1:3420142/1‑71 (MQ=255) tCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCaaaaa > 1:2040608/1‑71 (MQ=255) aGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCa > 1:1101156/1‑61 (MQ=255) aGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCa > 1:1382139/1‑61 (MQ=255) aGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCa > 1:5169781/1‑61 (MQ=255) aGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCa > 1:2644936/1‑61 (MQ=255) aGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAAc < 1:838385/70‑1 (MQ=255) aGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAAc < 1:2771751/70‑1 (MQ=255) aGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACg > 1:80739/1‑71 (MQ=255) aGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACg > 1:1588494/1‑71 (MQ=255) aGCAAGGCGTAAAACATGAGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCa > 1:1265188/1‑61 (MQ=255) gCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACg > 1:1353956/1‑71 (MQ=255) tAAAACATGCGATTATTTTTCACGATATACATATCGCGaa < 1:3347921/40‑1 (MQ=255) tAAAACATGCGATTATTTTTCACGATATACATATCGCGaa < 1:4246192/40‑1 (MQ=255) tAAAACATGCGATTATTTTTCACGATATACATATCGCGaa < 1:692412/40‑1 (MQ=255) tAAAACATGCGATTATTTTTCACGATATACATATCGCGaa < 1:177111/40‑1 (MQ=255) tAAAACATGCGATTATTTTTCACGATATACATATCGCGaa < 1:1390294/40‑1 (MQ=255) tAAAACATGCGATTATTTTTCACGATATACATATCGCGaa < 1:860585/40‑1 (MQ=255) tAAAACATGCGATTATTTTTCACGATATACATATCGCGaa < 1:2393029/40‑1 (MQ=255) tAAAACATGCGATTATTTTTCACGATATACATATCGCGaa < 1:4872831/40‑1 (MQ=255) tAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTc < 1:3235217/51‑1 (MQ=255) tAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTc < 1:4630912/51‑1 (MQ=255) tAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTc < 1:1009198/51‑1 (MQ=255) tAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTc < 1:2061096/51‑1 (MQ=255) tAAAACATGCGATTATTTTTCACGATATAAATATCGCGaa < 1:2262307/40‑1 (MQ=255) aaaaCATGCGATTATTTTTCACGATATACATATCGCGaa < 1:827242/39‑1 (MQ=255) aaaaCATGCGATTATTTTTCACGATATACATATCGCGaa < 1:4799582/39‑1 (MQ=255) aaaaCATGCGATTATTTTTCACGATATACATATCGCGaa < 1:3041435/39‑1 (MQ=255) aaaCATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTc > 1:3214995/1‑49 (MQ=255) aaaCATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTc > 1:1871662/1‑49 (MQ=255) aaaCATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTc > 1:1232850/1‑49 (MQ=255) aaaCATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTc > 1:618475/1‑49 (MQ=255) ttattTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCa < 1:918220/44‑1 (MQ=255) ttattTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCa < 1:3919602/44‑1 (MQ=255) ttattTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCa < 1:1823206/44‑1 (MQ=255) ttattTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATcc < 1:3587608/71‑1 (MQ=255) tattTTTCACGATATACATATCGCGAAAACCACCTGTCAg > 1:3889553/1‑40 (MQ=255) tattTTTCACGATATACATATCGCGAAAACCACCTGTCAg > 1:1527246/1‑40 (MQ=255) tattTTTCACGATATACATATCGCGAAAACCACCTGTCAg > 1:2346320/1‑40 (MQ=255) tattTTTCACGATATACATATCGCGAAAACCACCTGTCAg > 1:404673/1‑40 (MQ=255) tattTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATcc > 1:1476000/1‑70 (MQ=255) tttttCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCcag < 1:2490811/71‑1 (MQ=255) tttttCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCcag < 1:1906756/71‑1 (MQ=255) tttttCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCcag < 1:2357071/71‑1 (MQ=255) tttttCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCcag < 1:2380298/71‑1 (MQ=255) tttttCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCcag < 1:4823649/71‑1 (MQ=255) tttttCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCcag < 1:3518613/71‑1 (MQ=255) ttttCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCcag < 1:1140211/70‑1 (MQ=255) tttCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCcag < 1:3785861/69‑1 (MQ=255) cgcGATATGTATATCGTGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCagag > 1:4712559/3‑71 (MQ=38) cgcGATATGTATATCGTGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCagag > 1:1532021/3‑71 (MQ=38) gcGATATGTATATCGTGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCagag < 1:2801422/69‑1 (MQ=38) gcGATATGTATATCGTGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCagag < 1:3318746/69‑1 (MQ=38) gcGATATGTATATCGTGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCagag < 1:1183334/69‑1 (MQ=38) taCATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGcaccac > 1:3588689/1‑70 (MQ=255) taCATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGcaccac > 1:1704623/1‑70 (MQ=255) taCATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGcaccac > 1:3839646/1‑70 (MQ=255) taCATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGCACcaca < 1:859913/71‑1 (MQ=255) taCATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGCACcaca < 1:3850982/71‑1 (MQ=255) | AAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGCACCACA > minE/1305889‑1306028 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |