| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,319,262 | G→A | 10.1% | Y210Y (TAC→TAT) ‡ | ugd ← | UDP‑glucose 6‑dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,319,262 | 0 | G | A | 10.1% | 342.1 / 22.3 | 119 | Y210Y (TAC→TAT) ‡ | ugd | UDP‑glucose 6‑dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (41/66); new base A (5/7); total (46/73) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
Only 100 of 125 total aligned reads displayed.
GACAAACGCCTTCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAA > minE/1319192‑1319332 | gaCAAACGCCTTCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAg > 1:527932/1‑71 (MQ=255)gaCAAACGCCTTCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAg < 1:643484/71‑1 (MQ=255) aaaCGCCTTCGTTTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTAc < 1:368695/71‑1 (MQ=255) aaaCGCCTTCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTAc < 1:848508/71‑1 (MQ=255) aaCGCCTTCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTAc < 1:1222991/70‑1 (MQ=255) aaCGCCTTCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACg > 1:3739270/1‑71 (MQ=255) tCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCAcgcg > 1:1831661/1‑71 (MQ=255) cGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCAcg < 1:4319808/68‑1 (MQ=255) tttGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGcc < 1:573175/71‑1 (MQ=255) tttGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGcc < 1:3166188/71‑1 (MQ=255) tttGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGcc < 1:3682266/71‑1 (MQ=255) gAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggtaggt > 1:4439288/1‑71 (MQ=255) gAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggtaggt > 1:2953928/1‑71 (MQ=255) ttCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGtt < 1:3818423/71‑1 (MQ=255) tCAGACCAAAACTTTCTGCGTAACTATCCAATTCATTAAAAAATGCAACTCGCATAGCCaa > 1:4607805/1‑60 (MQ=255) tCAGACCAAAACTTTCTGAGTAACTATCCAATTCATTAAAAAATGCAACTCGCATAGCCaa > 1:287557/1‑60 (MQ=255) tCAGACCAAAACTTTCTGAGTAACTATCCAATTCATTAAAAAATGCAACTCGCATAGCCaa > 1:4681044/1‑60 (MQ=255) tCAGACCAAAACTTTCTGAGTAACTATCCAATTCATTAAAAAATGCAACTCGCATAGCCaa > 1:2217858/1‑60 (MQ=255) ccTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCaaa < 1:5170976/71‑1 (MQ=255) ccTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCaaa < 1:690645/71‑1 (MQ=255) aaaCTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCAc > 1:443891/1‑41 (MQ=255) aaaCTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCAt > 1:2010709/1‑47 (MQ=255) aaaCTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCAt > 1:3581539/1‑47 (MQ=255) aaaCTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCAt > 1:1373500/1‑47 (MQ=255) aaaCTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGttt > 1:3566403/1‑63 (MQ=255) aaaCTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGttt > 1:2684495/1‑63 (MQ=255) aaaCTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGttt > 1:1921521/1‑63 (MQ=255) aaaCTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGttt > 1:3589920/1‑63 (MQ=255) aaaCTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGACAGGTAGGTGTTTGCaa > 1:2159600/1‑67 (MQ=255) aCTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGtt > 1:2494079/1‑71 (MQ=255) aCTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGtt > 1:4884383/1‑71 (MQ=255) cTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGtt > 1:2909430/1‑70 (MQ=255) tttCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggtaggt < 1:1840174/55‑1 (MQ=255) tttCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggtaggt < 1:3768706/55‑1 (MQ=255) tttCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggtaggt < 1:1103894/55‑1 (MQ=255) tttCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggtaggt < 1:4767132/55‑1 (MQ=255) tttCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggtaggt < 1:1234297/55‑1 (MQ=255) tttCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggtaggt < 1:4812659/55‑1 (MQ=255) tttCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggtaggt < 1:582410/55‑1 (MQ=255) tttCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggtaggt < 1:1932202/55‑1 (MQ=255) tttCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggtaggt < 1:1501587/55‑1 (MQ=255) tttCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggtaggt < 1:3383016/55‑1 (MQ=255) tCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTa < 1:1005338/69‑1 (MQ=255) tCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTa < 1:3315514/69‑1 (MQ=255) tCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTa < 1:3252656/69‑1 (MQ=255) tCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTa < 1:3203773/69‑1 (MQ=255) tCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTa < 1:649933/69‑1 (MQ=255) tCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTa < 1:2043141/69‑1 (MQ=255) cTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTa < 1:5011502/68‑1 (MQ=255) cATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGc < 1:4969544/70‑1 (MQ=255) gCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTg < 1:1661509/71‑1 (MQ=255) acTATCCAATTCATTAAAAAATGCAACTCGCATAGCCAAATAAGTATTTGAAAATAACTTAATCGCTTccg < 1:615495/70‑3 (MQ=255) acTATCCAATTCATTAAAAAATGCAACTCGCATAGCCAAATAAGTATTTGAAAATAACTTAATCGCTTccg < 1:4724149/70‑3 (MQ=255) acTATCCAATTCATTAAAAAATGCAACTCGCATAGCCAAATAAGTATTTGAAAATAACTTAATCGCTTccg < 1:2299047/70‑3 (MQ=255) acTATCCAATTCATTAAAAAATGCAACTCGCATAGCCAAATAAGTATTTGAAAATAACTTAATCGCTTccg < 1:755333/70‑3 (MQ=255) tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggta < 1:4947300/38‑1 (MQ=255) tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggta < 1:399514/38‑1 (MQ=255) tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggta < 1:4966096/38‑1 (MQ=255) tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggta < 1:4846742/38‑1 (MQ=255) tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggta < 1:146121/38‑1 (MQ=255) tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggta < 1:899588/38‑1 (MQ=255) tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggta < 1:4576582/38‑1 (MQ=255) tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggta < 1:4524427/38‑1 (MQ=255) tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggta < 1:3055388/38‑1 (MQ=255) tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggta < 1:4213133/38‑1 (MQ=255) tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggta < 1:3452786/38‑1 (MQ=255) tCGTTAGAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTg > 1:3431117/1‑69 (MQ=255) tCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTg > 1:62049/1‑40 (MQ=255) tCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAAt > 1:4666664/1‑53 (MQ=255) tCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAAt > 1:4340832/1‑53 (MQ=255) tCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAAt > 1:1623029/1‑53 (MQ=255) tCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAAt > 1:902440/1‑53 (MQ=255) tCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAAt > 1:370886/1‑53 (MQ=255) tCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTg > 1:4897004/1‑61 (MQ=255) tCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTg > 1:1084515/1‑69 (MQ=255) ttAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCaaa < 1:1012509/41‑1 (MQ=255) ttAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCaaa < 1:1573411/41‑1 (MQ=255) ttAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCaaa < 1:2759465/41‑1 (MQ=255) ttAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCaaa < 1:306440/41‑1 (MQ=255) ttAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCaaa < 1:4056243/41‑1 (MQ=255) ttAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCaaa < 1:3347611/41‑1 (MQ=255) ttAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCaaa < 1:4009603/41‑1 (MQ=255) ttAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCaaa < 1:379266/41‑1 (MQ=255) tAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCg > 1:5109549/1‑71 (MQ=255) tAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCg > 1:5127016/1‑71 (MQ=255) aaaGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCa > 1:4780284/1‑61 (MQ=255) aaaGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCa > 1:921745/1‑61 (MQ=255) aaaGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCa > 1:830104/1‑61 (MQ=255) aaaGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCa > 1:402859/1‑61 (MQ=255) aaaGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCa > 1:4990790/1‑61 (MQ=255) aGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTa < 1:3118105/71‑1 (MQ=255) aGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTa < 1:5036445/71‑1 (MQ=255) aGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTa < 1:752009/71‑1 (MQ=255) aGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTa < 1:2839251/71‑1 (MQ=255) aGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTa < 1:425485/71‑1 (MQ=255) aGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTa < 1:5001770/71‑1 (MQ=255) aGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTa < 1:2149883/71‑1 (MQ=255) aGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTa < 1:4906168/71‑1 (MQ=255) gTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTaa < 1:3381302/71‑1 (MQ=255) gTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTaa < 1:2956482/71‑1 (MQ=255) | GACAAACGCCTTCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAA > minE/1319192‑1319332 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |