| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,336,113 | G→A | 10.4% | L270L (CTG→TTG) | gmd ← | GDP‑D‑mannose dehydratase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,336,113 | 0 | G | A | 10.4% | 436.8 / 30.7 | 153 | L270L (CTG→TTG) | gmd | GDP‑D‑mannose dehydratase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base G (64/73); new base A (5/11); total (69/84) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01 | |||||||||||
Only 100 of 162 total aligned reads displayed.
CCGGTGACGGAAACCACAATGCCCTTCTCTTCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGTACTGAACGCCGGTCGCGATAACGAAAT > minE/1336046‑1336178 | ggAAACCACAATGCCCTTCTCTTCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTgccg > 1:80973/1‑71 (MQ=255) ggAAACCACAATGCCCTTCTCTTCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTgccg > 1:713474/1‑71 (MQ=255) ggAAACCACAATGCCCTTCTCTTCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTgccg > 1:455045/1‑71 (MQ=255) aaaCCACAATGCCCTTCTCTTCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTgccgcc < 1:2730720/71‑1 (MQ=255) aCCACAATGCCCTTCTCTTCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCAt > 1:2770888/1‑71 (MQ=255) aCCACAATGCCCTTCTCTTCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCAt > 1:4793468/1‑71 (MQ=255) aCCACAATGCCCTTCTCTTCAACGCCCGTGCCTTAAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCAt > 1:3648445/1‑71 (MQ=255) cacaATGCCCTTCTCTTCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCAttt > 1:1060724/1‑71 (MQ=255) cacaATGCCCTTCTCTTCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCAttt > 1:307863/1‑71 (MQ=255) cacaATGCCCTTCTCTTCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCAttt > 1:1549025/1‑71 (MQ=255) acaATGCCCTTCTCTTCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTc > 1:1508556/1‑71 (MQ=255) acaATGCCCTTCTCTTCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTc > 1:3606133/1‑71 (MQ=255) acaATGCCCTTCTCTTCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTc > 1:3496075/1‑71 (MQ=255) tGCCCTTCTCTTCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACg < 1:4645395/71‑1 (MQ=255) tGCCCTTCTCTTCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACg < 1:1257997/71‑1 (MQ=255) tGCCCTTCTCTTCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACg < 1:2368511/71‑1 (MQ=255) tctcTTCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCa < 1:3004111/57‑1 (MQ=255) tctcTTCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGa > 1:1607677/1‑71 (MQ=255) ctctTCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCtgtg > 1:2478388/1‑47 (MQ=255) ctctTCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCtgtg > 1:2470407/1‑47 (MQ=255) ctctTCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCtgtg > 1:2144714/1‑47 (MQ=255) ctctTCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGa > 1:3108874/1‑70 (MQ=255) ctctTCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGAc > 1:3780927/1‑71 (MQ=255) ctctTCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGAc > 1:5116357/1‑71 (MQ=255) ctTCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGtgctg < 1:3356885/48‑1 (MQ=255) ctTCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGtgctg < 1:3438105/48‑1 (MQ=255) ctTCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGtgctg < 1:140361/48‑1 (MQ=255) ttCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCtg < 1:4125037/42‑1 (MQ=255) ttCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCtg < 1:4160288/42‑1 (MQ=255) ttCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCtg < 1:3732724/42‑1 (MQ=255) ttCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCtg < 1:1436432/42‑1 (MQ=255) ttCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCtg < 1:770827/42‑1 (MQ=255) ttCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCtg < 1:15948/42‑1 (MQ=255) ttCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCtg < 1:3294212/42‑1 (MQ=255) tCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTgcc > 1:1312700/1‑48 (MQ=255) tCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGACGCAc > 1:2052238/1‑71 (MQ=255) tCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGACGCAc > 1:794486/1‑71 (MQ=255) tCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGACGCAc > 1:288705/1‑71 (MQ=255) cAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCGGc > 1:2244365/1‑38 (MQ=255) cAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGc > 1:3135556/1‑38 (MQ=255) cAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGc > 1:4462611/1‑38 (MQ=255) cAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGc > 1:4960995/1‑38 (MQ=255) cAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGc > 1:2940289/1‑38 (MQ=255) cAACACCCGTGCCTTCAAAGCGCAGTTTGATGCCCAACTGTGCTGCCGCCATTTCCACGAACTGACgt < 1:165343/68‑2 (MQ=255) cAACACCCGTGCCTTCAAAGCGCAGTTTGATGCCCAACTGTGCTGCCGCCATTTCCACGAACTGACgt < 1:1193382/68‑2 (MQ=255) cAACACCCGTGCCTTCAAAGCGCAGTTTGATGCCCAACTGTGCTGCCGCCATTTCCACGAACTGACgt < 1:5173316/68‑2 (MQ=255) cAACACCCGTGCCTTCAAAGCGCAGTTTGATGCCCAACTGTGCTGCCGCCATTTCCACGAACTGACgt < 1:173078/68‑2 (MQ=255) cAACACCCGTGCCTTCAAAGCGCAGTTTGATGCCCAACTGTGCTGCCGCCATTTCCACGAACTGACgt < 1:1995737/68‑2 (MQ=255) cAACACCCGTGCCTTCAAAGCGCAGTTTGATGCCCAACTGTGCTGCCGCCATTTCCACGAACTGACgt < 1:538020/68‑2 (MQ=255) cAACACCCGTGCCTTCAAAGCGCAGTTTGATGCCCAACTGTGCTGCCGCCATTTCCACGAACTGACgt < 1:4267507/68‑2 (MQ=255) cAACACCCGTGCCTTCAAAGCGCAGTTTGATGCCCAACTGTGCTGCCGCCATTTCCACGAACTGACgt < 1:4321552/68‑2 (MQ=255) cAACACCCGTGCCTTCAAAGCGCAGTTTGATGCCCAACTGTGCTGCCGCCATTTCCACGAACTGACgt < 1:4580716/68‑2 (MQ=255) aaCGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCtgtg > 1:759739/1‑41 (MQ=255) aaCGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCtgtg > 1:1956075/1‑41 (MQ=255) aaCGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACg < 1:2247107/70‑1 (MQ=255) aaCGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACg < 1:3452984/70‑1 (MQ=255) cGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAg < 1:2612995/71‑1 (MQ=255) cccGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACg < 1:2501729/66‑1 (MQ=255) cttGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACg < 1:171288/50‑1 (MQ=255) cGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTcct < 1:1527500/50‑2 (MQ=255) cGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACg < 1:1466755/52‑1 (MQ=255) cGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACg < 1:3549130/52‑1 (MQ=255) cGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACg < 1:4210895/52‑1 (MQ=255) gTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGtgctgc < 1:5111373/38‑1 (MQ=255) gTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGTACTg < 1:991716/71‑1 (MQ=255) ccTTCAAAGCGCAGTTTGATGCCCAGGTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGTACTGAAc > 1:4827957/1‑71 (MQ=255) ccTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGTACTGAAc > 1:149908/1‑71 (MQ=255) ccTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGTACTGAAc > 1:295615/1‑71 (MQ=255) cTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGTACTGAACg < 1:844921/71‑1 (MQ=255) cTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGTACTGAACg < 1:5013153/71‑1 (MQ=255) cAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGTACTGAACGc < 1:1471568/69‑1 (MQ=255) cAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGTACTGAACGc < 1:4550885/69‑1 (MQ=255) aaaGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACGaa > 1:4249632/1‑45 (MQ=255) aGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGTACTGAACGCCGGTc < 1:3606415/71‑1 (MQ=255) gcgcAGTTTGATGCCCAGCTGTGCTGCCGCCATTTc < 1:2181520/36‑1 (MQ=255) aGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGTACTg < 1:5170922/56‑1 (MQ=255) aGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGTACTg < 1:5085565/56‑1 (MQ=255) tttGATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGTACTGaa > 1:3293264/1‑56 (MQ=255) tttGATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGTACTGaa > 1:3709925/1‑56 (MQ=255) tttGATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGTACTGaa > 1:1559917/1‑56 (MQ=255) tttGATGCCCAGCTGTGCTGCCGCCATTTCAACGAACTGACGCACGGAGTACTGaa > 1:3635105/1‑56 (MQ=255) tGATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGTACTGAACGCCGGTCGCGATAAc < 1:3967896/70‑1 (MQ=255) gATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGTACTGAACGCCGGTCGCGATAACg < 1:3680593/70‑1 (MQ=255) gATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGTACTGAACGCCGGTCGCGATAACg < 1:673101/70‑1 (MQ=255) aTGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGTGCTGAACGCCGGTCGCGATAACGaa < 1:3515424/71‑1 (MQ=255) cccAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGt < 1:2318228/43‑1 (MQ=255) cccAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGt < 1:4651744/43‑1 (MQ=255) cccAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGt < 1:909671/43‑1 (MQ=255) cccAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGt < 1:5195806/43‑1 (MQ=255) cccAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGt < 1:2549687/43‑1 (MQ=255) cccAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGTACTGaa > 1:4778016/1‑49 (MQ=255) cccAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGTACTGaa > 1:2754845/1‑49 (MQ=255) cccAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGTACTGaa > 1:3182794/1‑49 (MQ=255) cAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGTACTGAACGCCGGTCGCGATAACGAAAt > 1:3055769/1‑68 (MQ=255) gCTGTGCTGCCGCCATTTCCACGAACTGACGCACg < 1:1821600/35‑1 (MQ=255) gCTGTGCTGCCGCCATTTCCACGAACTGACGCACg < 1:768477/35‑1 (MQ=255) gCTGTGCTGCCGCCATTTCCACGAACTGACGCACg < 1:4446835/35‑1 (MQ=255) gCTGTGCTGCCGCCATTTCCACGAACTGACGCACg < 1:3991428/35‑1 (MQ=255) gCTGTGCTGCCGCCATTTCCACGAACTGACGCACg < 1:1507206/35‑1 (MQ=255) gCTGTGCTGCCGCCATTTCCACGAACTGACGCACg < 1:1041253/35‑1 (MQ=255) | CCGGTGACGGAAACCACAATGCCCTTCTCTTCAACGCCCGTGCCTTCAAAGCGCAGTTTGATGCCCAGCTGTGCTGCCGCCATTTCCACGAACTGACGCACGGAGTACTGAACGCCGGTCGCGATAACGAAAT > minE/1336046‑1336178 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |