| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,488,647 | A→C | 9.5% | V127V (GTT→GTG) | nuoM ← | NADH:ubiquinone oxidoreductase, membrane subunit M |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,488,647 | 0 | A | C | 9.5% | 232.8 / 20.4 | 95 | V127V (GTT→GTG) | nuoM | NADH:ubiquinone oxidoreductase, membrane subunit M |
| Reads supporting (aligned to +/- strand): ref base A (55/31); new base C (4/5); total (59/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
Only 100 of 104 total aligned reads displayed.
ATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAA > minE/1488579‑1488716 | aTCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGATGGCAAGGAACACGCCGATaa > 1:915908/1‑70 (MQ=255)aTCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGATGGCAAGGAACACGCCGATAAc > 1:4331577/1‑71 (MQ=255) aGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGATGGCAAGGAACACGCCGATaa > 1:3902023/1‑61 (MQ=255) aGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGATGGCAAGGAACACGCCGATaa > 1:2596952/1‑61 (MQ=255) aGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGATGGCAAGGAACACGCCGATaa > 1:2610378/1‑61 (MQ=255) aGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGATGGCAAGGAACACGCCGATaa > 1:1655390/1‑61 (MQ=255) aGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAg > 1:4533502/1‑71 (MQ=255) tcatTTCCCAGAAGAAGAAGAACAGGAACATGTCGATGGCAAGGAACACGCCCATAACGCCGCCCAGGATc < 1:1945249/71‑1 (MQ=255) atTTCCCAGAAGAAGAAGAACAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATcc < 1:1659856/70‑1 (MQ=255) tttCCCAGAAGAAGAAGAACAGGAACAGGTCGATGGCGAGGAACACGCCGATCACACCGCCCAGGATCcac < 1:3030360/71‑1 (MQ=255) agaagaagaagaACAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAgg > 1:3551469/1‑71 (MQ=255) aagaagaagaaCAGGAACAGGTCGATGGCGAGGAACACGCCGATCACACCGCCCAGGATCca < 1:2768686/62‑1 (MQ=255) aagaagaagaaCAGGAACAGGTCGATGGCGAGGAACACGCCGATCACACCGCCCAGGATCca < 1:3713450/62‑1 (MQ=255) aagaagaagaaCAGGAACAGGTCGATGGCGAGGAACACGCCGATCACACCGCCCAGGATCca < 1:4091278/62‑1 (MQ=255) aagaagaagaaCAGGAACAGGTCGATGGCGAGGAACACGCCGATCACACCGCCCAGGATCca < 1:2188976/62‑1 (MQ=255) gaagaagaaCAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGa > 1:5109751/1‑56 (MQ=255) gaagaaCAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATTCACATCAgg > 1:2747887/1‑64 (MQ=255) gaagaaCAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAgg > 1:2887115/1‑64 (MQ=255) gaagaaCAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAgg > 1:612069/1‑64 (MQ=255) gaagaaCAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAgg > 1:2136153/1‑64 (MQ=255) gaagaaCAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAgg > 1:2982546/1‑64 (MQ=255) gaagaaCAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAgg > 1:1752023/1‑64 (MQ=255) gaagaaCAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGt > 1:2288601/1‑71 (MQ=255) aagaaCAGGAACATGTCGATGGCAAGGAACACGCCGATAAcg < 1:1366484/42‑1 (MQ=255) agaaCAGGAACATGTCGATGGCAAGGAACACGCCGATAAcg < 1:2370373/41‑1 (MQ=255) agaaCAGGAACATGTCGATGGCAAGGAACACGCCGATAAcg < 1:2654791/41‑1 (MQ=255) aCATGTCGATGGCAAGGAACACGCCGATAAcgccgc > 1:332863/1‑36 (MQ=255) aCATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCca > 1:3236265/1‑46 (MQ=255) aCATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATc > 1:2050930/1‑50 (MQ=255) aCATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATc > 1:1544512/1‑50 (MQ=255) aCATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCa > 1:4016406/1‑51 (MQ=255) aCATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCa > 1:495916/1‑51 (MQ=255) aCATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCa > 1:2153989/1‑51 (MQ=255) aCATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCa > 1:4399548/1‑51 (MQ=255) aCATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCa > 1:1643898/1‑51 (MQ=255) aTGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTgg < 1:1334506/60‑1 (MQ=255) gTCGATGGCAAGGAACACGCCGATAACGCCGCCCAgg > 1:1296419/1‑37 (MQ=255) gTCGATGGCAAGGAACACGCCGATAACGCCGCCCAgg > 1:4944848/1‑37 (MQ=255) gTCGATGGCAAGGAACACGCCGATAACGCCGCCCAgg > 1:1745620/1‑37 (MQ=255) gTCGATGGCAAGGAACACGCCGATAACGCCGCCCAgg > 1:4543982/1‑37 (MQ=255) gTCGATGGCAAGGAACACGCCGATAACGCCGCCCAgg > 1:293323/1‑37 (MQ=255) gTCGATGGCAAGGAACACGCCGATAACGCCGCCCAgg > 1:4587768/1‑37 (MQ=255) gTCGATGGCAAGGAACACGCCGATAACGCCGCCCAgg > 1:1687650/1‑37 (MQ=255) gTCGATGGCAAGGAACACGCCGATAACGCCGCCCAgg > 1:166177/1‑37 (MQ=255) gTCGATGGCAAGGAACACGCCGATAACGCCGCCCAg > 1:3094202/1‑36 (MQ=255) gTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAgg < 1:3997151/49‑1 (MQ=255) gTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAgg < 1:1340821/49‑1 (MQ=255) gTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAgg < 1:4022782/49‑1 (MQ=255) gTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAgg < 1:5114574/49‑1 (MQ=255) gTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAgg < 1:1720408/49‑1 (MQ=255) gTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAg > 1:4506119/1‑54 (MQ=255) gTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAg > 1:1699321/1‑54 (MQ=255) gTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAg > 1:1526321/1‑54 (MQ=255) gTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAg > 1:1523374/1‑54 (MQ=255) tCGATGGCGAGGAACACGCCGATCACACCGCCCAGGATCCACAGCAGGTTCAGATGga > 1:3359346/1‑58 (MQ=255) tCGATGGCGAGGAACACGCCGATCACACCGCCCAGGATCCACAGCAGGTTCAGATGga > 1:4972047/1‑58 (MQ=255) tCGATGGCGAGGAACACGCCGATCACACCGCCCAGGATCCACAGCAGGTTCAGATGga > 1:4902287/1‑58 (MQ=255) tCGATGGCGAGGAACACGCCGATCACACCGCCCAGGATCCACAGCAGGTTCAGATGga > 1:1095425/1‑58 (MQ=255) tGGCAAGGAACACGCCGATAACGCCGCCCAGGATcc < 1:2641569/36‑1 (MQ=255) tGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAgg < 1:1258148/44‑1 (MQ=255) aaGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGa > 1:2522150/1‑71 (MQ=255) gAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGt < 1:2876952/44‑1 (MQ=255) gAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGt < 1:1343273/44‑1 (MQ=255) gAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGt < 1:4559179/44‑1 (MQ=255) aaCACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGAtctc > 1:1430015/1‑71 (MQ=255) aaCACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGAtctc > 1:4056653/1‑71 (MQ=255) aaCACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGAt > 1:435225/1‑68 (MQ=255) aaCACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGAt > 1:4000879/1‑68 (MQ=255) aaCACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGAt > 1:154742/1‑68 (MQ=255) aaCACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGAt > 1:891052/1‑68 (MQ=255) aaCACGCCGATAACGCCGCCCAGGATCCACATCAGGTT‑AGGTGGAAGAAGCCCTGATATTTTTCGATctct > 1:3489106/1‑71 (MQ=255) acacGCCGATAACGCCGCCCAGGATCCACATCAgg < 1:4350580/35‑1 (MQ=255) acacGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGGGGAAGAAGCCCTGATATTTTTCg < 1:3751305/65‑1 (MQ=255) acGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCttt < 1:3915376/71‑1 (MQ=255) acGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCttt < 1:3657517/71‑1 (MQ=255) acGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCttt < 1:4598837/71‑1 (MQ=255) cGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTc > 1:4636644/1‑71 (MQ=255) cGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTc > 1:2929432/1‑71 (MQ=255) cGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTc > 1:2551157/1‑71 (MQ=255) cGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTc > 1:2454803/1‑71 (MQ=255) cGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTc > 1:2228706/1‑71 (MQ=255) cGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCACttac > 1:181104/1‑69 (MQ=255) ccGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATAtttt > 1:1223194/1‑57 (MQ=255) cGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCAc < 1:3501276/71‑1 (MQ=255) aTAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGAtct < 1:75664/61‑1 (MQ=255) aTAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGAtct < 1:2669801/61‑1 (MQ=255) aTAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGAtct < 1:2660206/61‑1 (MQ=255) aTAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGAtct < 1:201660/61‑1 (MQ=255) aTAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACg > 1:3128120/1‑70 (MQ=255) aTAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACg > 1:700406/1‑70 (MQ=255) aTAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGa < 1:2762213/71‑1 (MQ=255) aTAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGa < 1:5189226/71‑1 (MQ=255) tAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGaa > 1:2494152/1‑71 (MQ=255) tAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGaa < 1:4813241/71‑1 (MQ=255) tAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGa > 1:1235916/1‑70 (MQ=255) tAACGCCGACCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGaa > 1:4397262/1‑71 (MQ=255) aaCGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGaa > 1:168476/1‑70 (MQ=255) aaCGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGaa > 1:2610114/1‑70 (MQ=255) aaCGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGaa > 1:3538139/1‑70 (MQ=255) aaCGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGaa > 1:4127212/1‑70 (MQ=255) | ATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGATGGCAAGGAACACGCCGATAACGCCGCCCAGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAA > minE/1488579‑1488716 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |