| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,488,719 | T→G | 10.0% | L103L (CTA→CTC) | nuoM ← | NADH:ubiquinone oxidoreductase, membrane subunit M |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,488,719 | 0 | T | G | 10.0% | 248.2 / 21.6 | 99 | L103L (CTA→CTC) | nuoM | NADH:ubiquinone oxidoreductase, membrane subunit M |
| Reads supporting (aligned to +/- strand): ref base T (70/19); new base G (4/6); total (74/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.53e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 103 total aligned reads displayed.
AGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGAT > minE/1488657‑1488789 | agcaTCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATACTACCGc < 1:2455536/68‑1 (MQ=255)aGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTAcc < 1:1105239/69‑1 (MQ=255)aGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTAcc < 1:324182/69‑1 (MQ=255)aGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTAcc < 1:3581336/69‑1 (MQ=255)aGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTAcc < 1:696209/69‑1 (MQ=255) gATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATAGt > 1:2451859/1‑64 (MQ=255) gATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGc > 1:2416326/1‑69 (MQ=255) gATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGc > 1:4696703/1‑69 (MQ=255) gATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGc > 1:4737967/1‑69 (MQ=255) gATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGc > 1:1545618/1‑69 (MQ=255) gATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGc > 1:3848426/1‑69 (MQ=255) tCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGCCAg > 1:1652934/1‑70 (MQ=255) tCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGCCAg < 1:1194506/70‑1 (MQ=255) tCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGCCAg > 1:4560682/1‑70 (MQ=255) tCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGCCAg > 1:4768977/1‑70 (MQ=255) tCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGCCAg > 1:4719017/1‑70 (MQ=255) tCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCAACGAACATAGTACCGCCa > 1:87844/1‑69 (MQ=255) acaTCAGGTTGAGGTGGAAGAAGGCCTGATATTTTTCGATCTCTTTCCACGAACAGAGTACCGccgg > 1:119239/1‑65 (MQ=255) acaTCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACAGAGTACCGCCAg > 1:504440/1‑67 (MQ=255) acaTCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACAGAGTACCGCCAg > 1:4808637/1‑67 (MQ=255) acaTCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACAGAGTACCGCCAg > 1:4725458/1‑67 (MQ=255) caTCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGCCAGCacac > 1:34939/1‑71 (MQ=255) caTCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGCCAGCacac > 1:869044/1‑71 (MQ=255) aGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAg > 1:4325456/1‑71 (MQ=255) ggTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGCCACCACACCGAGc < 1:464702/71‑1 (MQ=255) tGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCa > 1:2169873/1‑69 (MQ=255) tGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCa > 1:1054262/1‑69 (MQ=255) tGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCa > 1:3443263/1‑69 (MQ=255) tGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCa > 1:5073500/1‑69 (MQ=255) gAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATa > 1:135274/1‑47 (MQ=255) gAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATa > 1:2224119/1‑47 (MQ=255) gAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGAc > 1:2821955/1‑71 (MQ=255) gaagaaGCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGt > 1:4071143/1‑69 (MQ=255) agaagCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGCCAGCACACGGAGCAGACCGGTCAg > 1:3792873/1‑70 (MQ=255) agaagCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAg > 1:1417368/1‑58 (MQ=255) agaagCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAg > 1:3389323/1‑58 (MQ=255) agaagCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAg > 1:2180938/1‑58 (MQ=255) agaagCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAg > 1:702188/1‑58 (MQ=255) gCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGa < 1:1106812/71‑1 (MQ=255) ccTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGAc > 1:4641240/1‑70 (MQ=255) tGATATTTTTCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAg < 1:1832852/62‑1 (MQ=255) gATATTTTTCTATCTCTTTCCACGAACAGAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGAc < 1:1611153/67‑1 (MQ=255) gATATTTTTCTATCTCTTTCCACGAACAGAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGAc < 1:2968461/67‑1 (MQ=255) gATATTTTTCGATCTCTTTCCACGAACAGAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGAc < 1:2289720/67‑1 (MQ=255) gATATTTTTCGATCTCTTTCCACGAACAGAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGAc < 1:4478611/67‑1 (MQ=255) gATATTTTTCGATCTCTTTCCACGAACAGAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGAc < 1:1680038/67‑1 (MQ=255) gATATTTTTCGATCTCTTTCCACGAACAGAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGAc < 1:4588041/67‑1 (MQ=255) tatTTTTCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATcag > 1:3432250/1‑71 (MQ=255) atTTTTCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATcag > 1:2681099/1‑70 (MQ=255) tttttCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCa > 1:4672537/1‑59 (MQ=255) tttttCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATcag > 1:13005/1‑69 (MQ=255) tttttCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATcag > 1:2153947/1‑69 (MQ=255) tttttCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATcag > 1:1954598/1‑69 (MQ=255) tttttCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATcag > 1:4177999/1‑69 (MQ=255) tttttCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATcag > 1:4918578/1‑69 (MQ=255) tttttCGATCTCTTTCCACGAACATAGTACAGCCAGCACACCGAGCAGACCGGTCa > 1:1558065/1‑56 (MQ=255) tttCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCagcagc > 1:815723/1‑71 (MQ=255) ttCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCagcagc > 1:4401873/1‑70 (MQ=255) ttCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCagcagc > 1:2719397/1‑70 (MQ=255) tCGATCTCTTTCGACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCg < 1:4474548/70‑1 (MQ=255) tCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGa > 1:4982629/1‑45 (MQ=255) tCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGa > 1:592949/1‑45 (MQ=255) tCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGa > 1:842601/1‑45 (MQ=255) tCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGa > 1:1120763/1‑45 (MQ=255) tCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGa > 1:879114/1‑45 (MQ=255) tCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCg < 1:2731391/70‑1 (MQ=255) tCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCg < 1:2920931/70‑1 (MQ=255) tCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCg < 1:37006/70‑1 (MQ=255) tCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCg < 1:1356107/70‑1 (MQ=255) tCGATCTATTTCCACGAACATAGTACCGCCAGCACACCGAGCAGa > 1:3911443/1‑45 (MQ=255) tctTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGccc > 1:1323555/1‑71 (MQ=255) tctTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGccc > 1:4589345/1‑71 (MQ=255) ctTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCg > 1:315333/1‑71 (MQ=255) ttCCACGAACATAGTACTGCCAGCACACCGAGCAGACCGGTCAGCACGACCATcagcag > 1:4729546/1‑59 (MQ=255) ttCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCa > 1:969395/1‑46 (MQ=255) ttCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATcagcag > 1:2134388/1‑59 (MQ=255) ttCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATcagcag > 1:2058543/1‑59 (MQ=255) ttCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATcagcag > 1:2070248/1‑59 (MQ=255) ttCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATcagcag > 1:336271/1‑59 (MQ=255) ttCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATcagcag > 1:3169019/1‑59 (MQ=255) ttCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATcagcag > 1:3483060/1‑59 (MQ=255) ttCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATcagcag > 1:2351797/1‑59 (MQ=255) ttCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATcagcag > 1:3693314/1‑59 (MQ=255) ttCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATcagcag > 1:4130358/1‑59 (MQ=255) ttCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATcagcag > 1:2065212/1‑59 (MQ=255) tCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATcagca > 1:3917408/1‑57 (MQ=255) tCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATcagca > 1:843104/1‑57 (MQ=255) tCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATcagca > 1:5063782/1‑57 (MQ=255) tCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATcagca > 1:2442525/1‑57 (MQ=255) tCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTc < 1:1981334/70‑1 (MQ=255) tCCACGAAAATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATcagca > 1:3134759/1‑57 (MQ=255) cACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAAt > 1:3365362/1‑71 (MQ=255) cGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATgg < 1:2516882/71‑1 (MQ=255) cGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATgg < 1:3524152/71‑1 (MQ=255) cGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATgg < 1:909864/71‑1 (MQ=255) cGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATgg < 1:3364593/71‑1 (MQ=255) tAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGAt > 1:143480/1‑71 (MQ=255) tAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGAt > 1:714730/1‑71 (MQ=255) tAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGAt > 1:3516667/1‑71 (MQ=255) tAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGAt > 1:2055226/1‑71 (MQ=255) | AGGATCCACATCAGGTTGAGGTGGAAGAAGCCCTGATATTTTTCGATCTCTTTCCACGAACATAGTACCGCCAGCACACCGAGCAGACCGGTCAGCACGACCATCAGCAGCGACAGCCCGTCAATGGCGAGAT > minE/1488657‑1488789 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |