| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,515,674 | A→G | 7.8% | A114A (GCT→GCC) | cvpA ← | membrane protein required for colicin V production |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,515,674 | 0 | A | G | 7.8% | 302.5 / 11.9 | 102 | A114A (GCT→GCC) | cvpA | membrane protein required for colicin V production |
| Reads supporting (aligned to +/- strand): ref base A (45/49); new base G (2/6); total (47/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
Only 100 of 103 total aligned reads displayed.
GTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCC > minE/1515610‑1515742 | gtgATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGAAGcaac > 1:4181876/1‑70 (MQ=255) tgATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGcaac < 1:2099471/69‑1 (MQ=255) aTTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAAt > 1:3358004/1‑70 (MQ=255) aTTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAAt > 1:1171598/1‑70 (MQ=255) ttGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCGGCAACAATCaa > 1:2053290/1‑71 (MQ=255) ttGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCGGCAACAATCaa > 1:4165437/1‑71 (MQ=255) tGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCaa < 1:2327828/70‑1 (MQ=255) tGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCaa > 1:232326/1‑70 (MQ=255) tGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCaa < 1:2029814/70‑1 (MQ=255) tGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCaa > 1:866102/1‑70 (MQ=255) gCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCaa < 1:1102742/69‑1 (MQ=255) ccAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAacacac > 1:4396535/1‑71 (MQ=255) ccAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAacacac > 1:4780725/1‑71 (MQ=255) cAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACAcc > 1:246270/1‑71 (MQ=255) cAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACAcc > 1:177600/1‑71 (MQ=255) aGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACcg > 1:157232/1‑71 (MQ=255) ttCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACAcc < 1:4338244/66‑1 (MQ=255) ttCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCgcgc > 1:3324452/1‑70 (MQ=255) ttCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCgcgc > 1:2921515/1‑70 (MQ=255) ttCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCgcgc > 1:1959088/1‑70 (MQ=255) ttCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCgcgc > 1:5195688/1‑70 (MQ=255) ttttCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCGGCAACAATCAACACACcgc < 1:1965355/63‑1 (MQ=255) ttttCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCGGCAACAATCAACACACcgc < 1:342046/63‑1 (MQ=255) ttttCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCGGCAACAATCAACACACcgc < 1:3684561/63‑1 (MQ=255) ttttCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCGGCAACAATCAACACACcgc < 1:684391/63‑1 (MQ=255) ttttCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCGGCAACAATCAACACACcgc < 1:2152933/63‑1 (MQ=255) tttCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCGGCAACAATCAACACACcgc < 1:1487011/62‑1 (MQ=255) cGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCaa > 1:4343462/1‑63 (MQ=255) cGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCaa > 1:3410069/1‑63 (MQ=255) cGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCaa > 1:2489413/1‑63 (MQ=255) cGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCaa > 1:3968912/1‑63 (MQ=255) gACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGaa < 1:3946503/71‑1 (MQ=255) gACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGaa < 1:4940352/71‑1 (MQ=255) gACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGaa < 1:4401569/71‑1 (MQ=255) gACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGaa < 1:4351187/71‑1 (MQ=255) gACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGa < 1:989519/70‑1 (MQ=255) gACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGa < 1:4189676/70‑1 (MQ=255) gACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGa < 1:3016444/70‑1 (MQ=255) gACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGa < 1:1119980/70‑1 (MQ=255) gACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGa < 1:4546336/70‑1 (MQ=255) gACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGa < 1:1853889/70‑1 (MQ=255) gACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGa < 1:4825504/70‑1 (MQ=255) gACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGa < 1:5152027/70‑1 (MQ=255) gACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGa < 1:1179703/70‑1 (MQ=255) gACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGa < 1:1274628/70‑1 (MQ=255) gACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCCCGCAACGCACCGa < 1:1857907/70‑1 (MQ=255) acacCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGTCAGCAACAATCAACACACCGCGCAACGCACCGa < 1:829293/69‑1 (MQ=255) aaaGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGcc > 1:2799266/1‑69 (MQ=255) aaaGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGcc > 1:2097191/1‑69 (MQ=255) ggAGTCGAGAAAGAAGAGAATGGCAGCAACAATCaa > 1:3238062/1‑36 (MQ=255) ggAGTCGAGAAAGAAGAGAATGGCAGCAACAATCaa > 1:3703158/1‑36 (MQ=255) ggAGTCGAGAAAGAAGAGAATGGCAGCAACAATCaa > 1:3899929/1‑36 (MQ=255) ggAGTCGAGAAAGAAGAGAATGGCAGCAACAATCaa > 1:4938817/1‑36 (MQ=255) ggAGTCGAGAAAGAAGAGAATGGCAGCAACAATCaa > 1:4193534/1‑36 (MQ=255) ggAGTCGAGAAAGAAGAGAATGGCAGCAACAATCaa > 1:4463264/1‑36 (MQ=255) ggAGTCGAGAAAGAAGAGAATGGCAGCAACAATCaa > 1:140445/1‑36 (MQ=255) ggAGTCGAGAAAGAAGAGAATGGCAGCAACAATCaa > 1:319032/1‑36 (MQ=255) ggAGTCGAGAAAGAAGAGAATGGAAGCAACAATCaa > 1:1004200/1‑36 (MQ=255) tCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCgcgc < 1:3404376/42‑1 (MQ=255) tCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCgcgc < 1:106834/42‑1 (MQ=255) tCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCgcgc < 1:1411583/42‑1 (MQ=255) tCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCgcgc < 1:3217312/42‑1 (MQ=255) tCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCg < 1:4498374/71‑1 (MQ=255) gagaAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGAt > 1:792740/1‑71 (MQ=255) gagaAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGAt > 1:1497034/1‑71 (MQ=255) gagaAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGAt > 1:1592797/1‑71 (MQ=255) agaaagaaGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACa < 1:675245/53‑1 (MQ=255) agaaagaaGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACa < 1:1923791/53‑1 (MQ=255) agaaagaaGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATc < 1:3583042/71‑1 (MQ=255) agaaagaaGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATc < 1:300430/71‑1 (MQ=255) agaaagaaGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATc < 1:2681782/71‑1 (MQ=255) agaaagaaGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACCCCCAGCACCCGATc < 1:3893574/71‑1 (MQ=255) gaaagaaGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATc < 1:3560685/70‑1 (MQ=255) aaagaaGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGa > 1:931868/1‑53 (MQ=255) aaagaaGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGa > 1:200527/1‑53 (MQ=255) aaagaaGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGa > 1:3354156/1‑53 (MQ=255) aaagaaGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGa > 1:3021717/1‑53 (MQ=255) aaagaaGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGa > 1:2748435/1‑53 (MQ=255) aaagaaGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAg < 1:1152638/60‑1 (MQ=255) aaagaaGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAg < 1:948200/60‑1 (MQ=255) aaagaaGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAg < 1:4825943/60‑1 (MQ=255) aaagaaGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAg < 1:262280/60‑1 (MQ=255) aaagaaGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCgg > 1:4454878/1‑71 (MQ=255) aaagaaGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCgg > 1:4448652/1‑71 (MQ=255) aagaagAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAg < 1:2996800/59‑1 (MQ=255) gaagaGAATTGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGc < 1:205427/71‑1 (MQ=255) gaagaGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGc < 1:5023072/71‑1 (MQ=255) gaagaGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGc < 1:3008375/71‑1 (MQ=255) aagaGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGc < 1:4345039/70‑1 (MQ=255) agagAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCt < 1:780759/71‑1 (MQ=255) gagaATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTg < 1:3171966/71‑1 (MQ=255) gaATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCt < 1:4253633/68‑1 (MQ=255) gaATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCt < 1:1076415/68‑1 (MQ=255) gaATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCt < 1:1554338/68‑1 (MQ=255) gaATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGa > 1:1641915/1‑70 (MQ=255) gaATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGa > 1:2579215/1‑70 (MQ=255) gaATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGAc < 1:3238440/71‑1 (MQ=255) gaATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGAc < 1:3804840/71‑1 (MQ=255) gaAAGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACa > 1:2477616/1‑43 (MQ=255) cagcaACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAAccc > 1:314271/1‑70 (MQ=255) | GTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGAAAGAAGAGAATGGCAGCAACAATCAACACACCGCGCAACGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCC > minE/1515610‑1515742 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |