| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 432,031:1 | +T | 6.7% | coding (397/1407 nt) | ybfM → | predicted outer membrane porin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 432,031 | 1 | . | T | 6.7% | 225.5 / 13.9 | 60 | coding (397/1407 nt) | ybfM | predicted outer membrane porin |
| Reads supporting (aligned to +/- strand): ref base . (22/34); new base T (2/2); total (24/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01 | |||||||||||
CGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑TGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAGGGCAGGTTACATTCAGCC > minE/431961‑432095 | cgaaATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGcc > 1:2332484/1‑71 (MQ=255) gaaATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑t < 1:2324288/71‑1 (MQ=255) aaTCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑Tg > 1:2737545/1‑70 (MQ=255) gcgTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑TGTATaa < 1:1235869/71‑1 (MQ=255) ttttCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑Tg < 1:2104448/63‑1 (MQ=255) ttttCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑Tg < 1:3360568/63‑1 (MQ=255) tttCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑TGTATAAAGCt > 1:2731017/1‑71 (MQ=255) tttCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑TGTATAAAGCt > 1:2944055/1‑71 (MQ=255) ttCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑TGTATaa > 1:876417/1‑66 (MQ=255) ttCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑TGTATAAAGCt < 1:1909196/70‑1 (MQ=255) tCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑TTTATAAAGCTg < 1:2414328/70‑1 (MQ=255) tCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑TGTATAAAGCTg < 1:3701358/70‑1 (MQ=255) tCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑TGTATAAAGCTg < 1:3277109/70‑1 (MQ=255) tCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑TGTATAAAGCTg < 1:2351553/70‑1 (MQ=255) tCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑TGTATAAAGCTg < 1:874656/70‑1 (MQ=255) tCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑TGTATAAAGCTg < 1:690346/70‑1 (MQ=255) tCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑TGTATAAAGCTGc > 1:3119130/1‑71 (MQ=255) aaaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑TGTATAAAGCTGCgg > 1:214251/1‑69 (MQ=255) aaGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑TGTATAAAg > 1:2259662/1‑62 (MQ=255) aGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑TGTATAAAGCTGCGGCCaa < 1:438937/71‑1 (MQ=255) taaAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑TGTATAAAGCTGCGGCCAAATTTa < 1:3587885/71‑1 (MQ=255) aaaGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑TGTATAAAg > 1:2919235/1‑55 (MQ=255) aTGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑TGTATAAAGCTGCGGCCAAATTTaaa < 1:2424114/65‑1 (MQ=255) tGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑TGTATAAAGCTGCGGc < 1:504535/54‑1 (MQ=255) tGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑TGTATAAAGCTGCGGc < 1:402322/54‑1 (MQ=255) tGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑TGTATAAAGCTGCGGc < 1:3535114/54‑1 (MQ=255) tGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑TGTATAAAGCTGCGGc < 1:1156006/54‑1 (MQ=255) tGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑TGTATAAAGCTGCGGc < 1:1852606/54‑1 (MQ=255) gACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑t > 1:574879/1‑38 (MQ=255) aGACTGGTCCGGCGACAAAAGCGGTATAAGCCTTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGtt < 1:335641/71‑1 (MQ=255) aGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑TGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGttt < 1:3059203/71‑1 (MQ=255) aGACTAGTCCGGCGACAAAAGCGGTATAAGCCTTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGtt < 1:1328061/71‑1 (MQ=255) tGGTCCGGCGACAAAAGCGGTATAAGCC‑TGTATAAAGCTGCGGCCAAAt > 1:1972271/1‑49 (MQ=255) tGGTCCGGCGACAAAAGCGGTATAAGCC‑TGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGc > 1:3239222/1‑71 (MQ=255) gTCCGGCGACAAAAGCGGTATAAGCCTTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGc > 1:3295594/1‑70 (MQ=255) gTCCGGCGACAAAAGCGGTATAAGCCTTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGc > 1:3291091/1‑70 (MQ=255) gTCCGGCGACAAAAGCGGTATAAGCC‑TGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGc > 1:3344938/1‑69 (MQ=255) gTCCGGCGACAAAAGCGGTATAAGCC‑TGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGc > 1:3349202/1‑69 (MQ=255) gTCCGGCGACAAAAGCGGTATAAGCC‑TGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCg < 1:2533132/70‑1 (MQ=255) ccGGCGACAAAAGCGGTATAAGCC‑TGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAgg > 1:2324528/1‑71 (MQ=255) cGGCGACAAAAGCGGTATAAGCC‑TGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAggg < 1:2998378/71‑1 (MQ=255) ggCGACAAAAGCGGTATAATCC‑TGTATAAAGCTGCGGCCAAAtt < 1:2913294/44‑1 (MQ=255) ggCGACAAAAGCGGTATAAGCC‑TGTATAAAGCTGCGGCCAAAtt < 1:3444406/44‑1 (MQ=255) ggCGACAAAAGCGGTATAAGCC‑TGTATAAAGCTGCGGCCAAAtt < 1:1019666/44‑1 (MQ=255) ggCGACAAAAGCGGTATAAGCC‑TGTATAAAGCTGCGGCCAAAtt < 1:735620/44‑1 (MQ=255) aaaaGCGGTATAAGCC‑TGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGt > 1:1950797/1‑53 (MQ=255) aaaaGCGGTATAAGCC‑TGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGt > 1:2577400/1‑53 (MQ=255) aaaaGCGGTATAAGCC‑TGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGt > 1:2086112/1‑53 (MQ=255) aaaaGCGGTATAAGCC‑TGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAGGGCAGGTTa < 1:2990425/71‑1 (MQ=255) aaaaGCGGTATAAGCC‑TGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAGGGCAGGTTa > 1:3805328/1‑71 (MQ=255) aaGCGGTATAAGCC‑TGTATAAAGCTGCGGCCAAATTTaaa < 1:1505799/40‑1 (MQ=255) aaGCGGTATAAGCC‑TGTATAAAGCTGCGGCCAAATTTaaa < 1:2070583/40‑1 (MQ=255) aGCGGTATAAGCC‑TGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAGGGCAGGTTACAt > 1:297889/1‑71 (MQ=255) aGCGGTATAAGCC‑TGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAGGGCAGGTTACAt > 1:1613362/1‑71 (MQ=255) gCGGTATAAGCC‑TGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGTTTTGGGCGAGGGCAGGTTACAtt < 1:2004268/71‑1 (MQ=255) gCGGTATAAGCC‑TGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAGGGCAGGTTACAtt < 1:3536073/71‑1 (MQ=255) cGGTATAAGCC‑TGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAGGGCAGGTTACATTc < 1:1213000/71‑1 (MQ=255) taATCC‑TGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGATTTGGCGAGGGCAGGTAACATTCAgag > 1:1983285/1‑68 (MQ=255) taAGCC‑TGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAGGGCAGGTTAc < 1:1988752/62‑1 (MQ=255) taAGCC‑TGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAGGGCAGGTTACATTCa > 1:3647158/1‑67 (MQ=255) taAGCC‑TGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAGGGCAGGTTACATTCAGcc > 1:3494661/1‑70 (MQ=255) taAGCC‑TGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAGGGCAGGTTACATTCAGcc < 1:2122608/70‑1 (MQ=255) taAGCC‑TGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAGGGCAGGTTACATTCAGcc < 1:2713633/70‑1 (MQ=255) c‑TGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAGGGCAGGTTACATTc > 1:2645334/1‑61 (MQ=255) | CGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCC‑TGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAGGGCAGGTTACATTCAGCC > minE/431961‑432095 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |