| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 323,687 | T→C | 7.7% | R33R (CGT→CGC) | htpG → | molecular chaperone HSP90 family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 323,687 | 0 | T | C | 7.7% | 186.2 / 6.9 | 65 | R33R (CGT→CGC) | htpG | molecular chaperone HSP90 family |
| Reads supporting (aligned to +/- strand): ref base T (31/29); new base C (3/2); total (34/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.77e-01 | |||||||||||
TCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTG > minE/323619‑323756 | tcagAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGa < 1:816421/71‑1 (MQ=255)tcagAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGa < 1:3861034/71‑1 (MQ=255) agAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGc > 1:2990543/1‑71 (MQ=255) aaGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCtt < 1:2270439/71‑1 (MQ=255) aaGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCt > 1:1903994/1‑70 (MQ=255) aGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTa < 1:3550891/71‑1 (MQ=255) aGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATACCAATAAAGAAATCTTCCTGCGTGAGCTTa < 1:32092/71‑1 (MQ=255) gTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTg < 1:2645261/64‑1 (MQ=255) aaaCAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTAtctc > 1:3263640/1‑71 (MQ=255) aaCAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATctct < 1:2622752/71‑1 (MQ=255) aCAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTa < 1:3053736/71‑1 (MQ=255) aGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAAc > 1:2346884/1‑71 (MQ=255) ttCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGcc > 1:3372737/1‑71 (MQ=255) tCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCt > 1:3089595/1‑71 (MQ=255) cTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTc > 1:2029569/1‑71 (MQ=255) cTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTc > 1:2425837/1‑71 (MQ=255) gCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATctct > 1:3042005/1‑61 (MQ=255) gCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATctct > 1:2294259/1‑61 (MQ=255) gCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATctct > 1:2331874/1‑61 (MQ=255) cACCTGATGATTCATTCTCTCTATTCGAACAAGGAAATCTTCCTCCGCGAACTGAtttcc < 1:352644/60‑2 (MQ=255) cACCTGATGATTCATTCTCTCTATTCGAACAAGGAAATCTTCCTCCGCGAACTGAtttcc < 1:578528/60‑2 (MQ=255) tgatgaTCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATctct < 1:2318751/56‑1 (MQ=255) tgatgaTCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTcc < 1:329765/65‑1 (MQ=255) gatgatCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATctct < 1:3105474/55‑1 (MQ=255) atgatCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATgcggc > 1:1450887/1‑71 (MQ=255) atgatCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATgcggc > 1:182401/1‑71 (MQ=255) atgatCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATgcg < 1:559793/69‑1 (MQ=255) tgatTCATTCTCTCTATTCGAACAAGGAAATCTTCCTCCGCGAACTGATTTCCAACGCCTCGGAcgccgcc > 1:1492779/1‑70 (MQ=255) tgatTCATTCTCTCTATTCGAACAAGGAAATCTTCCTCCGCGAACTGATTTCCAACGCCTCGGAcgccgcc > 1:1239532/1‑70 (MQ=255) tgatTCATTCTCTCTATTCGAACAAGGAAATCTTCCTCCGCGAACTGATTTCCAACGCCTCGGAcgccgcc > 1:3132299/1‑70 (MQ=255) tgatCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTAt > 1:1109411/1‑49 (MQ=255) tgatCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTAt > 1:2667968/1‑49 (MQ=255) atCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGcggcgg < 1:789668/70‑1 (MQ=255) tCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGAc < 1:3252897/71‑1 (MQ=255) tCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGAc < 1:2694386/71‑1 (MQ=255) cATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACa < 1:2168675/70‑1 (MQ=255) ttCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGc < 1:2886575/71‑1 (MQ=255) ctctctATTCCAATAAAGAAATCTTCCTGCGTGAGCTTAt > 1:2573957/1‑40 (MQ=255) tctATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGt > 1:3346866/1‑71 (MQ=255) tctATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGt > 1:3369438/1‑71 (MQ=255) ctATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGtt > 1:300829/1‑71 (MQ=255) aTTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGa < 1:778748/58‑1 (MQ=255) ttCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGa < 1:1535752/57‑1 (MQ=255) ttCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTcc < 1:754836/71‑1 (MQ=255) ccAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCg > 1:706672/1‑64 (MQ=255) aTAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATgcggcg > 1:1476747/1‑50 (MQ=255) aTAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGa > 1:3017473/1‑52 (MQ=255) aTAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGa > 1:550939/1‑52 (MQ=255) aTAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGa > 1:2623514/1‑52 (MQ=255) aTAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTc < 1:2891562/65‑1 (MQ=255) aTAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTgcg > 1:917190/1‑71 (MQ=255) aTAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTgcg > 1:3418423/1‑71 (MQ=255) aTAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTgcg > 1:2467666/1‑71 (MQ=255) tAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAg < 1:402544/55‑1 (MQ=255) tAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAg < 1:2636582/55‑1 (MQ=255) aaGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGct < 1:3815293/70‑1 (MQ=255) aaaTCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGttt < 1:419506/58‑1 (MQ=255) aaaTCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGctctc > 1:2671439/1‑70 (MQ=255) aaTCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTa < 1:152315/71‑1 (MQ=255) aaTCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTa < 1:1230648/71‑1 (MQ=255) ttCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACa < 1:2600311/43‑1 (MQ=255) ccTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAg > 1:2763397/1‑43 (MQ=255) ccTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCg < 1:3457111/70‑1 (MQ=255) gCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGAcc > 1:1734194/1‑71 (MQ=255) cGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCt > 1:3146981/1‑71 (MQ=255) tGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTg < 1:2117410/70‑1 (MQ=255) | TCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTG > minE/323619‑323756 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |