Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 323,690 G→A 7.9% E34E (GAG→GAA htpG → molecular chaperone HSP90 family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE323,6900GA 7.9% 198.2 / 6.4 63E34E (GAG→GAAhtpGmolecular chaperone HSP90 family
Reads supporting (aligned to +/- strand):  ref base G (31/27);  new base A (3/2);  total (34/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

AGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTG  >  minE/323621‑323756
                                                                     |                                                                  
agAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGc                                                                   >  1:2990543/1‑71 (MQ=255)
  aaGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCtt                                                                 <  1:2270439/71‑1 (MQ=255)
  aaGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCt                                                                  >  1:1903994/1‑70 (MQ=255)
   aGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTa                                                                <  1:3550891/71‑1 (MQ=255)
   aGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATACCAATAAAGAAATCTTCCTGCGTGAGCTTa                                                                <  1:32092/71‑1 (MQ=255)
       aaaCAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTAtctc                                                            >  1:3263640/1‑71 (MQ=255)
        aaCAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATctct                                                           <  1:2622752/71‑1 (MQ=255)
         aCAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTa                                                          <  1:3053736/71‑1 (MQ=255)
           aGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAAc                                                        >  1:2346884/1‑71 (MQ=255)
              ttCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGcc                                                     >  1:3372737/1‑71 (MQ=255)
               tCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCt                                                    >  1:3089595/1‑71 (MQ=255)
                cTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTc                                                   >  1:2029569/1‑71 (MQ=255)
                cTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTc                                                   >  1:2425837/1‑71 (MQ=255)
                  gCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATctct                                                           >  1:3042005/1‑61 (MQ=255)
                  gCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATctct                                                           >  1:2294259/1‑61 (MQ=255)
                  gCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATctct                                                           >  1:2331874/1‑61 (MQ=255)
                   cACCTGATGATTCATTCTCTCTATTCGAACAAGGAAATCTTCCTCCGCGAACTGAtttcc                                                           <  1:352644/60‑2 (MQ=255)
                   cACCTGATGATTCATTCTCTCTATTCGAACAAGGAAATCTTCCTCCGCGAACTGAtttcc                                                           <  1:578528/60‑2 (MQ=255)
                       tgatgaTCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATctct                                                           <  1:2318751/56‑1 (MQ=255)
                       tgatgaTCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTcc                                                  <  1:329765/65‑1 (MQ=255)
                        gatgatCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATctct                                                           <  1:3105474/55‑1 (MQ=255)
                         atgatCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATgcggc                                          >  1:1450887/1‑71 (MQ=255)
                         atgatCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATgcggc                                          >  1:182401/1‑71 (MQ=255)
                         atgatCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATgcg                                            <  1:559793/69‑1 (MQ=255)
                          tgatTCATTCTCTCTATTCGAACAAGGAAATCTTCCTCCGCGAACTGATTTCCAACGCCTCGGAcgccgcc                                         >  1:1492779/1‑70 (MQ=255)
                          tgatTCATTCTCTCTATTCGAACAAGGAAATCTTCCTCCGCGAACTGATTTCCAACGCCTCGGAcgccgcc                                         >  1:1239532/1‑70 (MQ=255)
                          tgatTCATTCTCTCTATTCGAACAAGGAAATCTTCCTCCGCGAACTGATTTCCAACGCCTCGGAcgccgcc                                         >  1:3132299/1‑70 (MQ=255)
                          tgatCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTAt                                                               >  1:1109411/1‑49 (MQ=255)
                          tgatCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTAt                                                               >  1:2667968/1‑49 (MQ=255)
                            atCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGcggcgg                                        <  1:789668/70‑1 (MQ=255)
                             tCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGAc                                      <  1:2694386/71‑1 (MQ=255)
                             tCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGAc                                      <  1:3252897/71‑1 (MQ=255)
                               cATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACa                                     <  1:2168675/70‑1 (MQ=255)
                                 ttCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGc                                  <  1:2886575/71‑1 (MQ=255)
                                   ctctctATTCCAATAAAGAAATCTTCCTGCGTGAGCTTAt                                                               >  1:2573957/1‑40 (MQ=255)
                                      tctATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGt                             >  1:3369438/1‑71 (MQ=255)
                                      tctATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGt                             >  1:3346866/1‑71 (MQ=255)
                                       ctATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGtt                            >  1:300829/1‑71 (MQ=255)
                                         aTTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGa                                       <  1:778748/58‑1 (MQ=255)
                                          ttCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGa                                       <  1:1535752/57‑1 (MQ=255)
                                          ttCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTcc                         <  1:754836/71‑1 (MQ=255)
                                            ccAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCg                              >  1:706672/1‑64 (MQ=255)
                                               aTAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATgcggcg                                         >  1:1476747/1‑50 (MQ=255)
                                               aTAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGa                                       >  1:550939/1‑52 (MQ=255)
                                               aTAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGa                                       >  1:2623514/1‑52 (MQ=255)
                                               aTAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGa                                       >  1:3017473/1‑52 (MQ=255)
                                               aTAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTc                          <  1:2891562/65‑1 (MQ=255)
                                               aTAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTgcg                    >  1:917190/1‑71 (MQ=255)
                                               aTAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTgcg                    >  1:3418423/1‑71 (MQ=255)
                                               aTAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTgcg                    >  1:2467666/1‑71 (MQ=255)
                                                tAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAg                                   <  1:402544/55‑1 (MQ=255)
                                                tAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAg                                   <  1:2636582/55‑1 (MQ=255)
                                                  aaGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGct                  <  1:3815293/70‑1 (MQ=255)
                                                     aaaTCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGttt                           <  1:419506/58‑1 (MQ=255)
                                                     aaaTCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGctctc               >  1:2671439/1‑70 (MQ=255)
                                                      aaTCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTa             <  1:152315/71‑1 (MQ=255)
                                                      aaTCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTa             <  1:1230648/71‑1 (MQ=255)
                                                          ttCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACa                                     <  1:2600311/43‑1 (MQ=255)
                                                            ccTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAg                                   >  1:2763397/1‑43 (MQ=255)
                                                            ccTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCg        <  1:3457111/70‑1 (MQ=255)
                                                               gCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGAcc    >  1:1734194/1‑71 (MQ=255)
                                                                cGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCt   >  1:3146981/1‑71 (MQ=255)
                                                                  tGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTg  <  1:2117410/70‑1 (MQ=255)
                                                                     |                                                                  
AGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTG  >  minE/323621‑323756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: