| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 894,873 | C→T | 5.5% | E398K (GAA→AAA) | sapA ← | predicted antimicrobial peptide transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 894,873 | 0 | C | T | 5.5% | 243.3 / 2.7 | 72 | E398K (GAA→AAA) | sapA | predicted antimicrobial peptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (32/36); new base T (2/2); total (34/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
GAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTT > minE/894804‑894943 | gAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCg > 1:1407785/1‑71 (MQ=255) aaaGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCgg > 1:1078706/1‑71 (MQ=255) aaaGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCgg < 1:891540/71‑1 (MQ=255) aaaGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCgg > 1:864189/1‑71 (MQ=255) aaaGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCgg > 1:972216/1‑71 (MQ=255) gACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGtt < 1:38721/71‑1 (MQ=255) aCCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGtt < 1:2138786/70‑1 (MQ=255) ccTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTTGGCAGtttt > 1:1312544/1‑71 (MQ=255) ccTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGtttt < 1:2133403/71‑1 (MQ=255) cTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTc < 1:2200253/71‑1 (MQ=255) cTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTc < 1:2586021/71‑1 (MQ=255) cTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTc < 1:2682694/71‑1 (MQ=255) cTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTc < 1:1752008/71‑1 (MQ=255) tCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGtt < 1:3601928/66‑1 (MQ=255) tCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGtt < 1:1831274/66‑1 (MQ=255) cTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCa < 1:1596790/69‑1 (MQ=255) cTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCa < 1:3253237/69‑1 (MQ=255) tACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGtt < 1:2562322/64‑1 (MQ=255) tACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCa < 1:1798989/68‑1 (MQ=255) tACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTg > 1:3671287/1‑71 (MQ=255) ccGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTg < 1:2185743/69‑1 (MQ=255) acaATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTggg > 1:1712019/1‑71 (MQ=255) ctCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAg < 1:1377424/58‑1 (MQ=255) aTCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAg < 1:2115434/59‑1 (MQ=255) aTCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAg < 1:3313818/59‑1 (MQ=255) aTCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAg < 1:2799399/59‑1 (MQ=255) aTCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGt > 1:3130319/1‑60 (MQ=255) aTCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGt > 1:13864/1‑60 (MQ=255) aTCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGt > 1:1302124/1‑60 (MQ=255) aTCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGt > 1:1766184/1‑60 (MQ=255) aTCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGt > 1:1635194/1‑60 (MQ=255) tCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGtttt < 1:2437188/55‑1 (MQ=255) tCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGtttt < 1:920641/55‑1 (MQ=255) tCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGtttt < 1:832555/55‑1 (MQ=255) tCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGtttt < 1:2002974/55‑1 (MQ=255) caccacTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAg < 1:2113932/57‑1 (MQ=255) caccacTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGtt > 1:1779428/1‑69 (MQ=255) accacTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTcc < 1:746007/70‑1 (MQ=255) acTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTTGGCAGTTTTCAGTGGACTGGGGTTCCACGc > 1:1299016/1‑71 (MQ=255) cTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGtttt < 1:3471637/49‑1 (MQ=255) cTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGtttt < 1:3564933/49‑1 (MQ=255) cTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGtttt < 1:3603868/49‑1 (MQ=255) cTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGtttt < 1:878177/49‑1 (MQ=255) cTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGtttt < 1:2149249/49‑1 (MQ=255) cTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGcc > 1:2402544/1‑71 (MQ=255) ttttACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCa < 1:1949774/50‑1 (MQ=255) ttttACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCt > 1:1153066/1‑71 (MQ=255) ttACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGt > 1:2976899/1‑43 (MQ=255) ttACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGc > 1:731686/1‑71 (MQ=255) ttACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGc > 1:423516/1‑71 (MQ=255) ttACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGc > 1:3463486/1‑71 (MQ=255) ttACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGc > 1:1516031/1‑71 (MQ=255) ttACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGc > 1:1611831/1‑71 (MQ=255) tACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGtttt < 1:956050/45‑1 (MQ=255) tACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCa > 1:1084059/1‑47 (MQ=255) aCGCCAACCTGCGCCATATCCGCCTGAATCAGTTTGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCg < 1:1042125/70‑1 (MQ=255) aCGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCg > 1:230448/1‑70 (MQ=255) aCGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCg > 1:1394646/1‑70 (MQ=255) gCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAAc < 1:2888965/71‑1 (MQ=255) aaCCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACgtg < 1:436741/71‑1 (MQ=255) aaCCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTCTTCAGTGGACTGGGGTTCCACGCCTGCGAACgtg > 1:358924/1‑71 (MQ=255) aCCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACgtgt > 1:1220175/1‑71 (MQ=255) cTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTgg > 1:2980539/1‑71 (MQ=255) cgcCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCa > 1:627020/1‑71 (MQ=255) ccATATCCGCCTGAATCAGTTTGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCAc < 1:565219/70‑1 (MQ=255) atCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACgtg < 1:1063284/59‑1 (MQ=255) tCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAg > 1:2414209/1‑71 (MQ=255) tCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAg > 1:214128/1‑71 (MQ=255) cGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTgg > 1:3526737/1‑59 (MQ=255) cGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTgg > 1:984247/1‑59 (MQ=255) cGCCTGAATCAGTCCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGtt > 1:211865/1‑71 (MQ=255) aGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTa < 1:2379289/70‑1 (MQ=255) cGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAAttt > 1:261688/1‑71 (MQ=255) | GAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTT > minE/894804‑894943 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |