| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 89,548 | A→G | 13.6% | E276G (GAA→GGA) | ftsA → | ATP‑binding cell division protein involved in recruitment of FtsK to Z ring |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 89,548 | 0 | A | G | 13.6% | 218.1 / 23.4 | 81 | E276G (GAA→GGA) | ftsA | ATP‑binding cell division protein involved in recruitment of FtsK to Z ring |
| Reads supporting (aligned to +/- strand): ref base A (34/36); new base G (4/7); total (38/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCCGAAGCGATTAAAGTTCGCCACGGTTGTGCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGTCAGACACTGGCAGAGGTGATCGAGCC > minE/89480‑89617 | cGCCGAAGCGATTAAAGTTCGCCACGGTTGTGCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGAAg < 1:620400/71‑1 (MQ=255) gCCGAAGCGATTAAAGTTCGCCACGGTTGTGCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGGAGt > 1:3732611/1‑71 (MQ=255) gCCGAAGCGATTAAAGTTCGCCACGGTTGTGCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGAAGt > 1:165785/1‑71 (MQ=255) cGAAGCGATTAAAGTTCGCCACGGTTGTGCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGGAGTGc > 1:286375/1‑71 (MQ=255) cGAAGCGATTAAAGTTCGCCACGGTTGTGCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGAAGTGc > 1:2053555/1‑71 (MQ=255) cGAAGCGATTAAAGTTCGCCACGGTTGTGCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGAAGTGc > 1:3391404/1‑71 (MQ=255) cGAAGCGATTAAAGTTCGCCACGGTTGTGCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGAAGTGc > 1:2939742/1‑71 (MQ=255) gAAGCGATTAAAGTTCGCCACGGTTGTGCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGAAGTGcc < 1:1684126/71‑1 (MQ=255) gAAGCGATTAAAGTTCGCCACGGTTGTGCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGAAGTGcc < 1:3604008/71‑1 (MQ=255) aaGCGATTAAAGTTCGCCACGGTTGTGCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGAAGTGcc < 1:1609403/70‑1 (MQ=255) tAAAGTTCGCCACGGTTGTGCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAg > 1:3389343/1‑71 (MQ=255) gTTCGCCACGGTTGTGCGCTGGGTTCCATCGTTGGAAAAGATTAGAGCGTGGGAGTGCCGAGCGTAggtgg < 1:3554565/71‑1 (MQ=255) gTTCGCCACGGTTGTGCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGGAGTGCCGAGCGTAggtgg > 1:3428191/1‑71 (MQ=255) gTTCGCCACGGTTGTGCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGGAGTGCCGAGCGTAggtgg < 1:2739196/71‑1 (MQ=255) gTTCGCCACGGTTGTGCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGGAGTGCCGAGCGTAggtgg > 1:2203804/1‑71 (MQ=255) gTTCGCCACGGTTGTGCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGGAGTGCCGAGCGTAggtgg < 1:1874898/71‑1 (MQ=255) gTTCGCCACGGTTGTGCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGGAGTGCCGAGCGTAggtgg < 1:1200900/71‑1 (MQ=255) gTTCGCCACGGTTGTGCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGGAGTGCCGAGCGTAggtgg < 1:3645248/71‑1 (MQ=255) gTTCGCCACGGTTGTGCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGGAGTGCCGAGCGTAggtgg < 1:778049/71‑1 (MQ=255) ttCGCCACGGTTGTGCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAggtggt > 1:977371/1‑71 (MQ=255) ttCGCCACGGTTGTGCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAggtgg > 1:1951577/1‑70 (MQ=255) tCGCCACGGTTGTGCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGa < 1:1376868/51‑1 (MQ=255) cGCCACGGTTGTGCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGgtc < 1:1127294/70‑1 (MQ=255) aCGGTTGTGCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCg > 1:3778896/1‑71 (MQ=255) ggTTGTGCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGcc < 1:918048/71‑1 (MQ=255) gTTGTGCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCa < 1:1426665/71‑1 (MQ=255) gtgCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACg < 1:2923219/70‑1 (MQ=255) gtgCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACg < 1:443590/70‑1 (MQ=255) tgCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACg < 1:2062765/69‑1 (MQ=255) tgCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACg < 1:1382498/69‑1 (MQ=255) gcgcTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAg > 1:1290186/1‑71 (MQ=255) gcgcTGGGTTCCATCGTTGGAAAAGATGAGAGCGAGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAg > 1:55722/1‑71 (MQ=255) gcTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTc > 1:2611066/1‑71 (MQ=255) cTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCt > 1:1185074/1‑71 (MQ=255) tGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTg > 1:1621455/1‑71 (MQ=255) ggTTCCATCGTTGGAAAAGATGAGAGCGTGGGAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGc < 1:2539353/70‑1 (MQ=255) ggTTCCATCGTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTg < 1:2406200/69‑1 (MQ=255) ggTTCCATCGTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGc > 1:473422/1‑70 (MQ=255) ggTTCCATCGTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGc < 1:572187/70‑1 (MQ=255) ttCCATCGTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAAc > 1:2465676/1‑71 (MQ=255) ccATCGTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACg > 1:226678/1‑70 (MQ=255) ccATCGTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGt > 1:378295/1‑71 (MQ=255) ccATCGTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGt > 1:3020789/1‑71 (MQ=255) ccATCGTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGt > 1:565190/1‑71 (MQ=255) ccATCGTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGt > 1:1606269/1‑71 (MQ=255) aTCGTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGTCa < 1:630886/71‑1 (MQ=255) gTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACg < 1:958333/53‑1 (MQ=255) gTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACg < 1:352544/53‑1 (MQ=255) tGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGTCAGaca > 1:424915/1‑70 (MQ=255) ggAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGTCAGACACt > 1:740274/1‑71 (MQ=255) aaaaGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACg < 1:2244737/48‑1 (MQ=255) aaaaGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACg < 1:1200342/48‑1 (MQ=255) aaaaGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACg < 1:839989/48‑1 (MQ=255) aaaaGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACg < 1:1281775/48‑1 (MQ=255) aaaaGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACg < 1:3193690/48‑1 (MQ=255) aaGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGTCAGACACTgg > 1:1280136/1‑69 (MQ=255) aaGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGTCAGACACTgg > 1:3339364/1‑69 (MQ=255) aTGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGTCAGACACt < 1:3550031/64‑1 (MQ=255) aTGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGTCAGACACt < 1:3578826/64‑1 (MQ=255) tGAGAGCGTGGAAGTTCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGTCAGACACTGGCAGAgg < 1:3623093/71‑1 (MQ=255) gagaGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACg > 1:1570650/1‑53 (MQ=255) gagaGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACg > 1:1359800/1‑53 (MQ=255) gagaGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACg > 1:758341/1‑53 (MQ=255) gagaGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACg > 1:2361395/1‑53 (MQ=255) agagCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGTCAGACACTGGCAGAGGt < 1:1630852/70‑1 (MQ=255) agagCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGTCAGACACTGGCAGAGGTg < 1:2341679/71‑1 (MQ=255) gagCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACg < 1:2299682/39‑1 (MQ=255) gagCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACg < 1:2585489/39‑1 (MQ=255) agCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACg < 1:2090343/38‑1 (MQ=255) gCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGTCAGACACTGGCAGAGGTGATc > 1:2734022/1‑71 (MQ=255) cGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGTCAGACACTGGCAGAGGTGATCg < 1:1057839/71‑1 (MQ=255) tGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGTCAGACACTGGCAGAGGt < 1:640310/64‑1 (MQ=255) tGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGTCAGACACTGGCAGAGGt < 1:1234247/64‑1 (MQ=255) tGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGTCAGACACTGGCAGAGGt < 1:3559654/64‑1 (MQ=255) tGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGTCAGACACTGGCAGAGGt < 1:2203790/64‑1 (MQ=255) tGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGTCAGACACTGGCAGAGGt < 1:1871913/64‑1 (MQ=255) gAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGTCAGACACTGGCAGAGGTGa > 1:3503731/1‑64 (MQ=255) gAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGTCAGACACTGGCAGAGGTGATCGAGcc > 1:3818075/1‑71 (MQ=255) gAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGTCAGACACTGGCAGAGGTGATCGAGcc > 1:1109212/1‑71 (MQ=255) gAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGTCAGACACTGGCAGAGGTGATCGAGcc > 1:1309727/1‑71 (MQ=255) gAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGTCAGACACTGGCAGAGGTGATCGAGc < 1:482324/70‑1 (MQ=255) gAAGTGACGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGTCAGACACTGGCAGAGGTGATCGAGcc > 1:525980/1‑71 (MQ=255) | CGCCGAAGCGATTAAAGTTCGCCACGGTTGTGCGCTGGGTTCCATCGTTGGAAAAGATGAGAGCGTGGAAGTGCCGAGCGTAGGTGGTCGTCCGCCACGGAGTCTGCAACGTCAGACACTGGCAGAGGTGATCGAGCC > minE/89480‑89617 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |