BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	974,093	A→G	7.4%	T290A (ACG→GCG)	lsrB →	AI2 transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	974,093	0	A	G	7.4%	311.8 / 13.1	108	T290A (ACG→GCG)	lsrB	AI2 transporter
Reads supporting (aligned to +/- strand): ref base A (57/43); new base G (4/4); total (61/47)
Fisher's exact test for biased strand distribution p-value = 7.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 125 total aligned reads displayed.

GATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTAT  >  minE/974024‑974161

gATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAAc                                                                     <  1:3103523/71‑1 (MQ=255)

gATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAAc                                                                     <  1:2341109/71‑1 (MQ=255)

 aTGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACg                                                                    <  1:447723/71‑1 (MQ=255)

 aTGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACg                                                                    <  1:65365/71‑1 (MQ=255)

     ggTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGc                                                                 >  1:3490478/1‑70 (MQ=255)

       ttCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGaaaa                                                                      <  1:224897/63‑1 (MQ=255)

       ttCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGaaaa                                                                      <  1:2709036/63‑1 (MQ=255)

        tCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACgg                                                                   >  1:1248294/1‑65 (MQ=255)

        tCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGAc                                                              >  1:2875170/1‑70 (MQ=255)

         cAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGAc                                                              >  1:735569/1‑69 (MQ=255)

         cAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGAc                                                              >  1:878364/1‑69 (MQ=255)

         cAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGAc                                                              >  1:1719783/1‑69 (MQ=255)

          aGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAg                                                           >  1:1346499/1‑71 (MQ=255)

          aGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAg                                                           <  1:2258897/71‑1 (MQ=255)

           gCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGc                                                          >  1:21158/1‑71 (MQ=255)

            cAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAGCGGGCGACAAGCt                                                         >  1:2220357/1‑71 (MQ=255)

             aaGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTg                                                        >  1:2203050/1‑71 (MQ=255)

                gCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACa                                                             >  1:1737642/1‑63 (MQ=255)

                gCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACa                                                             >  1:3244818/1‑63 (MQ=255)

                gCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACa                                                             >  1:351161/1‑63 (MQ=255)

                   aaaTTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTg                                                        <  1:2283001/65‑1 (MQ=255)

                   aaaTTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTg                                                        <  1:216928/65‑1 (MQ=255)

                   aaaTTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGa                                                      >  1:2940003/1‑67 (MQ=255)

                   aaaTTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGa                                                      >  1:1777695/1‑67 (MQ=255)

                   aaaTTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGa                                                      >  1:329174/1‑67 (MQ=255)

                   aaaTTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATc                                                  <  1:2043733/71‑1 (MQ=255)

                   aaaTTTCAGTGTATGTCGCGGATGAATTATTGAAAAAAGGATCAATGAAAACGGGCGACaa                                                            <  1:3561211/61‑1 (MQ=255)

                    aaTTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCa                                                 <  1:3078659/71‑1 (MQ=255)

                    aaTTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCa                                                 <  1:2173013/71‑1 (MQ=255)

                     aTTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAGCGGGCGACAAGCTGGATATc                                                  >  1:3298504/1‑69 (MQ=255)

                     aTTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGc                                                          >  1:1954382/1‑61 (MQ=255)

                          aGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCg                                           <  1:2579355/71‑1 (MQ=255)

                           gtgtATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGt                                          <  1:3455738/71‑1 (MQ=255)

                              tATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCg                                                                >  1:1753944/1‑46 (MQ=255)

                              tATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGc                                                          <  1:1727299/52‑1 (MQ=255)

                              tATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGa                                                      <  1:2772479/56‑1 (MQ=255)

                              tATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGa                                                      <  1:2312373/56‑1 (MQ=255)

                              tATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGa                                                      <  1:343530/56‑1 (MQ=255)

                                tGTCGCGGATGCATTATTGAAAAGAGGATCAATGaaaa                                                                      >  1:504268/1‑38 (MQ=255)

                                tGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACa                                                             >  1:2664420/1‑47 (MQ=255)

                                tGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGata                                                    >  1:299562/1‑56 (MQ=255)

                                tGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGt                                      >  1:1450892/1‑70 (MQ=255)

                                tGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGt                                      >  1:2658139/1‑70 (MQ=255)

                                tGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGt                                      >  1:1201226/1‑70 (MQ=255)

                                tGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTc                                     <  1:614321/71‑1 (MQ=255)

                                  tCGCGGATGCATTATTGAAAAAAGGATCAATGaaaa                                                                      >  1:3685485/1‑36 (MQ=255)

                                  tCGCGGATGCATTATTGAAAAAAGGATCAATGaaaa                                                                      >  1:2014395/1‑36 (MQ=255)

                                  tCGCGGATGCATTATTGAAAAAAGGATCAATGAAAGCGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAg                                   >  1:3637151/1‑71 (MQ=255)

                                      ggATGCATTATTGAAAAAAGGATCAATGAAAGCGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTg                               <  1:838986/71‑1 (MQ=255)

                                      ggATGCATTATTGAAAAAAGGATCAATGAAAGCGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTg                               <  1:3663530/71‑1 (MQ=255)

                                       gATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTg                               <  1:1184590/70‑1 (MQ=255)

                                        aTGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTg                               >  1:679245/1‑69 (MQ=255)

                                            attattGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGtct                         >  1:108540/1‑71 (MQ=255)

                                             ttattGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGtctc                        <  1:2878860/71‑1 (MQ=255)

                                             ttattGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGtctc                        <  1:2858134/71‑1 (MQ=255)

                                             ttattGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGtctc                        <  1:401888/71‑1 (MQ=255)

                                             ttattGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGtctc                        <  1:716338/71‑1 (MQ=255)

                                               attGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGata                                                    >  1:1103064/1‑41 (MQ=255)

                                               attGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGa                                                      >  1:2986136/1‑39 (MQ=255)

                                               attGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGa                                                      >  1:199303/1‑39 (MQ=255)

                                               attGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGa                                                      >  1:3121013/1‑39 (MQ=255)

                                               attGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGa                                                      >  1:88163/1‑39 (MQ=255)

                                               attGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGaa                             >  1:3034547/1‑64 (MQ=255)

                                                   aaaaaaGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCaa                   <  1:101477/70‑1 (MQ=255)

                                                   aaaaaaGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCaa                   <  1:1309448/70‑1 (MQ=255)

                                                   aaaaaaGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCaa                   <  1:333829/70‑1 (MQ=255)

                                                     aaaaGGATCAATGAAAACGGGCGACAAGCTGGatat                                                   <  1:3493623/36‑1 (MQ=255)

                                                     aaaaGGATCAATGAAAACGGGCGACAAGCTGGatat                                                   <  1:2855547/36‑1 (MQ=255)

                                                     aaaaGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCaaa                  >  1:1485882/1‑69 (MQ=255)

                                                     aaaaGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACa                <  1:2947946/71‑1 (MQ=255)

                                                      aaaGGATCAATGAAAACGGGCGACAAGCTGGatat                                                   <  1:13007/35‑1 (MQ=255)

                                                       aaGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGtctc                        >  1:3380779/1‑61 (MQ=255)

                                                       aaGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGtctc                        >  1:1089269/1‑61 (MQ=255)

                                                       aaGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGtctc                        >  1:2501551/1‑61 (MQ=255)

                                                       aaGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGtctc                        >  1:3463480/1‑61 (MQ=255)

                                                       aaGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGtct                         >  1:888992/1‑60 (MQ=255)

                                                        aGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCg             <  1:382321/71‑1 (MQ=255)

                                                         ggATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGtctc                        >  1:2315503/1‑59 (MQ=255)

                                                         ggATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGtctc                        >  1:2289781/1‑59 (MQ=255)

                                                         ggATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGtctc                        >  1:3447519/1‑59 (MQ=255)

                                                           aTCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTg                               <  1:2768539/50‑1 (MQ=255)

                                                           aTCAATGAAAACGGGCGACAAGATGGATATCAAGGGCGTAGGTCAGGTTg                               <  1:3356862/50‑1 (MQ=255)

                                                            tCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTg                               <  1:218103/49‑1 (MQ=255)

                                                             cAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAg        >  1:3459315/1‑71 (MQ=255)

                                                               aTGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAggg      >  1:1494640/1‑71 (MQ=255)

                                                               aTGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAg        <  1:2396623/69‑1 (MQ=255)

                                                                tGAAAGCGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAg                                   <  1:2758879/41‑1 (MQ=255)

                                                                tGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGt                           <  1:164609/49‑1 (MQ=255)

                                                                tGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGGGc     >  1:1768908/1‑71 (MQ=255)

                                                                tGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGGGc     >  1:3415695/1‑71 (MQ=255)

                                                                tGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGGGc     >  1:3132633/1‑71 (MQ=255)

                                                                 gAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGt                           <  1:1862512/48‑1 (MQ=255)

                                                                 gAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGGGc     >  1:1508650/1‑70 (MQ=255)

                                                                  aaagCGGGCGACAAGCTGGATATCAAGGGCGTAgg                                       <  1:3380147/35‑1 (MQ=255)

                                                                  aaaaCGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTa   >  1:1197084/1‑71 (MQ=255)

                                                                   aaaCGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTAt  <  1:423328/71‑1 (MQ=255)

                                                                   aaaCGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTAt  <  1:3269082/71‑1 (MQ=255)

                                                                    aaCGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTct         >  1:560867/1‑62 (MQ=255)

                                                                    aaCGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTAt  >  1:526928/1‑70 (MQ=255)

                                                                     aCGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGtt                                >  1:1691575/1‑39 (MQ=255)

GATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTAT  >  minE/974024‑974161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`