| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 988,137 | C→T | 10.0% | A793V (GCG→GTG) | ynfE → | oxidoreductase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 988,137 | 0 | C | T | 10.0% | 216.0 / 10.7 | 70 | A793V (GCG→GTG) | ynfE | oxidoreductase subunit |
| Reads supporting (aligned to +/- strand): ref base C (38/25); new base T (4/3); total (42/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCT > minE/988068‑988207 | tGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGc < 1:2258357/70‑1 (MQ=255)tGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCg < 1:3391165/71‑1 (MQ=255) tCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCg > 1:1325714/1‑69 (MQ=255) tCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGa < 1:858155/70‑1 (MQ=255) cGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaa < 1:2699108/71‑1 (MQ=255) cGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaa < 1:2818790/71‑1 (MQ=255) aaaTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCg < 1:1239386/71‑1 (MQ=255) aaaTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCg > 1:1221467/1‑71 (MQ=255) aaaTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCg > 1:2635735/1‑71 (MQ=255) aaTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCg < 1:151833/70‑1 (MQ=255) gcggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTaa > 1:402700/1‑70 (MQ=255) gcggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAAt > 1:1474647/1‑71 (MQ=255) gcggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAAt > 1:1501389/1‑71 (MQ=255) gcggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAAt > 1:1840596/1‑71 (MQ=255) gcggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAAt > 1:183870/1‑71 (MQ=255) cggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAAt < 1:407268/70‑1 (MQ=255) cTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTgg > 1:2683691/1‑71 (MQ=255) tgtgtgAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTa < 1:1443779/64‑1 (MQ=255) tgtgAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAAt > 1:1096262/1‑64 (MQ=255) tgtgAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAAt > 1:1398391/1‑64 (MQ=255) tgtgAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTc < 1:187340/70‑1 (MQ=255) gAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaa < 1:1682497/44‑1 (MQ=255) gAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaa < 1:3130649/44‑1 (MQ=255) gAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaa < 1:2379727/44‑1 (MQ=255) gCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaa > 1:752801/1‑70 (MQ=255) cTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaa > 1:34722/1‑70 (MQ=255) cTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaa > 1:2829146/1‑70 (MQ=255) cTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaa > 1:2632320/1‑70 (MQ=255) aCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCgaga > 1:1753739/1‑61 (MQ=255) aCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCgaga > 1:1679553/1‑61 (MQ=255) aCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCgag > 1:2270126/1‑60 (MQ=255) aCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGa < 1:984386/65‑1 (MQ=255) aCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGa < 1:2491769/65‑1 (MQ=255) aCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAat < 1:1409491/71‑1 (MQ=255) cACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAatat < 1:3014067/71‑1 (MQ=255) cACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAatat < 1:3082596/71‑1 (MQ=255) cACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAata < 1:938746/70‑1 (MQ=255) aCTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAatat > 1:3415244/1‑70 (MQ=255) ctGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTc > 1:1805583/1‑51 (MQ=255) ctGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTc > 1:601571/1‑51 (MQ=255) ctGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTc > 1:3194947/1‑51 (MQ=255) ctGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTc > 1:3132404/1‑51 (MQ=255) ctGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAAcc > 1:2093287/1‑71 (MQ=255) cgcCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAAcc > 1:1174709/1‑68 (MQ=255) cgcCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCAc < 1:3730349/71‑1 (MQ=255) tcccTTCGCCAGTGGTGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaa < 1:747772/56‑1 (MQ=25) gcCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAat > 1:2631930/1‑61 (MQ=255) gcCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAat > 1:729636/1‑61 (MQ=255) gcCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAat > 1:2092111/1‑61 (MQ=255) cccTTCGCCAGTGGTGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaa < 1:1215909/56‑1 (MQ=37) cccTTCGCCAGTGGTGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaa < 1:1291168/56‑1 (MQ=37) ccTTCGCCGGTGGTGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaaaa > 1:1040233/1‑57 (MQ=21) ccTTCGCCAGTGGTGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaaaa > 1:2372890/1‑57 (MQ=37) ccTTCGCCAGTGGTGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaaaa > 1:2219659/1‑57 (MQ=37) ccTTCGCCAGTGATGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaaaa > 1:1638431/1‑57 (MQ=25) ccTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGa > 1:3396006/1‑70 (MQ=255) cTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGAc > 1:3181267/1‑70 (MQ=255) ttCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaaaa < 1:95858/55‑1 (MQ=255) ttCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaaaa < 1:146156/55‑1 (MQ=255) ttCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaa < 1:3175784/52‑1 (MQ=255) ttCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACaa > 1:3740456/1‑71 (MQ=255) caccacTGGCGAAGGGAAACCCGCAGCACACTAAt > 1:613182/1‑35 (MQ=255) caccacTGGCGAAGGGAAACCCGCAGCACACTAAt > 1:2989082/1‑35 (MQ=255) caccacTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAAc > 1:3820309/1‑70 (MQ=255) accacTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCa > 1:2750904/1‑71 (MQ=255) cacTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaaa < 1:3236255/49‑1 (MQ=255) cacTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATa < 1:611858/71‑1 (MQ=255) acTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATa > 1:202251/1‑70 (MQ=255) tGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaa > 1:1183518/1‑44 (MQ=255) ggCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGa > 1:2764621/1‑59 (MQ=255) gCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAAt < 1:2959059/69‑1 (MQ=255) cGAATGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCt > 1:1567883/1‑71 (MQ=255) | TGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCT > minE/988068‑988207 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |