| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,008,960 | G→A | 11.3% | L98L (CTG→CTA) | folM → | dihydrofolate reductase isozyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,008,960 | 0 | G | A | 11.3% | 204.9 / 14.7 | 71 | L98L (CTG→CTA) | folM | dihydrofolate reductase isozyme |
| Reads supporting (aligned to +/- strand): ref base G (36/26); new base A (4/4); total (40/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01 | |||||||||||
CATGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAACCATGCGCTGGA > minE/1008895‑1009028 | cATGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGa > 1:2022046/1‑71 (MQ=255)cATGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGa > 1:2505838/1‑71 (MQ=255)cATGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGa > 1:1538924/1‑71 (MQ=255) aTGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTAGCCGAc > 1:928713/1‑71 (MQ=255) aTGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTAGCCGAc > 1:1733008/1‑71 (MQ=255) tGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg < 1:3442281/71‑1 (MQ=255) cGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTAGCCGACGTACTGGc > 1:3096909/1‑71 (MQ=255) cGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTAGCCGACGTACTGGc > 1:2098179/1‑71 (MQ=255) cTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTg < 1:3140984/70‑1 (MQ=255) cTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTg < 1:2749661/70‑1 (MQ=255) ttttGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCat < 1:3735903/70‑1 (MQ=255) ttttGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCat > 1:2752781/1‑70 (MQ=255) ttttGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCat > 1:1701222/1‑70 (MQ=255) ttttGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCat < 1:1041558/70‑1 (MQ=255) ttttGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCat < 1:629509/70‑1 (MQ=255) ttttGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCa < 1:2174603/69‑1 (MQ=255) tttGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTa > 1:892153/1‑58 (MQ=255) ttGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGa > 1:3296841/1‑53 (MQ=255) ttGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGa > 1:2676694/1‑53 (MQ=255) ttGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatgat > 1:792688/1‑71 (MQ=255) ttGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatga < 1:1717110/70‑1 (MQ=255) gCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatgatg > 1:2105339/1‑70 (MQ=255) cATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatgatg > 1:653997/1‑69 (MQ=255) cATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGc < 1:3246801/70‑1 (MQ=255) aaCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatgatg > 1:3115346/1‑66 (MQ=255) aaCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatgatg > 1:10702/1‑66 (MQ=255) aaCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatgatg > 1:577385/1‑66 (MQ=255) aaCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatgatg > 1:3478766/1‑66 (MQ=255) aaCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatgatg > 1:2365689/1‑66 (MQ=255) aaCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatgatg > 1:1963250/1‑66 (MQ=255) aaCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatgatg > 1:1875320/1‑66 (MQ=255) aCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATc > 1:3701897/1‑71 (MQ=255) gTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGtt < 1:2319177/71‑1 (MQ=255) tGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGtt > 1:1630902/1‑70 (MQ=255) cGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTaa < 1:3233342/70‑1 (MQ=255) gTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGtt < 1:2473100/67‑1 (MQ=255) tGGATGGCGGAAAAACCGGGTGCGCCACTAGCCGACGTACTGGCTTGCATGATGCAGa < 1:760720/58‑1 (MQ=255) gATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATAccc > 1:2256862/1‑71 (MQ=255) gATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATAccc > 1:3355078/1‑71 (MQ=255) gATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATAccc > 1:1974251/1‑71 (MQ=255) gATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATACACGTTAATAccc > 1:212901/1‑71 (MQ=255) gCGGAAAAACCGGGTGCGCCACTTGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCAt < 1:3260704/70‑1 (MQ=255) gCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCAt < 1:3055029/70‑1 (MQ=255) gCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCAt < 1:1757784/70‑1 (MQ=255) gCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCAt < 1:460858/70‑1 (MQ=255) gCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCAt < 1:2922072/70‑1 (MQ=255) cGGAAAAACCGGGTGCGCCACTAGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATAc < 1:881431/71‑1 (MQ=255) cGGAAAAACCGGGTGCGCCACTAGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATAc < 1:1508201/71‑1 (MQ=255) cGGAAAAACCGGGTGCGCCACTAGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATAc < 1:2685411/71‑1 (MQ=255) ggAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATAcc > 1:3502031/1‑71 (MQ=255) ggAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATAcc > 1:2369780/1‑71 (MQ=255) gAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGc < 1:2535201/46‑1 (MQ=255) gAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATc < 1:1911871/51‑1 (MQ=255) gAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATc < 1:1294290/51‑1 (MQ=255) aaaaCCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCa > 1:1110766/1‑51 (MQ=255) aaaaCCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGc > 1:2066756/1‑71 (MQ=255) aaaCCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATAcc > 1:1515652/1‑60 (MQ=255) aaaCCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATAcc > 1:2923605/1‑60 (MQ=255) aaaCCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCt > 1:2614677/1‑71 (MQ=255) aaCCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTc < 1:1628381/71‑1 (MQ=255) aaCCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTc < 1:1680472/71‑1 (MQ=255) aCCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTc > 1:320394/1‑70 (MQ=255) aCCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCa < 1:1617249/71‑1 (MQ=255) ggTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTaa < 1:88400/50‑1 (MQ=255) ggTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAACCa > 1:2528083/1‑71 (MQ=255) ggTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAACCa > 1:2913333/1‑71 (MQ=255) ggTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAACCa > 1:2222451/1‑71 (MQ=255) ggTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAACCa < 1:888050/71‑1 (MQ=255) gTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAACCAt > 1:1108117/1‑71 (MQ=255) cTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAACCATGCGCTGGa < 1:377234/71‑1 (MQ=255) cTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAACCATGCGCTGGa < 1:3414497/71‑1 (MQ=255) ggCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTc < 1:2796979/55‑1 (MQ=255) ggCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAACCATGCGCTGGa > 1:770773/1‑69 (MQ=255) | CATGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAACCATGCGCTGGA > minE/1008895‑1009028 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |