| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,269,969 | G→A | 5.5% | A74T (GCA→ACA) | yedP → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,269,969 | 0 | G | A | 5.5% | 242.5 / 3.4 | 72 | A74T (GCA→ACA) | yedP | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (44/24); new base A (2/2); total (46/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 | |||||||||||
ATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCC > minE/1269899‑1270038 | aTCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGgcg < 1:3578257/71‑1 (MQ=255)aTCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGgcg < 1:683164/71‑1 (MQ=255) ggAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTg > 1:2734132/1‑70 (MQ=255) ggAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGa > 1:1219193/1‑71 (MQ=255) ggAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGa < 1:68525/71‑1 (MQ=255) ggAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGa < 1:2292135/71‑1 (MQ=255) tGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATcc > 1:630288/1‑69 (MQ=255) gCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGc > 1:871460/1‑71 (MQ=255) gCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGc > 1:3074721/1‑71 (MQ=255) cTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCt > 1:7456/1‑71 (MQ=255) tACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCtt < 1:3860380/69‑1 (MQ=255) cTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCt > 1:3280054/1‑70 (MQ=255) cTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCt > 1:1366953/1‑70 (MQ=255) cTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTg < 1:1454780/71‑1 (MQ=255) gCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAg > 1:3579579/1‑70 (MQ=255) gCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAg > 1:266537/1‑70 (MQ=255) gCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAg > 1:1457133/1‑70 (MQ=255) gCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAg > 1:1980878/1‑70 (MQ=255) cAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCa > 1:3618209/1‑51 (MQ=255) cAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCa > 1:2484377/1‑51 (MQ=255) cAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCa > 1:2372094/1‑51 (MQ=255) aaaaaCGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGa < 1:2167531/53‑1 (MQ=255) aaaaCGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATg > 1:539934/1‑71 (MQ=255) cGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAg > 1:222229/1‑71 (MQ=255) cGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAg < 1:1584245/71‑1 (MQ=255) gTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAgg < 1:2731803/71‑1 (MQ=255) gggCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGgaga > 1:3673649/1‑70 (MQ=255) cTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGa > 1:3301009/1‑71 (MQ=255) tACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCtt < 1:2373376/47‑1 (MQ=255) tACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCtt < 1:2703312/47‑1 (MQ=255) tACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCtt < 1:1486573/47‑1 (MQ=255) aCAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCATGAGATAGACg > 1:85694/1‑71 (MQ=255) aCAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATa < 1:637218/67‑1 (MQ=255) aCAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATa < 1:1197097/67‑1 (MQ=255) cAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACgg < 1:2292731/71‑1 (MQ=255) cAAGGTTTACCGCTGATTGCCGAAAATGGCACAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACgg > 1:1323304/1‑71 (MQ=255) cAAGGTTTACCGCTGATTGCCGAAAATGGCACAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACgg > 1:689360/1‑71 (MQ=255) aaGGTTTACCGCTGATTGCCGATAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGa > 1:2495758/1‑67 (MQ=255) aaGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGa > 1:2626635/1‑67 (MQ=255) aaGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGa > 1:2346876/1‑67 (MQ=255) aaGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGt > 1:3505969/1‑71 (MQ=255) aaGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGt > 1:2235761/1‑71 (MQ=255) aaGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGt > 1:2722383/1‑71 (MQ=255) ggTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGttt > 1:288714/1‑71 (MQ=255) ggTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGttt < 1:2627160/71‑1 (MQ=255) gTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGc < 1:1347766/48‑1 (MQ=255) gTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGc < 1:1253327/48‑1 (MQ=255) gTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGc < 1:2362431/48‑1 (MQ=255) gTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGtttt > 1:857117/1‑71 (MQ=255) tttATCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTc < 1:721262/71‑1 (MQ=255) tttACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTc < 1:2605183/71‑1 (MQ=255) ttACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTc > 1:875849/1‑70 (MQ=255) tACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCa > 1:305076/1‑71 (MQ=255) cGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCAc > 1:2304905/1‑69 (MQ=255) cTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGcat > 1:2208738/1‑71 (MQ=255) cTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGcat > 1:482351/1‑71 (MQ=255) cTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGcat > 1:2725640/1‑71 (MQ=255) cTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGcat > 1:2314136/1‑71 (MQ=255) tGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGc > 1:1074100/1‑45 (MQ=255) gATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGcatca > 1:3632225/1‑71 (MQ=255) aTTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGcat < 1:2268728/68‑1 (MQ=255) ttGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCatcatc < 1:643888/71‑1 (MQ=255) tGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACgg > 1:325937/1‑54 (MQ=255) tGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACgg > 1:1070822/1‑54 (MQ=255) gCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGTCAGGAGATAGACGGTTTTCCACGCATCAtct < 1:501713/70‑1 (MQ=255) gCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCAtct < 1:56516/70‑1 (MQ=255) gCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCAtct > 1:2834133/1‑70 (MQ=255) gAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAgg > 1:2460879/1‑71 (MQ=255) gAAAATGGCACAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAgg < 1:827369/71‑1 (MQ=255) gAAAATGGCACAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAgg < 1:3602825/71‑1 (MQ=255) aaaTGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGcat > 1:2443269/1‑60 (MQ=255) aaaTGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTa > 1:2534917/1‑71 (MQ=255) aaaTGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTa > 1:3789481/1‑71 (MQ=255) aaTGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTAt < 1:690861/71‑1 (MQ=255) gcgcAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGc < 1:1970771/71‑1 (MQ=255) cgcAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACTGTTTTCCACGCTTCATCTCAGGTATTAGcc < 1:640738/71‑1 (MQ=255) cgcAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGcc < 1:2385726/71‑1 (MQ=255) | ATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATGGCGCAGTGATCCAGCTTGCTGAGCAATGGCAGGAGATAGACGGTTTTCCACGCATCATCTCAGGTATTAGCC > minE/1269899‑1270038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |