Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 204,511 C→T 100% intergenic (+111/‑73) alaV → / → rrlH tRNA‑Ala/23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE204,5110CT100.0% 20.1 / NA 10intergenic (+111/‑73)alaV/rrlHtRNA‑Ala/23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/4);  total (6/4)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AAAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGG  >  minE/204491‑204577
                    |                                                                  
aaaaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                  <  1:3525645/71‑1 (MQ=9)
 aaaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                  >  1:1513005/1‑70 (MQ=255)
 aaaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                  >  1:2377978/1‑70 (MQ=255)
 aaaTTGAAACACTGAACAATGAAAGTTGTTAGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                  >  1:3399747/1‑70 (MQ=11)
  aaTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGc                  >  1:2559007/1‑69 (MQ=255)
   aTTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAg               <  1:830214/71‑1 (MQ=255)
     tGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACAATGATGGATCGCAAGa              >  1:1154188/1‑70 (MQ=11)
     tGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACAATGATGGATCGCAAGa              >  1:474914/1‑70 (MQ=11)
       aaaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc           <  1:1573698/71‑1 (MQ=255)
       aaaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc           <  1:2388208/71‑1 (MQ=255)
                acaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCggg  >  1:2661531/1‑71 (MQ=255)
                    |                                                                  
AAAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGG  >  minE/204491‑204577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑