| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,440,764 | C→G | 8.8% | intergenic (‑463/‑276) | ompC ← / → rcsD | outer membrane porin protein C/phosphotransfer intermediate protein in two‑component regulatory system with RcsBC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,440,764 | 0 | C | G | 8.8% | 176.2 / 6.2 | 57 | intergenic (‑463/‑276) | ompC/rcsD | outer membrane porin protein C/phosphotransfer intermediate protein in two‑component regulatory system with RcsBC |
| Reads supporting (aligned to +/- strand): ref base C (30/22); new base G (3/2); total (33/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
TTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAA > minE/1440696‑1440830 | tttCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTAc < 1:96187/69‑1 (MQ=255)tttCAACCGGATGCCTCGCATTCGG‑TTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACca > 1:266635/1‑70 (MQ=255) tCAACCGGATGCCTAGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACca > 1:454012/1‑69 (MQ=255) aCCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGa > 1:856380/1‑70 (MQ=255) aCCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGa > 1:434247/1‑70 (MQ=255) ccGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGaaa < 1:337713/71‑1 (MQ=255) ccGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGaaa < 1:637002/71‑1 (MQ=255) gCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGaaaaa > 1:3144014/1‑67 (MQ=255) tCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGaaa < 1:2264403/62‑1 (MQ=255) cGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACg > 1:3134349/1‑71 (MQ=255) gCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACcac > 1:3460926/1‑55 (MQ=255) gCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACtata > 1:1901099/1‑67 (MQ=255) gCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACtata > 1:3781931/1‑67 (MQ=255) ttCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGaaaa < 1:1630708/58‑1 (MQ=255) ttCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTg < 1:2373784/71‑1 (MQ=255) tCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGt > 1:2749523/1‑71 (MQ=255) tCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGt > 1:2736434/1‑71 (MQ=255) cGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAAc > 1:545561/1‑58 (MQ=255) cGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGt > 1:302305/1‑70 (MQ=255) cGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGt < 1:1638843/70‑1 (MQ=255) cGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGt > 1:1365065/1‑70 (MQ=255) cGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGt > 1:2356259/1‑70 (MQ=255) ggTTTTTTTTACCCTTCTGTACACACTTTTCATTATTCTGTGCTACCACAGAAAAAc > 1:2962809/1‑57 (MQ=255) gTTTTTTTTACCCTTCTTTACACACTTTTCATTTTTCTGTGGTAGcaca < 1:3231489/49‑1 (MQ=37) gTTTTTTTTACCCTTCTTTACACACTTTTCATTTTTCTGTGGTAGcaca < 1:206770/49‑1 (MQ=37) gTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGtt < 1:308099/69‑1 (MQ=255) gTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTaa > 1:1804927/1‑71 (MQ=255) ttttttACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTa > 1:3769413/1‑71 (MQ=255) ccTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGaaaaa < 1:2134269/44‑1 (MQ=255) tttACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAaataat > 1:2786229/1‑71 (MQ=255) acacacTTTTCATTATTCTGTGCTACCACAGAAAAAc < 1:2904622/37‑1 (MQ=255) acacacTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAATTATTTGTGAAATAGTTa < 1:917240/70‑1 (MQ=37) cacacTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAAc < 1:1093767/71‑1 (MQ=255) cacacTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAAc < 1:1154899/71‑1 (MQ=255) ttttCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGtt < 1:3229648/44‑1 (MQ=255) ttttCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGtt < 1:635696/44‑1 (MQ=255) ttttCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGtt < 1:2551208/44‑1 (MQ=255) ttttCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGtt < 1:3767507/44‑1 (MQ=255) ttttCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAaat < 1:3234379/59‑1 (MQ=255) ttttCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAAtt < 1:2204524/63‑1 (MQ=255) tttCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCg > 1:1041159/1‑70 (MQ=255) ttCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTa > 1:3175263/1‑62 (MQ=255) ttCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTa > 1:2061771/1‑62 (MQ=255) ttCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGc < 1:1649211/70‑1 (MQ=255) ttCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCa > 1:978617/1‑71 (MQ=255) tCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCa > 1:2335574/1‑70 (MQ=255) tCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCa > 1:1328584/1‑70 (MQ=255) ttCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTc < 1:3429188/71‑1 (MQ=255) cTGTGGTAGCACAGAATAACTATAACGCTTGTTAAc > 1:553555/1‑36 (MQ=18) cTGTGGTAGCACAGAATAACTATAACGCTTGTTAAc > 1:934431/1‑36 (MQ=18) cTGTGGTAGCACAGAATAACTATAACGCTTGTTAAc > 1:958303/1‑36 (MQ=18) cTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTcc < 1:2727467/70‑1 (MQ=255) cTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTcc < 1:300053/70‑1 (MQ=255) tgtgCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAg > 1:2709049/1‑71 (MQ=255) cTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCaa > 1:2853759/1‑70 (MQ=255) tACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCg > 1:1582627/1‑54 (MQ=255) tACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCg > 1:2983574/1‑54 (MQ=255) aCCACAGAAAAACTATAACGCTTGTTAACTATTTCACAaataat > 1:1451455/1‑44 (MQ=255) | TTTCAACCGGATGCCTCGCATTCGGTTTTTTTTACCCTTCTTTACACACTTTTCATTATTCTGTGCTACCACAGAAAAACTATAACGCTTGTTAACTATTTCACAAATAATTAACATCCGCATAATTTCCAGCAA > minE/1440696‑1440830 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |