| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,477,381 | A→G | 9.2% | E48G (GAA→GGA) | yfaH → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,477,381 | 0 | A | G | 9.2% | 210.3 / 12.1 | 76 | E48G (GAA→GGA) | yfaH | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (37/32); new base G (4/3); total (41/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01 | |||||||||||
TTGTGTTCCGTTCCTCTCGAACCAACTTTCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAATTAGTGGCGATTGGAAAAATTTGATATGA > minE/1477311‑1477450 | ttGTGTTCCGTTCCTCTCGAACCAACTTTCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGa < 1:3433701/71‑1 (MQ=255) gtgtTCCGTTCCTCTCGAACCAACTTTCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGaa < 1:1381983/70‑1 (MQ=255) ttccgttccTCTCGAACCAACTTTCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAgg > 1:3679546/1‑69 (MQ=255) tccgttccTCTCGAACCAACTTTCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAg > 1:389414/1‑71 (MQ=255) tccgttccTCTCGAACCAACTTTCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAg > 1:337949/1‑71 (MQ=255) cgttccTCTCGAACCAACTTTCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTc > 1:729827/1‑71 (MQ=255) cgttccTCTCGAACCAACTTTCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTc > 1:790560/1‑71 (MQ=255) cgttccTCTCGAACCAACTTTCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTc > 1:2566417/1‑71 (MQ=255) gttccTCTCGAACCAACTTTCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGGAGGCAGt > 1:1973578/1‑69 (MQ=255) tccTCTCGAACCAACTTTCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCAc > 1:1825708/1‑71 (MQ=255) tccTCTCGAACCAACTTTCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCAc > 1:3257519/1‑71 (MQ=255) ctctCGAACCAACTTTCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTg > 1:968041/1‑71 (MQ=255) ctctCGAACCAACTTTCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTg > 1:2055919/1‑71 (MQ=255) tcGAACCAACTTTCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGt < 1:1391161/69‑1 (MQ=255) cGAACCAACTTTCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGtttt > 1:3023670/1‑71 (MQ=255) gAACCAACTTTCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGtttt > 1:2222871/1‑70 (MQ=255) gAACCAACTTTCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGtt < 1:1697952/68‑1 (MQ=255) gaaCTTTCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGt < 1:2469717/62‑1 (MQ=255) cAACTTTCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGtttt < 1:482106/66‑1 (MQ=255) aaCTTTCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGaa < 1:1308659/49‑1 (MQ=255) aaCTTTCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTtactac < 1:1426976/71‑1 (MQ=255) aCTTTCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACa < 1:619827/71‑1 (MQ=255) aCTTTCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACa < 1:3321110/71‑1 (MQ=255) cTTTCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGtt < 1:2505479/61‑1 (MQ=255) tttCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACaaa > 1:2553558/1‑71 (MQ=255) tttCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACaaa > 1:1829472/1‑71 (MQ=255) tttCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACaaa > 1:3840183/1‑71 (MQ=255) tCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGGAGGCAGTCCACTGTTTTTACTTCaaa < 1:68300/69‑1 (MQ=255) tCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAAtgtg < 1:71993/69‑1 (MQ=255) tCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAAtgtg < 1:67498/69‑1 (MQ=255) cGACATATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCAt > 1:569340/1‑71 (MQ=255) cGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCAt > 1:1327711/1‑71 (MQ=255) cGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCAt > 1:2816028/1‑71 (MQ=255) aCAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGtttt < 1:2078386/53‑1 (MQ=255) aCAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGtttt < 1:1306377/53‑1 (MQ=255) aCAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGtttt < 1:1178987/53‑1 (MQ=255) cAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGGAGGCAGTCCACTGTTTTTACTACAAATGTGCATgg > 1:3512810/1‑70 (MQ=255) aTTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATgg > 1:1696064/1‑67 (MQ=255) aTTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGa > 1:3751674/1‑68 (MQ=255) tttCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGtt < 1:1781267/46‑1 (MQ=255) ttCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGtttt > 1:2280433/1‑47 (MQ=255) tCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTc > 1:718322/1‑37 (MQ=255) tCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGtt > 1:3452550/1‑44 (MQ=255) tCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAAt < 1:1137569/70‑1 (MQ=255) tCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAAt > 1:990131/1‑70 (MQ=255) gTTGCTGGCTAAGGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAATTAg > 1:163391/1‑71 (MQ=255) ttGCTGGCTAATGAAAAGCCGGTAGAAGGCAGTCCACTGttttt < 1:2919204/44‑1 (MQ=255) ttGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAAc < 1:2590640/64‑1 (MQ=255) ttGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAAc < 1:2301706/64‑1 (MQ=255) tGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAAc < 1:238515/63‑1 (MQ=255) tGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAATTAGt < 1:1079315/67‑1 (MQ=255) tGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAATTAGt < 1:2199667/67‑1 (MQ=255) tGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAATTAGt < 1:2949005/67‑1 (MQ=255) gCTAAAGAAAAGCCGGTAGGAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAATTAGTGGCGAt < 1:3317802/71‑1 (MQ=255) gCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAATTAGTGGCGa < 1:1903526/70‑1 (MQ=255) gCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAATTAGTGGCGa < 1:1285470/70‑1 (MQ=255) gCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAATTAGTGGCGAt > 1:3512113/1‑71 (MQ=255) cTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAAtt < 1:2304052/61‑1 (MQ=255) cTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAAtt < 1:2580417/61‑1 (MQ=255) cTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAAtt < 1:2575846/61‑1 (MQ=255) tAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAATTAGTGGCGAtt > 1:1364438/1‑70 (MQ=255) tAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAATTAGTGGCGATTg > 1:3235628/1‑71 (MQ=255) tAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTATTTACTACAAATGTGCATGGAACAATTAGTGGCGATTg > 1:1097500/1‑71 (MQ=255) aaaGAAAAGCCGGTAGGAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAATTAGTGGCGATTg > 1:183966/1‑70 (MQ=255) aaaGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAATTAGTGGCGATTg > 1:2532432/1‑70 (MQ=255) aaGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAATTAGTGGCGATTgg > 1:3430131/1‑70 (MQ=255) aaGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAATTAGTGGCGATTgg > 1:2453641/1‑70 (MQ=255) aaGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAATTAGTGGCGATTgg > 1:738760/1‑70 (MQ=255) aaGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAATTAGTGGCGATTgg > 1:1237709/1‑70 (MQ=255) aaaaGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAATTAGTGGCGAt < 1:207406/64‑1 (MQ=255) aGCCGGTAGGAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAATTAGTGGCGATTGGaaaa < 1:2453825/68‑1 (MQ=255) gCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAATTAGTGGCGATTGGAAAAAttt < 1:3785558/71‑1 (MQ=255) gCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAATTAGTGGCGATTGGAAAAAttt < 1:2972804/71‑1 (MQ=255) gCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAATTAGTGGCGATTGGAAAAAtt > 1:1291707/1‑70 (MQ=255) ccGTTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAATTAGTGGCGATTGGAAAAAttt > 1:1542802/1‑70 (MQ=255) ccGGTAGGAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAATTAGTGGCGATTGGAAAAAt > 1:374875/1‑68 (MQ=255) cGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAATTAGTGGCGATTGGAAAAATTTGa < 1:1260147/71‑1 (MQ=255) aGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAATTAGTGGCGAtt > 1:317238/1‑55 (MQ=255) gAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAATTAGTGGCGATTGGAAAAATTTGATATga < 1:2107833/71‑1 (MQ=255) | TTGTGTTCCGTTCCTCTCGAACCAACTTTCTATCGACAGATTTCGTTGCTGGCTAAAGAAAAGCCGGTAGAAGGCAGTCCACTGTTTTTACTACAAATGTGCATGGAACAATTAGTGGCGATTGGAAAAATTTGATATGA > minE/1477311‑1477450 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |