| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,642,784 | T→A | 10.0% | P74P (CCA→CCT) | ypfJ ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,642,784 | 0 | T | A | 10.0% | 136.5 / 7.8 | 50 | P74P (CCA→CCT) | ypfJ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (24/21); new base A (3/2); total (27/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01 | |||||||||||
GTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAA > minE/1642720‑1642851 | gtCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCt < 1:89460/70‑1 (MQ=255) tCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTa < 1:2121330/70‑1 (MQ=255) aGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGAc > 1:881544/1‑71 (MQ=255) aGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGAc > 1:2226175/1‑71 (MQ=255) tgtCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACcgc < 1:943473/71‑1 (MQ=255) tgtCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACcgc < 1:859839/71‑1 (MQ=255) tgtCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACcgc < 1:252180/71‑1 (MQ=255) gtCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACcgc < 1:1612845/70‑1 (MQ=255) ttCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGtt > 1:444033/1‑70 (MQ=255) gTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGAt > 1:961851/1‑66 (MQ=255) gTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGAt > 1:1425229/1‑66 (MQ=255) gTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGAt > 1:3324329/1‑66 (MQ=255) ttGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGAttgttgt < 1:2376230/71‑1 (MQ=255) tGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGAcc > 1:3216675/1‑56 (MQ=255) tGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGa > 1:2827736/1‑63 (MQ=255) tGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGAttgttgt > 1:1070948/1‑70 (MQ=255) gCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTaa > 1:3745499/1‑49 (MQ=255) gCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGAttgttgttg > 1:2448164/1‑71 (MQ=255) gCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGAttgttgtt < 1:1704458/70‑1 (MQ=255) ccAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCt < 1:2254743/46‑1 (MQ=255) ccAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCt < 1:2119749/46‑1 (MQ=255) ccAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGAttgttgtt < 1:855793/69‑1 (MQ=255) cAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTAGGGCTAATTGACCGCGTTGATTGCTGTTgg < 1:3752530/70‑1 (MQ=255) cAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTAGGGCTAATTGACCGCGTTGATTGCTGTTgg < 1:824030/70‑1 (MQ=255) gAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTAGGGCTAATTGACCGCGTTGATTGCTGTTGGGa > 1:351067/1‑70 (MQ=255) gAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTAGGGCTAATTGACCGCGTTGATTGCTGTTGGGa > 1:3646630/1‑70 (MQ=255) gAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTAGGGCTAATTGACCGCGTTGATTGCTGTTGGGa > 1:2135943/1‑70 (MQ=255) aCCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACcgc < 1:1282692/48‑1 (MQ=255) aCCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCgg > 1:853570/1‑71 (MQ=255) ccGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGCCCGCGTTGAttgttgt > 1:2566813/1‑59 (MQ=255) ggTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGcc < 1:1482181/71‑1 (MQ=255) gTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGccc < 1:2079174/71‑1 (MQ=255) gAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCg > 1:224524/1‑70 (MQ=255) ttGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGtcat > 1:3421468/1‑70 (MQ=255) ttGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGtcat > 1:2473393/1‑70 (MQ=255) tGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGt > 1:847699/1‑66 (MQ=255) gCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGa < 1:1593337/35‑1 (MQ=255) ttCGTCTTCATTTGGGCTAATTGACCGCGTTGAtt > 1:573742/1‑35 (MQ=255) tCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCg < 1:1826230/69‑1 (MQ=255) tCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGt > 1:3695849/1‑71 (MQ=255) ttCATTTGGGCTAATTGACCGCGTTGAttgttgttg > 1:3652310/1‑36 (MQ=255) ttCATTTGGGCTAATTGACCGCGTTGAttgttgttg > 1:1251970/1‑36 (MQ=255) ttCATTTGGGCTAATTGACCGCGTTGAttgttgttg > 1:3326141/1‑36 (MQ=255) ttCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCg < 1:3158454/44‑1 (MQ=255) tCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAAt > 1:1432580/1‑70 (MQ=255) cATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGc > 1:779590/1‑44 (MQ=255) cATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATc < 1:2681094/70‑1 (MQ=255) cATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATc < 1:3492781/70‑1 (MQ=255) cATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATc < 1:1098881/70‑1 (MQ=255) aTTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCaa < 1:922170/71‑1 (MQ=255) tttGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTg < 1:2821461/44‑1 (MQ=255) | GTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAA > minE/1642720‑1642851 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |