| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,642,808 | T→C | 15.6% | Q66Q (CAA→CAG) | ypfJ ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,642,808 | 0 | T | C | 15.6% | 77.0 / 7.6 | 32 | Q66Q (CAA→CAG) | ypfJ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (15/12); new base C (3/2); total (18/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCA > minE/1642741‑1642875 | ttGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGAttgttgt < 1:2376230/71‑1 (MQ=255) tGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGAttgttgt > 1:1070948/1‑70 (MQ=255) gCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGAttgttgttg > 1:2448164/1‑71 (MQ=255) gCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGAttgttgtt < 1:1704458/70‑1 (MQ=255) ccAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGAttgttgtt < 1:855793/69‑1 (MQ=255) cAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTAGGGCTAATTGACCGCGTTGATTGCTGTTgg < 1:824030/70‑1 (MQ=255) cAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTAGGGCTAATTGACCGCGTTGATTGCTGTTgg < 1:3752530/70‑1 (MQ=255) gAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTAGGGCTAATTGACCGCGTTGATTGCTGTTGGGa > 1:3646630/1‑70 (MQ=255) gAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTAGGGCTAATTGACCGCGTTGATTGCTGTTGGGa > 1:2135943/1‑70 (MQ=255) gAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTAGGGCTAATTGACCGCGTTGATTGCTGTTGGGa > 1:351067/1‑70 (MQ=255) aCCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCgg > 1:853570/1‑71 (MQ=255) ccGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGCCCGCGTTGAttgttgt > 1:2566813/1‑59 (MQ=255) ggTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGcc < 1:1482181/71‑1 (MQ=255) gTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGccc < 1:2079174/71‑1 (MQ=255) gAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCg > 1:224524/1‑70 (MQ=255) ttGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGtcat > 1:3421468/1‑70 (MQ=255) ttGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGtcat > 1:2473393/1‑70 (MQ=255) tGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGt > 1:847699/1‑66 (MQ=255) tCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCg < 1:1826230/69‑1 (MQ=255) tCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGt > 1:3695849/1‑71 (MQ=255) ttCATTTGGGCTAATTGACCGCGTTGAttgttgttg > 1:3326141/1‑36 (MQ=255) ttCATTTGGGCTAATTGACCGCGTTGAttgttgttg > 1:3652310/1‑36 (MQ=255) ttCATTTGGGCTAATTGACCGCGTTGAttgttgttg > 1:1251970/1‑36 (MQ=255) ttCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCg < 1:3158454/44‑1 (MQ=255) tCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAAt > 1:1432580/1‑70 (MQ=255) cATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGc > 1:779590/1‑44 (MQ=255) cATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATc < 1:2681094/70‑1 (MQ=255) cATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATc < 1:3492781/70‑1 (MQ=255) cATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATc < 1:1098881/70‑1 (MQ=255) aTTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCaa < 1:922170/71‑1 (MQ=255) tttGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTg < 1:2821461/44‑1 (MQ=255) aaTTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCAtag > 1:3537444/1‑70 (MQ=255) aTTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCAtag > 1:1136578/1‑69 (MQ=255) ttGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCAtagtag > 1:3510671/1‑71 (MQ=255) gACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACAcc > 1:3164573/1‑62 (MQ=255) gACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGcc > 1:312683/1‑71 (MQ=255) aCCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCg < 1:2890293/47‑1 (MQ=255) ttGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAAcc < 1:3789351/71‑1 (MQ=255) ttGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAAcc < 1:2322778/71‑1 (MQ=255) gATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTa > 1:346820/1‑43 (MQ=255) gATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTa > 1:1594696/1‑43 (MQ=255) ttgttgttgGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCa > 1:3628796/1‑71 (MQ=255) ttgttgttgGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCa > 1:2532230/1‑71 (MQ=255) | TTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCA > minE/1642741‑1642875 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |