| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,682,955 | G→A | 8.6% | R673R (CGC→CGT) | yfhM ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,682,955 | 0 | G | A | 8.6% | 179.2 / 5.9 | 58 | R673R (CGC→CGT) | yfhM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (27/26); new base A (2/3); total (29/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
AGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCTTGCGCATCGCTATAAAC > minE/1682890‑1683023 | aGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGtt < 1:1387218/70‑1 (MQ=255)aGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTc < 1:2369328/71‑1 (MQ=255)aGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTc < 1:1734531/71‑1 (MQ=255) gATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGtt > 1:1648911/1‑69 (MQ=255) gATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTcg > 1:1327482/1‑71 (MQ=255) gATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTc > 1:3499686/1‑70 (MQ=255) aTCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTcgc > 1:1388647/1‑71 (MQ=255) aTCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTcgc > 1:2531200/1‑71 (MQ=255) cTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTcgc > 1:1593027/1‑69 (MQ=255) tttGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAAt > 1:3487150/1‑70 (MQ=255) ttGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATc < 1:3828676/70‑1 (MQ=255) aaCTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCAc < 1:2872646/71‑1 (MQ=255) cTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCa < 1:2292902/68‑1 (MQ=255) tgtgACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGaa > 1:1949799/1‑58 (MQ=255) tgtgACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATc > 1:323800/1‑60 (MQ=255) tgtgACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATc > 1:2297311/1‑60 (MQ=255) tgtgACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATc > 1:1770714/1‑60 (MQ=255) tgtgACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAgg < 1:1725243/63‑1 (MQ=255) tgACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTg < 1:1502088/69‑1 (MQ=255) tGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGgcgc < 1:2139421/60‑1 (MQ=255) tGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGgcgc < 1:3318919/60‑1 (MQ=255) tGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGgcgc < 1:282752/60‑1 (MQ=255) tGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGgcgc < 1:2166296/60‑1 (MQ=255) tGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGcc > 1:336674/1‑71 (MQ=255) gCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGccc > 1:2771720/1‑71 (MQ=255) ccAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGccc < 1:230868/70‑1 (MQ=255) ccAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGccc < 1:394350/70‑1 (MQ=255) cAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGACGTTCGCGAAt < 1:2724926/50‑1 (MQ=255) cAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGACGTTCGCGAAt < 1:15625/50‑1 (MQ=255) cAGCCTTCATCTTCTGACCAGATCCAGTAGTAATCGCGACGTTCGCGAAt < 1:674248/50‑1 (MQ=255) gCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGaa < 1:2841289/47‑1 (MQ=255) gCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAg < 1:3561060/51‑1 (MQ=255) gCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGACGTTCGCGAATCa > 1:2568145/1‑50 (MQ=255) gCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGACGTTCGCGAATCa > 1:3416583/1‑50 (MQ=255) ccTTCATCTTCTGACCAGTTCCAGTAGTAATCGCTGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGacac < 1:3782329/71‑1 (MQ=255) cTTCATCTTCTGACCAGTTCCAGTAGTAATCGCTGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGacac < 1:3777918/70‑1 (MQ=255) ttCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTcgcg < 1:879844/42‑1 (MQ=255) ttCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTcgcg < 1:304555/42‑1 (MQ=255) ttCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTc < 1:623332/39‑1 (MQ=255) cATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATc > 1:1049008/1‑44 (MQ=255) cATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCt > 1:288989/1‑71 (MQ=255) cATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCt > 1:2540663/1‑71 (MQ=255) aTCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCtt > 1:1599442/1‑71 (MQ=255) cttctGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCtttt > 1:8107/1‑71 (MQ=255) ttctGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCtttt > 1:1217471/1‑70 (MQ=255) ctGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCtttttt < 1:272888/70‑1 (MQ=255) tGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCa > 1:1013766/1‑71 (MQ=255) tGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCa > 1:2131126/1‑71 (MQ=255) tGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCa > 1:2156869/1‑71 (MQ=255) cAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGc < 1:3118069/70‑1 (MQ=255) aGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCt < 1:3568376/71‑1 (MQ=255) aGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCt < 1:2730330/71‑1 (MQ=255) ttCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTg > 1:2482827/1‑41 (MQ=255) ttCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTg > 1:266128/1‑41 (MQ=255) ttCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACg < 1:485366/66‑1 (MQ=255) ccAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCtttttt > 1:34819/1‑60 (MQ=255) aTCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCTTGCGCATCGCta > 1:2095056/1‑70 (MQ=255) aTCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCTTGCGCATCGCta > 1:577868/1‑70 (MQ=255) gcggcgTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCTTGCGCATCGCTATaa < 1:3739288/70‑1 (MQ=255) ggcgTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCTTGCGCATCGCTATAAAc < 1:3260098/70‑1 (MQ=255) gcgTTCGCGAATCAGGAGCACCTGCAAGCCCGACACGGCttt > 1:392478/1‑42 (MQ=255) | AGATCTTTTTGATCAAACTGTGACTGCCAGCCTTCATCTTCTGACCAGTTCCAGTAGTAATCGCGGCGTTCGCGAATCAGGCGCACCTGCAAGCCCGACACGGCTTTTTTCACGCCTTGCGCATCGCTATAAAC > minE/1682890‑1683023 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |