Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,763,636 A→G 30.4% noncoding (208/1542 nt) rrsG ← 16S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,763,6360AG30.4% 62.9 / 36.8 46noncoding (208/1542 nt)rrsG16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (18/14);  new base G (7/7);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 7.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCAT  >  minE/1763567‑1763705
                                                                     |                                                                     
gCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAg                                                                      >  1:3119249/1‑71 (MQ=255)
  cTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGt                                                                    <  1:1931852/71‑1 (MQ=255)
  cTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGt                                                                    <  1:247328/71‑1 (MQ=255)
    tGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTc                                                                   >  1:1946798/1‑70 (MQ=255)
         aGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCct                                                              <  1:2572956/70‑1 (MQ=255)
         aGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCct                                                              <  1:1429816/70‑1 (MQ=255)
         aGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCctc                                                             <  1:3464860/71‑1 (MQ=39)
          gCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCctct                                                            >  1:3175044/1‑71 (MQ=255)
          gCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCctct                                                            <  1:932720/71‑1 (MQ=39)
           gcGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCct                                                              <  1:737884/67‑1 (MQ=255)
           ccGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGAAAGAGGCCCGAGGGTCCCCCTCtt                                                           >  1:3830266/1‑71 (MQ=255)
            cGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCtt                                                           <  1:87605/70‑1 (MQ=40)
              ttACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTgg                                                        >  1:2102403/1‑71 (MQ=255)
              ttACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTgg                                                        >  1:807100/1‑71 (MQ=255)
              ttACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTgg                                                        >  1:188812/1‑71 (MQ=255)
               tACCCCACCAACAAGCTAATCCCATCTAGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGt                                                       >  1:655354/1‑71 (MQ=255)
                aCCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTc                                                      >  1:1336113/1‑71 (MQ=255)
                 ccccACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTc                                                      <  1:1916381/70‑1 (MQ=255)
                 ccccACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTc                                                      <  1:3396194/70‑1 (MQ=255)
                 ccccACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTc                                                      <  1:3751772/70‑1 (MQ=255)
                  cccACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCtt                                                    <  1:238712/71‑1 (MQ=39)
                    caccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGaa                                                                       >  1:747401/1‑50 (MQ=255)
                    caccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGaa                                                                       >  1:3538147/1‑50 (MQ=255)
                    caccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGc                                                  >  1:2238180/1‑71 (MQ=39)
                    caccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGc                                                  <  1:2336948/71‑1 (MQ=39)
                      ccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGa                                                <  1:2066386/71‑1 (MQ=39)
                      ccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGa                                                >  1:2263275/1‑71 (MQ=39)
                         acaaGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTCTTGCGAcgt                                             <  1:652546/71‑3 (MQ=9)
                         acaaGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTCTTGCGAcgt                                             >  1:2451211/1‑69 (MQ=9)
                         acaaGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACAt                                             <  1:1219273/71‑1 (MQ=38)
                            aGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTAt                                          >  1:2555676/1‑71 (MQ=34)
                            aGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTAt                                          >  1:720214/1‑71 (MQ=34)
                            aGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTAt                                          >  1:1578920/1‑71 (MQ=34)
                            aGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTAt                                          >  1:3429055/1‑71 (MQ=34)
                             gCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATg                                         <  1:2304342/71‑1 (MQ=34)
                                 aTCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCgg                                      >  1:2726603/1‑70 (MQ=35)
                                 aTCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCgg                                      >  1:2866687/1‑70 (MQ=35)
                                         tGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTa                             >  1:1078427/1‑71 (MQ=34)
                                           ggCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTAc                            <  1:1160472/70‑1 (MQ=35)
                                                 tCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATg                                         >  1:1852161/1‑51 (MQ=35)
                                                  ccGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTc                      >  1:1267192/1‑69 (MQ=35)
                                                           agagGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGtt              >  1:2070654/1‑68 (MQ=35)
                                                                 ccGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTc     >  1:143632/1‑71 (MQ=34)
                                                                 ccGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTc     <  1:1337968/71‑1 (MQ=34)
                                                                  cGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGt                         <  1:1740502/50‑1 (MQ=38)
                                                                  cGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTc     >  1:3642151/1‑70 (MQ=35)
                                                                  cGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTc     >  1:3316352/1‑70 (MQ=35)
                                                                   gAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCagtag                <  1:187754/58‑1 (MQ=35)
                                                                    aaGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCAt  <  1:3719976/71‑1 (MQ=34)
                                                                    aaGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCAt  <  1:1276133/71‑1 (MQ=34)
                                                                    aaGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCAt  <  1:290337/71‑1 (MQ=34)
                                                                    aaGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCAt  <  1:2754615/71‑1 (MQ=34)
                                                                     aGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCAt  >  1:2314574/1‑70 (MQ=35)
                                                                     |                                                                     
GCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCAT  >  minE/1763567‑1763705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑