| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,896,687 | C→A | 6.7% | Q137K (CAG→AAG) | argA → | fused acetylglutamate kinase homolog (inactive) and amino acid N‑acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,896,687 | 0 | C | A | 6.7% | 249.4 / 2.1 | 74 | Q137K (CAG→AAG) | argA | fused acetylglutamate kinase homolog (inactive) and amino acid N‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (30/39); new base A (3/2); total (33/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
ATGAGTCTCAATAACACGCCGCTGCAGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATTGATGAAGA > minE/1896618‑1896754 | aTGAGTCTCAATAACACGCCGCTGCAGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCa < 1:1672968/71‑1 (MQ=255)aTGAGTCTCAATAACACGCCGCTGCAGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCa < 1:657932/71‑1 (MQ=255)aTGAGTCTCAATAACACGCCGCTGCAGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCa < 1:1386395/71‑1 (MQ=255) aGTCTCAATAACACGCCGCTGCAGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCa < 1:782486/68‑1 (MQ=255) gTCTCAATAACACGCCGCTGCAGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCg < 1:3405875/71‑1 (MQ=255) tcAATAACACGCCGCTGCAGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCtt > 1:1420177/1‑70 (MQ=255) tcAATAACACGCCGCTGCAGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTg > 1:1745051/1‑71 (MQ=255) aaTAACACGCCGCTGCAGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTggg > 1:3177813/1‑71 (MQ=255) aaTAACACGCCGCTGCAGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTggg > 1:519906/1‑71 (MQ=255) aaTAACACGCCGCTGCAGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTgg < 1:331773/70‑1 (MQ=255) aaTAACACGCCGCTGCAGGGCGCGCATATCAACGGCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTgg < 1:1989604/70‑1 (MQ=255) aaTAACACGCCGCTGCACGGCGCGCATATCAACGTCGGCAGTGGCAATTTTATTATTGCCCAGCCGCt < 1:258729/68‑1 (MQ=255) aTAACACGCCGCTGCAGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTgg < 1:1897323/69‑1 (MQ=255) cgccgcTGCAGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCg > 1:2193453/1‑69 (MQ=255) cgccgcTGCAGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGAt < 1:2954453/71‑1 (MQ=255) cgccgcTGCAGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGAt < 1:2511301/71‑1 (MQ=255) cgccgcTGCAGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGAt < 1:1635748/71‑1 (MQ=255) cgccgcTGCAGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGAt < 1:1640043/71‑1 (MQ=255) cgccgcTGCAGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGAt < 1:2084319/71‑1 (MQ=255) cgccgcTGCAGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGAt < 1:350496/71‑1 (MQ=255) gccgcTGCAGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATg < 1:2799004/71‑1 (MQ=255) ccgcTGCAGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGa < 1:102830/71‑1 (MQ=255) cgcTGCAGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGAc < 1:1013254/71‑1 (MQ=255) cgcTGCAGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGAc > 1:3511405/1‑71 (MQ=255) aGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTg < 1:1230659/71‑1 (MQ=255) gggCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTgg < 1:2679397/71‑1 (MQ=255) gggCGCGCATATCAACGTCGTAAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTg > 1:637535/1‑70 (MQ=255) tatCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGcc < 1:3246320/71‑1 (MQ=255) tatCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGcc < 1:3064771/71‑1 (MQ=255) tatCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGc > 1:1639539/1‑70 (MQ=255) tatCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGc > 1:3244004/1‑70 (MQ=255) tatCAACGTCGTCAGTGGCAATTTTATTATTGCCAAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGcc < 1:3166388/71‑1 (MQ=255) atCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCa > 1:410407/1‑71 (MQ=255) tCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGa > 1:3370975/1‑52 (MQ=255) tCAACGTCGTCAGTGGCAATTTTATTATTGCCAAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCa > 1:3189563/1‑70 (MQ=255) tcgtcAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTgg < 1:1859702/54‑1 (MQ=255) tcgtcAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTgg < 1:1135110/54‑1 (MQ=255) tcgtcAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTgg < 1:1687821/54‑1 (MQ=255) cgtcAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGc > 1:676695/1‑71 (MQ=255) gTGGCAATTTTATTATTGCCCAGCCTCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCg < 1:3257915/67‑1 (MQ=255) gTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATc > 1:2780927/1‑71 (MQ=255) gTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATc > 1:1705933/1‑71 (MQ=255) gTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATc > 1:1685372/1‑71 (MQ=255) gTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATc > 1:2869013/1‑71 (MQ=255) gTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATc > 1:1328154/1‑71 (MQ=255) gTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGAGTGGATTACTGCCATAGCGGGCGTATc > 1:3787333/1‑71 (MQ=255) tGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGAt < 1:110834/37‑1 (MQ=255) tGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGAt < 1:157987/37‑1 (MQ=255) tGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATAACGGCGTg < 1:1830937/46‑1 (MQ=255) gCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACgg > 1:1222851/1‑40 (MQ=255) gCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAg < 1:2592870/58‑1 (MQ=255) gCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCcg > 1:1904245/1‑70 (MQ=255) gCAATTTTATTATTGCCCAGCCGCTGGGCGTCAATGACGGCGTGGATTACTGCCATAGCGGGCGTATCcg > 1:3115785/1‑70 (MQ=255) aaTTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCcggcg > 1:3800708/1‑71 (MQ=255) ttttATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATc < 1:655031/64‑1 (MQ=255) tttATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTg < 1:2781310/39‑1 (MQ=255) tttATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTg < 1:580499/39‑1 (MQ=255) tttATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGAtt < 1:3079995/43‑1 (MQ=255) ttattaTTGCCCAGCCGCTGGGCGTCGATGACGGCg > 1:2959315/1‑36 (MQ=255) ttattaTTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGAtt > 1:3538786/1‑71 (MQ=255) tattatTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGa > 1:1258497/1‑39 (MQ=255) tattatTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGa > 1:3120243/1‑39 (MQ=255) tattatTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTAc > 1:729762/1‑43 (MQ=255) tattatTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTAc > 1:3299620/1‑43 (MQ=255) tattatTGCCAAGCCGCTGGGCGTCGATGACGGCGTGGa > 1:1967671/1‑39 (MQ=255) tattatTGCCAAGCCGCTGGGCGTCGATGACGGCGTGGa > 1:2346488/1‑39 (MQ=255) tattatTGCCAAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCAATAGCGGGCGTATCCGGCGGAtt < 1:1923598/70‑1 (MQ=255) ttattGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATtgat < 1:1106472/71‑1 (MQ=255) ttattGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATtgat < 1:1375855/71‑1 (MQ=255) tattGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATtgat > 1:2405043/1‑70 (MQ=255) attGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATtgatg < 1:486732/70‑1 (MQ=255) attGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATtgat > 1:876872/1‑69 (MQ=255) ttGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATTGATGaa < 1:3483801/71‑1 (MQ=255) tGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATtgatg < 1:3428560/68‑1 (MQ=255) cccAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATtgatg < 1:3518767/66‑1 (MQ=255) cccAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATtgatg < 1:678444/66‑1 (MQ=255) cccAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATTGATGAAGa < 1:3654311/70‑1 (MQ=255) cccAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATTGATGAAGa < 1:2631864/70‑1 (MQ=255) cccAGCCGCTGGCCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATtgatg < 1:1342165/66‑1 (MQ=255) cAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATtgatga > 1:3715752/1‑65 (MQ=255) cAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATTGATGaa > 1:3019379/1‑66 (MQ=255) | ATGAGTCTCAATAACACGCCGCTGCAGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATTGATGAAGA > minE/1896618‑1896754 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |