| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 214,925 | A→C | 55.5% | D12A (GAT→GCT) | dnaQ → | DNA polymerase III epsilon subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 214,925 | 0 | A | C | 55.5% | 186.4 / 202.8 | 182 | D12A (GAT→GCT) | dnaQ | DNA polymerase III epsilon subunit |
| Reads supporting (aligned to +/- strand): ref base A (44/37); new base C (57/44); total (101/81) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 185 total aligned reads displayed.
TAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGG > minE/214860‑214991 | tAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGa > 1:344069/1‑66 (MQ=255)tAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATAc > 1:3165669/1‑69 (MQ=255)tAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCg < 1:1169068/71‑1 (MQ=255) aaaCGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGaa < 1:651895/71‑1 (MQ=255) aaaCGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGa > 1:1111766/1‑70 (MQ=255) aaaCGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGa < 1:3487274/70‑1 (MQ=255) aaaCGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGa > 1:2284414/1‑70 (MQ=255) actcAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAac > 1:323615/4‑70 (MQ=255) actcAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAac > 1:3754532/4‑70 (MQ=255) cAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAaccacc > 1:3828932/1‑71 (MQ=255) aGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg < 1:3568046/71‑1 (MQ=255) aGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCgg < 1:1604579/71‑1 (MQ=255) tCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGa < 1:2535304/60‑1 (MQ=255) cTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTAt > 1:2104254/1‑71 (MQ=255) cTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTAt > 1:2569026/1‑71 (MQ=255) cTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTAt > 1:2394724/1‑71 (MQ=255) tGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTAt > 1:951067/1‑70 (MQ=255) tGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTAt > 1:926086/1‑70 (MQ=255) tGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTAt > 1:212133/1‑70 (MQ=255) gACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCgca < 1:13803/58‑3 (MQ=255) gACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCgca < 1:75863/58‑3 (MQ=255) gACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATg > 1:1463658/1‑70 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATg < 1:961907/69‑1 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGaa > 1:2611657/1‑71 (MQ=255) aCATAAATGACCGCTATCAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGaa > 1:3060425/1‑71 (MQ=255) cATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAAc > 1:2267009/1‑71 (MQ=255) tAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCa < 1:629233/71‑1 (MQ=255) aaaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:2366385/1‑68 (MQ=255) aaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:3764344/1‑67 (MQ=255) aaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAg < 1:861723/70‑1 (MQ=255) aTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGAt > 1:1915870/1‑71 (MQ=255) aCCGCTATGAGCACT‑CAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGt > 1:3410912/1‑71 (MQ=255) ccGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATg < 1:1570897/59‑1 (MQ=255) ccGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATg < 1:1091951/59‑1 (MQ=255) cTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTgcg > 1:2430405/1‑71 (MQ=255) cTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTgcg > 1:476613/1‑71 (MQ=255) aTGAGCACTGCAATTACACGCCAGATCGTTCTCGa > 1:3162889/1‑35 (MQ=255) aTGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCa > 1:22319/1‑71 (MQ=255) aTGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCa > 1:3513056/1‑71 (MQ=255) gAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGa < 1:704247/53‑1 (MQ=255) gCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTAt > 1:3780218/1‑71 (MQ=255) aCTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGa < 1:3176102/71‑1 (MQ=255) aCTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGa < 1:2525916/71‑1 (MQ=255) tGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg < 1:1289852/42‑1 (MQ=255) gCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAgg > 1:26844/1‑71 (MQ=255) cAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAgg > 1:2062236/1‑70 (MQ=255) cAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc > 1:2793690/1‑71 (MQ=255) cAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc > 1:2805829/1‑71 (MQ=255) aTTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGa > 1:3846715/1‑49 (MQ=255) aTTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCa > 1:176094/1‑59 (MQ=255) ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATg < 1:1216136/41‑1 (MQ=38) ttACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCa < 1:2888039/46‑1 (MQ=255) ttACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCa < 1:1154206/46‑1 (MQ=255) ttACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCa < 1:2177354/46‑1 (MQ=255) ctACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATg < 1:2644892/40‑1 (MQ=38) tACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:814204/1‑70 (MQ=255) tACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:1680840/1‑70 (MQ=255) tACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTCGTGCGCACTATGAAGGCcac > 1:3072485/1‑70 (MQ=255) acacGCCAGATCGTTCTCGCTACCGCAACCACCgg > 1:1664280/1‑35 (MQ=38) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGa > 1:1106066/1‑46 (MQ=39) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTg > 1:2387973/1‑49 (MQ=39) acacGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGa > 1:387369/1‑46 (MQ=255) acacGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:281241/1‑69 (MQ=255) cacGCCAGATCGTTCTCGCTACCGCAACCACCGGTa < 1:2747909/36‑1 (MQ=37) cacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:3392773/1‑68 (MQ=255) cacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:981899/1‑68 (MQ=255) cacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:311043/1‑68 (MQ=255) cacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:2115310/1‑68 (MQ=255) cacGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACaa < 1:2711557/70‑1 (MQ=255) acGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGa < 1:2948754/71‑1 (MQ=255) cGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGAt < 1:3084819/71‑1 (MQ=255) cGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:2708166/1‑66 (MQ=255) cGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:3016273/1‑66 (MQ=255) gCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:1984919/57‑1 (MQ=255) gCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATc < 1:3291871/71‑1 (MQ=255) cAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGa > 1:2124365/1‑56 (MQ=255) aGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAt > 1:2495641/1‑70 (MQ=255) aGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt < 1:3777104/71‑1 (MQ=255) gATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCAc < 1:2979531/49‑1 (MQ=39) gATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCAc < 1:303488/49‑1 (MQ=39) gATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:1741491/1‑70 (MQ=255) gATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTgg < 1:973439/42‑1 (MQ=255) aTCGTTCTCGCTACCGCAACCACCGGTATGAACCa < 1:3404282/35‑1 (MQ=37) aTCGTTCTCGCTACCGCAACCACCGGTATGAACCa < 1:1327606/35‑1 (MQ=37) aTCGTTCTCGCTACCGCAACCACCGGTATGAACCa < 1:1897701/35‑1 (MQ=37) aTCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTga < 1:1720890/71‑1 (MQ=255) tCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTa > 1:1048496/1‑49 (MQ=39) tCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTga < 1:3629231/70‑1 (MQ=255) cGTTCTCGCTACCGCAACCACCGGTATGAACCAGa > 1:3338960/1‑35 (MQ=37) cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:100565/1‑67 (MQ=255) cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:2963051/1‑67 (MQ=255) cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:1657426/1‑67 (MQ=255) gTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGAt > 1:459079/1‑71 (MQ=255) gTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGAt < 1:1139455/71‑1 (MQ=255) ttCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTAt < 1:2047881/47‑1 (MQ=39) ttCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTAt < 1:1585866/47‑1 (MQ=39) ttCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTAt < 1:3171696/47‑1 (MQ=39) ctcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGa > 1:3040722/1‑47 (MQ=39) ctcGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:2056558/1‑63 (MQ=255) tcGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTgg > 1:364652/1‑70 (MQ=255) | TAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGG > minE/214860‑214991 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |