| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,970,934 | C→T | 8.0% | E658K (GAG→AAG) | speA ← | biosynthetic arginine decarboxylase, PLP‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,970,934 | 0 | C | T | 8.0% | 364.5 / 14.7 | 112 | E658K (GAG→AAG) | speA | biosynthetic arginine decarboxylase, PLP‑binding |
| Reads supporting (aligned to +/- strand): ref base C (47/56); new base T (5/4); total (52/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 114 total aligned reads displayed.
ATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCA > minE/1970864‑1971001 | aTTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTc < 1:2407047/71‑1 (MQ=255) tGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATc > 1:2021219/1‑71 (MQ=255) tCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCt < 1:342051/70‑1 (MQ=255) cacaATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCtt > 1:1506936/1‑70 (MQ=255) acaATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTc < 1:2988995/70‑1 (MQ=255) acaATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTc < 1:2340619/70‑1 (MQ=255) acaATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTc < 1:2662975/70‑1 (MQ=255) aaTAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTc > 1:790751/1‑68 (MQ=255) aTAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAg > 1:3044521/1‑70 (MQ=255) aTAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAg > 1:3696189/1‑70 (MQ=255) aTAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAg > 1:1469805/1‑70 (MQ=255) tAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTc < 1:3184821/66‑1 (MQ=255) tAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTc < 1:3551628/66‑1 (MQ=255) tAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGAt < 1:1281996/71‑1 (MQ=255) aaTTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGAt > 1:1675546/1‑69 (MQ=255) aTTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAg < 1:2242609/71‑1 (MQ=255) tgGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGtat < 1:3644119/70‑1 (MQ=255) cGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTc < 1:2420041/56‑1 (MQ=255) gATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCtt < 1:3040808/53‑1 (MQ=255) gATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCtt < 1:2117574/53‑1 (MQ=255) aTTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTa < 1:231975/71‑1 (MQ=255) aTTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTa < 1:1058271/71‑1 (MQ=255) tATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCtt < 1:2000543/50‑1 (MQ=255) tATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTAc > 1:2046323/1‑70 (MQ=255) tATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTAc > 1:2579035/1‑70 (MQ=255) aTCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACa < 1:1896854/70‑1 (MQ=255) aTCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACa < 1:3018315/70‑1 (MQ=255) aTCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACa > 1:3382546/1‑70 (MQ=255) aTCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACa > 1:2296689/1‑70 (MQ=255) tCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACaa < 1:3530000/70‑1 (MQ=255) tCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACaa < 1:3255953/70‑1 (MQ=255) aCCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTTATCTTGAAGATGAGtaa > 1:2332778/1‑57 (MQ=37) aCCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTTATATTGAAGATGAGtaa > 1:3217821/1‑57 (MQ=37) aCCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAAc < 1:535740/70‑1 (MQ=255) ccGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCt < 1:3031858/71‑1 (MQ=255) cGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCg < 1:3192478/61‑1 (MQ=255) gCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGc > 1:2295436/1‑71 (MQ=255) aaaTTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGa > 1:3379663/1‑45 (MQ=255) aaTTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATaa > 1:1348056/1‑53 (MQ=255) aaTTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATaa > 1:400234/1‑53 (MQ=255) aaTTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATaa > 1:341813/1‑53 (MQ=255) aaTTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTc > 1:2152490/1‑70 (MQ=255) aaTTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTc > 1:1006693/1‑70 (MQ=255) aaTTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGAAAAGTATAAACGTACAAACCTGCCt > 1:1234867/1‑69 (MQ=255) aaTTAAGAGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTc > 1:1445415/1‑70 (MQ=255) tAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAAc > 1:1497443/1‑71 (MQ=255) tAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAAc > 1:2719551/1‑71 (MQ=255) aaGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAAct > 1:3735861/1‑71 (MQ=255) aaGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAAct > 1:666345/1‑71 (MQ=255) aGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTACCTCGAActc < 1:623457/71‑1 (MQ=255) gCGGATTCAAGTAACACATGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAActct < 1:1861423/71‑1 (MQ=255) gCGGATTCAAGTAACACAGGACTTACTTATCTTGAAGAtga < 1:1312115/41‑3 (MQ=25) gCGGATTCAAGTAACACAGGACTTACTTATCTTGAAGAtga < 1:2310047/41‑3 (MQ=25) gCGGATTCAAGTAACACAGGACTTACTTATCTTGAAGAtga < 1:2306144/41‑3 (MQ=25) gCGGATTCAAGTAACACAGGACTTACTTATCTTGAAGAtga < 1:2283439/41‑3 (MQ=25) gCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCTAActct < 1:496470/71‑1 (MQ=255) gCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAActct < 1:1659772/71‑1 (MQ=255) gCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAActct < 1:3151293/71‑1 (MQ=255) gCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAActct > 1:3650840/1‑71 (MQ=255) ggATTCAAGTAACACAGGACTTACTTATCTTGAAGATGAGt > 1:2672116/1‑41 (MQ=21) ggATTCAAGTAACACAGGACTTACTTATCTTGAAGATGAGt > 1:1249756/1‑41 (MQ=21) ggATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTc < 1:461489/71‑1 (MQ=255) ggATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTc < 1:3001148/71‑1 (MQ=255) ggATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTc < 1:3807977/71‑1 (MQ=255) ggATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTc < 1:654640/71‑1 (MQ=255) ttCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAg > 1:3270314/1‑71 (MQ=255) ttCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAg > 1:3820288/1‑71 (MQ=255) ttCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAg > 1:3718727/1‑71 (MQ=255) ttCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAg > 1:2494551/1‑71 (MQ=255) ttCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAg > 1:1623354/1‑71 (MQ=255) aaGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCaa > 1:2019348/1‑67 (MQ=255) aaGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCaa > 1:824290/1‑67 (MQ=255) aaGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCaa > 1:1432688/1‑67 (MQ=255) aaGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCaa > 1:3567843/1‑67 (MQ=255) aaGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGaa > 1:1202950/1‑71 (MQ=255) aaGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGaa > 1:3811060/1‑71 (MQ=255) aaGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGa < 1:237469/70‑1 (MQ=255) aaGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGa < 1:3818586/70‑1 (MQ=255) aGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAAc < 1:3643259/71‑1 (MQ=255) aGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAAc < 1:2766523/71‑1 (MQ=255) aGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAAc < 1:229165/71‑1 (MQ=255) gTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCg < 1:3121767/55‑1 (MQ=255) tAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACtg > 1:201976/1‑71 (MQ=255) acacAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACtgtt > 1:2859732/1‑71 (MQ=255) acacAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACtgt > 1:1155473/1‑70 (MQ=255) acaGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCaa < 1:2281834/60‑1 (MQ=255) acaGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACtgttgt < 1:2356353/71‑1 (MQ=255) ggACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGTAACtgt < 1:240071/65‑1 (MQ=255) ggACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACtgt < 1:2316500/65‑1 (MQ=255) ggACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACtgt < 1:1174824/65‑1 (MQ=255) ggACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGttgttg > 1:1523663/1‑70 (MQ=255) gACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACtgtt < 1:507394/65‑1 (MQ=255) cTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGt < 1:1584352/71‑1 (MQ=255) cTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGt < 1:3768153/71‑1 (MQ=255) ttACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGt < 1:214826/70‑1 (MQ=255) tACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCg < 1:2809447/42‑1 (MQ=255) tACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCg < 1:1366926/42‑1 (MQ=255) aCTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGaa > 1:2675561/1‑55 (MQ=255) cTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCa < 1:1960283/70‑1 (MQ=255) cATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCg > 1:1531341/1‑38 (MQ=255) | ATTTGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTTCA > minE/1970864‑1971001 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |