Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,065,298 T→C 7.7% D513D (GAT→GAC rpoD → RNA polymerase, sigma 70 (sigma D) factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,065,2980TC 7.7% 224.6 / 9.2 78D513D (GAT→GACrpoDRNA polymerase, sigma 70 (sigma D) factor
Reads supporting (aligned to +/- strand):  ref base T (33/39);  new base C (3/3);  total (36/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

CGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACC  >  minE/2065228‑2065367
                                                                      |                                                                     
cGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGtgat                                                                       <  1:242448/71‑1 (MQ=255)
cGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGtgat                                                                       <  1:2061263/71‑1 (MQ=255)
cGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGtcat                                                                       <  1:1680481/71‑1 (MQ=255)
  gAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGtgatga                                                                     >  1:2699988/1‑71 (MQ=255)
    aGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGtgatgatg                                                                   <  1:2613921/71‑1 (MQ=255)
       cAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGaa                                                                 <  1:901134/70‑1 (MQ=255)
        aaGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGa                                                               <  1:2494898/71‑1 (MQ=255)
        aaGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGa                                                               <  1:588724/71‑1 (MQ=255)
        aaGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGa                                                               <  1:1439913/71‑1 (MQ=255)
        aaGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATCGAAACGCCGATCGGTGATGATGAAGa                                                               <  1:3658777/71‑1 (MQ=255)
           aTCCGCAAAGTGCTTAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTc                                                            <  1:234151/71‑1 (MQ=255)
            tCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCg                                                           >  1:3768927/1‑71 (MQ=255)
             ccGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGtgat                                                                       <  1:1035130/58‑1 (MQ=255)
             ccGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGtgat                                                                       <  1:71414/58‑1 (MQ=255)
             ccGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGtgat                                                                       <  1:1999267/58‑1 (MQ=255)
             ccGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCg                                                           >  1:3117410/1‑70 (MQ=255)
                cAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGtgatgat                                                                    <  1:3157864/58‑1 (MQ=255)
                cAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGtgatgat                                                                    <  1:2523941/58‑1 (MQ=255)
                cAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGtgatgat                                                                    <  1:1916620/58‑1 (MQ=255)
                cAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGtgatgat                                                                    <  1:849005/58‑1 (MQ=255)
                cAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGa                                                               <  1:2937828/63‑1 (MQ=255)
                cAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGa                                                               <  1:2338090/63‑1 (MQ=255)
                cAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATc                                                       >  1:1686849/1‑71 (MQ=255)
                cAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATc                                                       >  1:1205073/1‑71 (MQ=255)
                cAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATc                                                       >  1:1210391/1‑71 (MQ=255)
                  aaGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGtgatgatga                                                                  >  1:2990992/1‑58 (MQ=255)
                  aaGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTg                                                     <  1:1795045/71‑1 (MQ=255)
                       cTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGa                                                >  1:2247422/1‑71 (MQ=255)
                       cTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGa                                                >  1:2095758/1‑71 (MQ=255)
                            gATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCa                                           >  1:786259/1‑71 (MQ=255)
                             aTCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCAt                                          >  1:1103403/1‑71 (MQ=255)
                              tCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATc                                         <  1:324867/71‑1 (MQ=255)
                               cGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGtgatga                                                                     >  1:102777/1‑42 (MQ=255)
                               cGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGa                                                               >  1:2903613/1‑48 (MQ=255)
                               cGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGa                                                               >  1:1913079/1‑48 (MQ=255)
                               cGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGAttt                                             >  1:2743747/1‑66 (MQ=255)
                               cGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATc                                         <  1:3082065/70‑1 (MQ=255)
                                gCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGTGGATTTCATCg                                        <  1:1446943/70‑1 (MQ=255)
                                gCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCg                                        <  1:2603551/70‑1 (MQ=255)
                                 ccAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCGTCGAg                                      <  1:766822/71‑1 (MQ=255)
                                 ccAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg                                      <  1:3207406/71‑1 (MQ=255)
                                 ccAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg                                      <  1:3161012/71‑1 (MQ=255)
                                 ccAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg                                      <  1:3853033/71‑1 (MQ=255)
                                  cAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg                                      <  1:1812293/70‑1 (MQ=255)
                                   aaaGAGCCTATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa                                    <  1:1408696/71‑1 (MQ=255)
                                   aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCg                                        <  1:1088949/67‑1 (MQ=255)
                                   aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGATGa                                    <  1:3044053/71‑1 (MQ=255)
                                   aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa                                    <  1:2509446/71‑1 (MQ=255)
                                   aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa                                    <  1:713968/71‑1 (MQ=255)
                                   aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa                                    <  1:2737500/71‑1 (MQ=255)
                                   aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa                                    <  1:3306752/71‑1 (MQ=255)
                                   aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa                                    <  1:1104467/71‑1 (MQ=255)
                                   aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa                                    >  1:1295762/1‑71 (MQ=255)
                                   aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa                                    <  1:900319/71‑1 (MQ=255)
                                   aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa                                    <  1:331376/71‑1 (MQ=255)
                                   aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGACTTCATCGAGGa                                    <  1:984003/71‑1 (MQ=255)
                                    aaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa                                    >  1:2393926/1‑70 (MQ=255)
                                    aaGAGCCAATCTCCATGGAAACGCCGATCGGTGACGACGACGATTCGCATCTGGGCGATTTTATCGAGGac                                   <  1:480083/71‑2 (MQ=255)
                                        gCCAATCTCCATGGAAACGCCGATCGGTGACGACGACGATTCGCAt                                                        <  1:1307042/46‑1 (MQ=37)
                                        gCCAATCTCCATGGAAACGCCGATCGGTGACGACGACGATTCGCAt                                                        <  1:138186/46‑1 (MQ=37)
                                          cAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATaccacc                             <  1:333280/71‑1 (MQ=255)
                                          cAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATaccacc                             <  1:3323347/71‑1 (MQ=255)
                                          cAATCTCCATGGAAACGCCGATCGGTGACGACGACGATTCGCATCt                                                      >  1:2480247/1‑46 (MQ=37)
                                          cAATCTCCATGGAAACGCCGATCGGTGACGACGACGATTCGCATCt                                                      >  1:2365259/1‑46 (MQ=37)
                                          cAATCTCCATGGAAACGCCGATCGGTGACGACGACGATTCGCATCt                                                      >  1:1852010/1‑46 (MQ=37)
                                           aaTCTCCATGGAAACGCCGATCGGTGATGATGAAGa                                                               >  1:1809978/1‑36 (MQ=255)
                                           aaTCTCCATGGAAACGCCGATCGGTGATGATGAAGa                                                               >  1:2143287/1‑36 (MQ=255)
                                           aaTCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa                                    >  1:56116/1‑63 (MQ=255)
                                            aTCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCt                           <  1:811387/71‑1 (MQ=255)
                                            aTCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCt                           >  1:2377553/1‑71 (MQ=255)
                                             tctcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa                                    <  1:1583573/61‑1 (MQ=255)
                                               tcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTc                          >  1:1692286/1‑69 (MQ=255)
                                               tcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTc                          >  1:1658713/1‑69 (MQ=255)
                                               tcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTc                          >  1:1874854/1‑69 (MQ=255)
                                               tcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTc                          >  1:89992/1‑69 (MQ=255)
                                                 cATGGAAACGCCGATCGGTGATTATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCg                         <  1:226399/68‑1 (MQ=255)
                                                 cATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTc                          <  1:1050330/67‑1 (MQ=255)
                                                 cATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGa                        <  1:2949382/69‑1 (MQ=255)
                                                 cATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACAACCCTCg                         <  1:3094172/68‑1 (MQ=255)
                                                   tGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTg                    >  1:1281021/1‑71 (MQ=255)
                                                    ggAAACGCCGATCGGTGATGATGAAGATTCTCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGc                   >  1:2570866/1‑71 (MQ=255)
                                                     gAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg                                      >  1:483461/1‑51 (MQ=255)
                                                     gAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg                                      >  1:180402/1‑51 (MQ=255)
                                                      aaaCGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGAt                                               >  1:971757/1‑41 (MQ=255)
                                                      aaaCGCCGATCGGTGATGATGAAGATTCGCATATGGGGGAt                                               >  1:3202337/1‑41 (MQ=255)
                                                        aCGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCt               <  1:3239428/71‑1 (MQ=255)
                                                         cGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCt               >  1:871820/1‑70 (MQ=255)
                                                              aTCGGTGATGATGAAGATTCGCATCTGGGGTATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTc         >  1:2650316/1‑71 (MQ=255)
                                                              aTCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTc         >  1:1996875/1‑71 (MQ=255)
                                                              aTCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTc         <  1:1306616/71‑1 (MQ=255)
                                                              aTCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTc         >  1:2799259/1‑71 (MQ=255)
                                                               tCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACTACCCTCGAGCTGCCGCTGGATTCt        >  1:3860941/1‑71 (MQ=255)
                                                                     atgatgaAGATTCGCATCTGGGGGATTTCATCGAGGa                                    <  1:1993727/37‑1 (MQ=255)
                                                                     atgatgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGacc  <  1:3830728/71‑1 (MQ=255)
                                                                      |                                                                     
CGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACC  >  minE/2065228‑2065367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: