| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,065,301 | T→C | 7.7% | D514D (GAT→GAC) | rpoD → | RNA polymerase, sigma 70 (sigma D) factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,065,301 | 0 | T | C | 7.7% | 223.3 / 9.2 | 78 | D514D (GAT→GAC) | rpoD | RNA polymerase, sigma 70 (sigma D) factor |
| Reads supporting (aligned to +/- strand): ref base T (33/39); new base C (3/3); total (36/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
AGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCAC > minE/2065232‑2065369 | aGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGtgatgatg < 1:2613921/71‑1 (MQ=255) cAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGaa < 1:901134/70‑1 (MQ=255) aaGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGa < 1:2494898/71‑1 (MQ=255) aaGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGa < 1:1439913/71‑1 (MQ=255) aaGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGa < 1:588724/71‑1 (MQ=255) aaGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATCGAAACGCCGATCGGTGATGATGAAGa < 1:3658777/71‑1 (MQ=255) aTCCGCAAAGTGCTTAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTc < 1:234151/71‑1 (MQ=255) tCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCg > 1:3768927/1‑71 (MQ=255) ccGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCg > 1:3117410/1‑70 (MQ=255) cAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGtgatgat < 1:2523941/58‑1 (MQ=255) cAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGtgatgat < 1:1916620/58‑1 (MQ=255) cAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGtgatgat < 1:849005/58‑1 (MQ=255) cAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGtgatgat < 1:3157864/58‑1 (MQ=255) cAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGa < 1:2338090/63‑1 (MQ=255) cAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGa < 1:2937828/63‑1 (MQ=255) cAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATc > 1:1686849/1‑71 (MQ=255) cAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATc > 1:1210391/1‑71 (MQ=255) cAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATc > 1:1205073/1‑71 (MQ=255) aaGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGtgatgatga > 1:2990992/1‑58 (MQ=255) aaGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTg < 1:1795045/71‑1 (MQ=255) cTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGa > 1:2247422/1‑71 (MQ=255) cTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGa > 1:2095758/1‑71 (MQ=255) gATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCa > 1:786259/1‑71 (MQ=255) aTCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCAt > 1:1103403/1‑71 (MQ=255) tCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATc < 1:324867/71‑1 (MQ=255) cGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGa > 1:1913079/1‑48 (MQ=255) cGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGa > 1:2903613/1‑48 (MQ=255) cGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGAttt > 1:2743747/1‑66 (MQ=255) cGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATc < 1:3082065/70‑1 (MQ=255) gCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGTGGATTTCATCg < 1:1446943/70‑1 (MQ=255) gCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCg < 1:2603551/70‑1 (MQ=255) ccAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCGTCGAg < 1:766822/71‑1 (MQ=255) ccAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg < 1:3853033/71‑1 (MQ=255) ccAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg < 1:3161012/71‑1 (MQ=255) ccAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg < 1:3207406/71‑1 (MQ=255) cAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg < 1:1812293/70‑1 (MQ=255) aaaGAGCCTATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa < 1:1408696/71‑1 (MQ=255) aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCg < 1:1088949/67‑1 (MQ=255) aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGATGa < 1:3044053/71‑1 (MQ=255) aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa < 1:900319/71‑1 (MQ=255) aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa < 1:1104467/71‑1 (MQ=255) aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa < 1:2509446/71‑1 (MQ=255) aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:1295762/1‑71 (MQ=255) aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa < 1:713968/71‑1 (MQ=255) aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa < 1:331376/71‑1 (MQ=255) aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa < 1:3306752/71‑1 (MQ=255) aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa < 1:2737500/71‑1 (MQ=255) aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGACTTCATCGAGGa < 1:984003/71‑1 (MQ=255) aaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:2393926/1‑70 (MQ=255) aaGAGCCAATCTCCATGGAAACGCCGATCGGTGACGACGACGATTCGCATCTGGGCGATTTTATCGAGGac < 1:480083/71‑2 (MQ=255) gCCAATCTCCATGGAAACGCCGATCGGTGACGACGACGATTCGCAt < 1:1307042/46‑1 (MQ=37) gCCAATCTCCATGGAAACGCCGATCGGTGACGACGACGATTCGCAt < 1:138186/46‑1 (MQ=37) cAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATaccacc < 1:333280/71‑1 (MQ=255) cAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATaccacc < 1:3323347/71‑1 (MQ=255) cAATCTCCATGGAAACGCCGATCGGTGACGACGACGATTCGCATCt > 1:2480247/1‑46 (MQ=37) cAATCTCCATGGAAACGCCGATCGGTGACGACGACGATTCGCATCt > 1:2365259/1‑46 (MQ=37) cAATCTCCATGGAAACGCCGATCGGTGACGACGACGATTCGCATCt > 1:1852010/1‑46 (MQ=37) aaTCTCCATGGAAACGCCGATCGGTGATGATGAAGa > 1:1809978/1‑36 (MQ=255) aaTCTCCATGGAAACGCCGATCGGTGATGATGAAGa > 1:2143287/1‑36 (MQ=255) aaTCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:56116/1‑63 (MQ=255) aTCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCt > 1:2377553/1‑71 (MQ=255) aTCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCt < 1:811387/71‑1 (MQ=255) tctcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa < 1:1583573/61‑1 (MQ=255) tcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTc > 1:1692286/1‑69 (MQ=255) tcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTc > 1:1658713/1‑69 (MQ=255) tcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTc > 1:89992/1‑69 (MQ=255) tcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTc > 1:1874854/1‑69 (MQ=255) cATGGAAACGCCGATCGGTGATTATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCg < 1:226399/68‑1 (MQ=255) cATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTc < 1:1050330/67‑1 (MQ=255) cATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGa < 1:2949382/69‑1 (MQ=255) cATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACAACCCTCg < 1:3094172/68‑1 (MQ=255) tGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTg > 1:1281021/1‑71 (MQ=255) ggAAACGCCGATCGGTGATGATGAAGATTCTCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGc > 1:2570866/1‑71 (MQ=255) gAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg > 1:180402/1‑51 (MQ=255) gAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg > 1:483461/1‑51 (MQ=255) aaaCGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGAt > 1:971757/1‑41 (MQ=255) aaaCGCCGATCGGTGATGATGAAGATTCGCATATGGGGGAt > 1:3202337/1‑41 (MQ=255) aCGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCt < 1:3239428/71‑1 (MQ=255) cGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCt > 1:871820/1‑70 (MQ=255) aTCGGTGATGATGAAGATTCGCATCTGGGGTATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTc > 1:2650316/1‑71 (MQ=255) aTCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTc < 1:1306616/71‑1 (MQ=255) aTCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTc > 1:1996875/1‑71 (MQ=255) aTCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTc > 1:2799259/1‑71 (MQ=255) tCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACTACCCTCGAGCTGCCGCTGGATTCt > 1:3860941/1‑71 (MQ=255) atgatgaAGATTCGCATCTGGGGGATTTCATCGAGGa < 1:1993727/37‑1 (MQ=255) atgatgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGacc < 1:3830728/71‑1 (MQ=255) gatgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGaccac > 1:302534/1‑71 (MQ=255) gatgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGaccac > 1:2269335/1‑71 (MQ=255) gatgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGaccac > 1:3711138/1‑71 (MQ=255) gatgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGaccac > 1:2049780/1‑71 (MQ=255) gatgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGaccac > 1:1846558/1‑71 (MQ=255) tgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTc < 1:556230/43‑1 (MQ=255) | AGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCAC > minE/2065232‑2065369 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |