| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 317,968 | C→T | 13.7% | T988T (ACC→ACT) | kefA → | fused mechanosensitive channel proteins |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 317,968 | 0 | C | T | 13.7% | 201.2 / 20.6 | 73 | T988T (ACC→ACT) | kefA | fused mechanosensitive channel proteins |
| Reads supporting (aligned to +/- strand): ref base C (22/41); new base T (5/5); total (27/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
TTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGG > minE/317901‑318032 | ttCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAg > 1:136013/1‑71 (MQ=255) tCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGc > 1:3052046/1‑71 (MQ=255) cGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTg < 1:540896/71‑1 (MQ=255) gACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGa < 1:1551013/71‑1 (MQ=255) aCAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGa < 1:3769050/70‑1 (MQ=255) aCAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGAt > 1:982414/1‑71 (MQ=255) aaCGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATc > 1:236886/1‑70 (MQ=255) aaCGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATc > 1:3567456/1‑70 (MQ=255) aaCGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCa < 1:1691798/71‑1 (MQ=255) aaCGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCa < 1:2861193/71‑1 (MQ=255) aaCGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCa < 1:2655166/71‑1 (MQ=255) gATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACt > 1:3646348/1‑71 (MQ=255) aTTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGa < 1:2952219/64‑1 (MQ=255) tACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATc > 1:721193/1‑64 (MQ=255) gATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGtt < 1:3791531/71‑1 (MQ=255) gATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGtt < 1:892659/71‑1 (MQ=255) gATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGtt < 1:2902279/71‑1 (MQ=255) cGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTtgact < 1:28883/70‑1 (MQ=255) cGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTtgact < 1:2707052/70‑1 (MQ=255) gATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTtgactga < 1:842479/71‑1 (MQ=255) cGCAAAGAAGTGATCATCCCGAGCAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGacac > 1:2055083/1‑71 (MQ=255) cGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGacac > 1:1625001/1‑71 (MQ=255) gCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCg < 1:772342/59‑1 (MQ=255) gCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGacac < 1:2370406/70‑1 (MQ=255) gCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACact < 1:2662157/71‑1 (MQ=255) aagaagTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACactact < 1:1817915/71‑1 (MQ=255) agaagTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACActacta < 1:1489916/71‑1 (MQ=255) agaagTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACActacta < 1:1207927/71‑1 (MQ=255) gaatTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACtactac > 1:1663531/1‑71 (MQ=255) gaagTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACtactac < 1:2180557/71‑1 (MQ=255) tGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTg < 1:2185870/38‑1 (MQ=255) tGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTg < 1:863613/38‑1 (MQ=255) tGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTg < 1:1048952/38‑1 (MQ=255) tGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAAc < 1:365970/44‑1 (MQ=255) tGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAAc < 1:1586982/44‑1 (MQ=255) gATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTc > 1:62874/1‑71 (MQ=255) atcatcCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCt < 1:771293/71‑1 (MQ=255) atcatcCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCt < 1:600723/71‑1 (MQ=255) atcatcCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCt < 1:1278999/71‑1 (MQ=255) atcatcCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCt < 1:1350921/71‑1 (MQ=255) atcatcCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCt < 1:472127/71‑1 (MQ=255) atcatcCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCt < 1:2243686/71‑1 (MQ=255) atcatcCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCt < 1:1993406/71‑1 (MQ=255) tcatcCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCt < 1:602904/70‑1 (MQ=255) tcatcCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTg < 1:1695365/71‑1 (MQ=255) tcatcCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTg < 1:544609/71‑1 (MQ=255) catcCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTgg > 1:2436567/1‑71 (MQ=255) tcCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTg > 1:1163104/1‑68 (MQ=255) tcCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTg > 1:1983830/1‑68 (MQ=255) tcCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGt > 1:750518/1‑70 (MQ=255) tcCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGt > 1:243315/1‑70 (MQ=255) tcCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGt > 1:1909221/1‑70 (MQ=255) tcCCGAACAAAGCGTTTCTTACCGAGCGTCTGATCa > 1:2966586/1‑36 (MQ=255) ccGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGa > 1:3171752/1‑70 (MQ=255) gAACAAAGCGTTTGTTACCGAGCGTCTGTTCAACTGGTCg < 1:1116395/40‑1 (MQ=255) gAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATcc < 1:896511/71‑1 (MQ=255) aaCAAAGCGTTTGTTACTGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCg > 1:2798559/1‑71 (MQ=255) aaGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGt > 1:1446954/1‑56 (MQ=255) aaGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGt > 1:3215323/1‑56 (MQ=255) aaGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGtctc < 1:587019/71‑1 (MQ=255) aaGCGTTTGTTACCGAGCGTCCGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGtctc < 1:2360171/71‑1 (MQ=255) gCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCg < 1:1875271/65‑1 (MQ=255) tttGTTACTGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGt < 1:562270/71‑1 (MQ=255) tttGTTACTGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGt < 1:1550928/71‑1 (MQ=255) tttGTTACTGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGt < 1:979527/71‑1 (MQ=255) tttGTTACTGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGt < 1:1769384/71‑1 (MQ=255) tttGTTACTGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGt < 1:2363452/71‑1 (MQ=255) tGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTgg > 1:1604103/1‑71 (MQ=255) tGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTg < 1:3266707/70‑1 (MQ=255) tACTGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGtct > 1:432066/1‑60 (MQ=255) tACTGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGtct > 1:207416/1‑60 (MQ=255) tACTGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGtct > 1:3226970/1‑60 (MQ=255) tACTGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGtct > 1:3772635/1‑60 (MQ=255) | TTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGG > minE/317901‑318032 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |