| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 168,409 | G→C | 7.4% | intergenic (‑78/‑290) | map ← / → rpsB | methionine aminopeptidase/30S ribosomal subunit protein S2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 168,409 | 0 | G | C | 7.4% | 258.1 / 8.8 | 81 | intergenic (‑78/‑290) | map/rpsB | methionine aminopeptidase/30S ribosomal subunit protein S2 |
| Reads supporting (aligned to +/- strand): ref base G (39/36); new base C (4/2); total (43/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01 | |||||||||||
TCCATCAGCGTCGGTGATACCGACAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGC > minE/168340‑168479 | tCCATCAGCGTCGGTGATACCGACAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTg < 1:816934/70‑1 (MQ=255) gCGTCGGTGATACCGACAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCAt < 1:3844444/71‑1 (MQ=255) gCGTCGGTGATACCGACAATATATATGTAAGTGCCGGCAATGGTATCACACCCGGGCAAATTGAGAATCAt < 1:1972023/71‑1 (MQ=255) tCGGTGATACCGACAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCt > 1:2036739/1‑71 (MQ=255) ggTGATACCGACAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTg > 1:1431332/1‑70 (MQ=255) tGATACCGACAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAAt < 1:1984200/71‑1 (MQ=255) tACCGACAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTc > 1:3177109/1‑71 (MQ=255) aCCGACAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCg > 1:4222670/1‑71 (MQ=255) ccGACAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCg < 1:2769455/70‑1 (MQ=255) ccGACAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCg < 1:156460/70‑1 (MQ=255) gACAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGcc > 1:2783615/1‑70 (MQ=255) gACAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGcc > 1:904968/1‑70 (MQ=255) gACAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGcc > 1:4478081/1‑70 (MQ=255) gACAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCa < 1:844703/71‑1 (MQ=255) gACAATATATATGTAAGTGCAGTCAATGGTATCACACCCGGGCAAATTGAGaa > 1:251794/1‑53 (MQ=255) aCAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCaa < 1:3465452/71‑1 (MQ=255) aCAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCa > 1:779564/1‑70 (MQ=255) aaTATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAAc < 1:894310/71‑1 (MQ=255) atatatatGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAAt < 1:3227966/50‑1 (MQ=255) tatatatGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACg < 1:896601/70‑1 (MQ=255) tatatatGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGt > 1:2871758/1‑71 (MQ=255) tatatatGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGt > 1:720679/1‑71 (MQ=255) tatatGTAAGTGCCGTCAATGGTATCACAGCCGGGCAAATTGAGAATCATTCTGAATTTCGCCaa < 1:1739592/65‑1 (MQ=255) tGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTga > 1:4444075/1‑39 (MQ=255) tGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTga > 1:326538/1‑39 (MQ=255) tGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTga > 1:4218977/1‑39 (MQ=255) tGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGaa > 1:2894666/1‑42 (MQ=255) tGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAAtt < 1:579000/54‑1 (MQ=255) tGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAAtt < 1:134023/54‑1 (MQ=255) gTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAAtt < 1:3399784/53‑1 (MQ=255) gTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAgg < 1:1592850/71‑1 (MQ=255) gCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGtt > 1:1415737/1‑71 (MQ=255) ccGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGttt < 1:2095226/71‑1 (MQ=255) ccGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGttt < 1:4193212/71‑1 (MQ=255) ccGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGttt < 1:1051786/71‑1 (MQ=255) ccGTCAATGGTATCACACCCGGGCAAATTCAGAATGATTCTCaa < 1:4227387/44‑1 (MQ=25) ccGTCAATGGTATCACACCCGGGCAAATTCAGAATGATTCTCaa < 1:4110454/44‑1 (MQ=25) gTCAATGGTATCACACCCGGGCAAATTCAGAATTATTCTCAAtt > 1:4169795/1‑44 (MQ=25) gTCAATGGTATCACACCCGGGCAAATTCAGAATGATTCTCAAtt > 1:3001849/1‑44 (MQ=21) gTCAATGGTATCACACCCGGGCAAATTCAGAATGATTCTCAAtt > 1:2406428/1‑44 (MQ=21) gTCAATGGTATCACACCCGGGCAAATTCAGAATGATTCTCAAtt > 1:1898710/1‑44 (MQ=21) cAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCta > 1:1510324/1‑71 (MQ=255) aaTGGTATCACACCCGGGCAAATTGAGAATCATTCTGAAtt > 1:508737/1‑41 (MQ=255) aaTGGTATCACACCCGGGCAAATTGAGAATCATTCTGAAtt > 1:2821829/1‑41 (MQ=255) aaTGGTATCACACCCGGGCAAATTGAGAATCATTCTGAAtt > 1:4674065/1‑41 (MQ=255) ttGGTATCACACCCGGGCAAATTGAGAATCATTCTGAAtt < 1:1798214/39‑1 (MQ=255) aTGGTATCACACCCGGGCAAATTGAGAATCATTCTGAAtt < 1:4263996/40‑1 (MQ=255) aTGGTATCACACCCGGGCAAATTGAGAATCATTCTGAAtt < 1:1064877/40‑1 (MQ=255) aTGGTATCACACCCGGGCAAATTGAGAATCATTCTGAAtt < 1:2970492/40‑1 (MQ=255) aTGGTATCACACCCGGGCAAATTGAGAATCATTCTGAAtt < 1:1911466/40‑1 (MQ=255) aTGGTATCACACCCGGGCAAATTGAGAATCATTCTGAAtt < 1:2599008/40‑1 (MQ=255) ggTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTataa > 1:180078/1‑70 (MQ=255) gTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTataata < 1:3720988/71‑1 (MQ=255) gTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTataata < 1:1367233/71‑1 (MQ=255) tCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGt < 1:4003258/45‑1 (MQ=255) tCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGt < 1:4520036/45‑1 (MQ=255) tCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGt < 1:1529923/45‑1 (MQ=255) tCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTataat < 1:1812842/67‑1 (MQ=255) tCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGa > 1:4762029/1‑70 (MQ=255) acacCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGtttt > 1:41808/1‑58 (MQ=255) acacCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGtttt > 1:2319643/1‑58 (MQ=255) acacCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGtttt > 1:2899849/1‑58 (MQ=255) acacCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGaaaa > 1:4226054/1‑71 (MQ=255) cacCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGa < 1:3354600/50‑1 (MQ=255) cacCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGa < 1:998523/50‑1 (MQ=255) cccGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAg > 1:4088802/1‑50 (MQ=255) cccGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAg > 1:1106982/1‑50 (MQ=255) cccGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAg > 1:663302/1‑50 (MQ=255) cccGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTc > 1:4088360/1‑71 (MQ=255) cccGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTc > 1:151807/1‑71 (MQ=255) cccGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGACACTGAAGGTTTTCTATAATAGAAAATTc > 1:1265642/1‑71 (MQ=255) ccGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAgg > 1:1463696/1‑50 (MQ=255) ccGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTc > 1:2634485/1‑70 (MQ=255) ccGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTc < 1:1765057/70‑1 (MQ=255) ccGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTc < 1:2746858/70‑1 (MQ=255) cGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGa > 1:704739/1‑71 (MQ=255) gggCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGAc < 1:1609067/71‑1 (MQ=255) ggCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGa < 1:168072/44‑1 (MQ=255) cAAATTGGGAATCATTCTGAATTTCGCCAAACGTGc > 1:287417/1‑36 (MQ=255) aaaTTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCt > 1:3995589/1‑71 (MQ=255) aaTTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCt > 1:4383859/1‑70 (MQ=255) aTTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGa > 1:430261/1‑64 (MQ=255) aTTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGa > 1:1580488/1‑71 (MQ=255) tGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATg > 1:717955/1‑71 (MQ=255) tGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATg > 1:877752/1‑71 (MQ=255) gagaATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATg < 1:1090251/70‑1 (MQ=255) gagaATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGc > 1:3903866/1‑71 (MQ=255) gagaATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGc > 1:1770857/1‑71 (MQ=255) gagaATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGc > 1:1765332/1‑71 (MQ=255) | TCCATCAGCGTCGGTGATACCGACAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGC > minE/168340‑168479 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |