| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,184,264 | G→C | 8.7% | V1514L (GTG→CTG) | gltB → | glutamate synthase, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,184,264 | 0 | G | C | 8.7% | 244.7 / 10.5 | 80 | V1514L (GTG→CTG) | gltB | glutamate synthase, large subunit |
| Reads supporting (aligned to +/- strand): ref base G (35/38); new base C (2/5); total (37/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01 | |||||||||||
GTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTG > minE/2184195‑2184333 | gTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGt > 1:1278199/1‑71 (MQ=255) ttAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTg < 1:1791544/71‑1 (MQ=255) ttAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCCTg > 1:1370360/1‑71 (MQ=255) ttAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCCTg > 1:4504886/1‑71 (MQ=255) aaaCCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTg > 1:2505207/1‑69 (MQ=255) aaaCCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTg > 1:1330327/1‑69 (MQ=255) aaaCCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGc < 1:1902234/70‑1 (MQ=255) aCCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAgg > 1:3203267/1‑71 (MQ=255) ccGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCCTGCACgc < 1:1846075/71‑1 (MQ=255) ccGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCCTGCACgc < 1:1445086/71‑1 (MQ=255) ccGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCCTGCACgc < 1:3450594/71‑1 (MQ=255) cGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCCTGCACgc < 1:2151627/70‑1 (MQ=255) cGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCCTGCACgc < 1:1696413/70‑1 (MQ=255) aaGTCCAGTGATGTAAAAGCACTGCTGAGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGgcg > 1:3157460/1‑69 (MQ=255) aGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAg > 1:1322400/1‑71 (MQ=255) aGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAg > 1:4528427/1‑71 (MQ=255) gTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGt < 1:4206141/71‑1 (MQ=255) gTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGt < 1:4025719/71‑1 (MQ=255) gTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGt < 1:4315274/71‑1 (MQ=255) gTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGt < 1:1379512/71‑1 (MQ=255) cAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGt > 1:3601727/1‑68 (MQ=255) cAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTaa < 1:886839/70‑1 (MQ=255) aGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAgg > 1:3934487/1‑71 (MQ=255) gTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCa > 1:3463785/1‑58 (MQ=255) gTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCa > 1:1339372/1‑58 (MQ=255) gTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCa > 1:1130888/1‑58 (MQ=255) gTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCa > 1:2461893/1‑58 (MQ=255) gTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCa > 1:2280561/1‑58 (MQ=255) tGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTg < 1:1269762/55‑1 (MQ=255) tGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTg < 1:2713663/55‑1 (MQ=255) tGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTg < 1:2516960/55‑1 (MQ=255) tGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAg < 1:278486/64‑1 (MQ=255) aTGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCa > 1:3135228/1‑61 (MQ=255) aTGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCa > 1:304471/1‑61 (MQ=255) aTGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCa > 1:2147531/1‑61 (MQ=255) aTGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAgggg > 1:3984489/1‑69 (MQ=255) aTGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAgggg > 1:1000934/1‑69 (MQ=255) aTGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGt < 1:3590621/70‑1 (MQ=255) aTGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGt < 1:2522257/70‑1 (MQ=255) aTGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGt < 1:851476/70‑1 (MQ=255) tGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGcgcg > 1:2191079/1‑50 (MQ=255) tGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGcgcg > 1:3868514/1‑50 (MQ=255) tGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGcgcg > 1:3814286/1‑50 (MQ=255) tGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGcgcg > 1:3581571/1‑50 (MQ=255) gTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTaa > 1:3374301/1‑63 (MQ=255) gTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTaa > 1:1897343/1‑63 (MQ=255) gTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTaa > 1:3010323/1‑63 (MQ=255) tAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGc > 1:1102846/1‑51 (MQ=255) tAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGc > 1:178585/1‑70 (MQ=255) tAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGc < 1:1711871/70‑1 (MQ=255) tAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGc > 1:3169019/1‑70 (MQ=255) aaaaGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGcaa > 1:634286/1‑71 (MQ=255) aGCACTGCTGGGTAACCGTAGTCGTAGCGCAGCTGAGTTGAGCGTGCAGGCGCAGTaa > 1:1026323/1‑58 (MQ=255) tgctgGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAgg < 1:593336/55‑1 (MQ=255) ctgGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCa < 1:4394387/71‑1 (MQ=255) ctgGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCa < 1:1617742/71‑1 (MQ=255) ggTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAg < 1:2076735/69‑1 (MQ=255) tCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGt < 1:1000618/44‑1 (MQ=255) tCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTa < 1:4426341/45‑1 (MQ=255) tCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTa < 1:4356533/45‑1 (MQ=255) gTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTAt > 1:4592943/1‑71 (MQ=255) gTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTAt > 1:1331682/1‑71 (MQ=255) tAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAgg < 1:2655719/42‑1 (MQ=255) tAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAgg < 1:2119265/42‑1 (MQ=255) tAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAgg < 1:3721327/42‑1 (MQ=255) gTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCaa > 1:2103935/1‑71 (MQ=255) gTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCa < 1:1636805/70‑1 (MQ=255) gTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCa < 1:542104/70‑1 (MQ=255) tCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGt < 1:225982/42‑1 (MQ=255) tCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGt < 1:1681310/42‑1 (MQ=255) gTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAAttt < 1:3214189/71‑1 (MQ=255) tAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATg < 1:3867387/59‑1 (MQ=255) tAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATg < 1:2153469/59‑1 (MQ=255) cgcAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCg < 1:4223801/71‑1 (MQ=255) aGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGAcc > 1:1704260/1‑71 (MQ=255) gCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGcaac < 1:1169761/38‑1 (MQ=255) gCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGa < 1:3685616/68‑1 (MQ=255) gCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGa < 1:3994707/68‑1 (MQ=255) tGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGcaacaa > 1:1268425/1‑38 (MQ=255) tGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGcaacaa > 1:3120725/1‑38 (MQ=255) tGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAgcgc > 1:4013150/1‑71 (MQ=255) gcgcGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAgcgc < 1:1642448/70‑1 (MQ=255) gcgcGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAgcgc < 1:2175729/70‑1 (MQ=255) gcgcGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGcgcg > 1:4581690/1‑71 (MQ=255) gcgTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGa > 1:4201174/1‑58 (MQ=255) cgTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTg < 1:1089375/71‑1 (MQ=255) cgTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTg < 1:3568843/71‑1 (MQ=255) cgTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTg < 1:3505073/71‑1 (MQ=255) cgTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTg < 1:254752/71‑1 (MQ=255) cgTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTg < 1:985507/71‑1 (MQ=255) | GTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCTGAGTTGCGCGTGCAGGCGCAGTAAGGGGTAGCAACAATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTG > minE/2184195‑2184333 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |