| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,281,121 | A→G | 12.6% | F67S (TTT→TCT) | fabR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,281,121 | 0 | A | G | 12.6% | 309.2 / 31.1 | 111 | F67S (TTT→TCT) | fabR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base A (58/39); new base G (3/11); total (61/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.23e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
Only 100 of 124 total aligned reads displayed.
TAGCATTAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTT > minE/2281054‑2281187 | tAGCATTAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATaaa > 1:362282/1‑68 (MQ=255) ttAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGt < 1:1883399/70‑1 (MQ=255) ttAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGt < 1:1944587/70‑1 (MQ=255) ttAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGt < 1:75722/70‑1 (MQ=255) ttAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTg > 1:1162278/1‑71 (MQ=255) tAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTgg > 1:4056472/1‑71 (MQ=255) aaaCCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAaga > 1:2678681/1‑65 (MQ=255) aaaCCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAaga > 1:3605844/1‑65 (MQ=255) aaaCCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAaga > 1:58302/1‑65 (MQ=255) aaaCCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTgg > 1:2500561/1‑70 (MQ=255) aaCCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTggg < 1:2760253/70‑1 (MQ=255) ccGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGc < 1:2446103/71‑1 (MQ=255) ccGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGc < 1:1428683/71‑1 (MQ=255) ccGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGc < 1:2111345/71‑1 (MQ=255) gCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCa > 1:3690632/1‑70 (MQ=255) gCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCa > 1:2712200/1‑70 (MQ=255) ctctCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAAt > 1:3618362/1‑71 (MQ=255) ctctCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAaga > 1:834149/1‑59 (MQ=255) ctctCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAaga > 1:814609/1‑59 (MQ=255) ctctCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAaga > 1:132026/1‑59 (MQ=255) ctctCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAaga > 1:1332810/1‑59 (MQ=255) tctcATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAAt < 1:1719115/70‑1 (MQ=255) tcatcaACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATaaa < 1:578627/52‑1 (MQ=255) atcaACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCg > 1:2189916/1‑71 (MQ=255) tcaACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATaaaaa < 1:2650614/51‑1 (MQ=255) caACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCt > 1:4444369/1‑71 (MQ=255) aaCCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTg > 1:2685463/1‑55 (MQ=255) aaCCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCtt > 1:607824/1‑71 (MQ=255) aCCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCtt > 1:1276232/1‑70 (MQ=255) aCCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTc < 1:3045094/71‑1 (MQ=255) cATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCAc < 1:4546479/71‑1 (MQ=255) cATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCAc < 1:4541158/71‑1 (MQ=255) cATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCAc < 1:4642730/71‑1 (MQ=255) cATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCAc < 1:2226269/71‑1 (MQ=255) aTGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGa > 1:1124368/1‑54 (MQ=255) aTGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGa < 1:1127329/54‑1 (MQ=255) aTGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGa < 1:3437122/54‑1 (MQ=255) aTGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGa < 1:4089359/54‑1 (MQ=255) aTGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCAcg > 1:3628391/1‑71 (MQ=255) tGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCTGATaaa < 1:607806/42‑1 (MQ=255) ggTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGc > 1:3098197/1‑54 (MQ=255) ggTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGc > 1:3010820/1‑54 (MQ=255) gTCAGACGCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTc < 1:2716995/65‑1 (MQ=255) gTCAGACCCATTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTc < 1:1043808/65‑1 (MQ=255) gTCAGACCCATTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTc < 1:1049450/65‑1 (MQ=255) gTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATaaaaa > 1:2972096/1‑42 (MQ=255) gTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATaaaaa > 1:415356/1‑42 (MQ=255) gTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATaaaaa > 1:259987/1‑42 (MQ=255) gTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATaaaaa > 1:2006761/1‑42 (MQ=255) gTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATaaaaa > 1:2635020/1‑42 (MQ=255) gTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTc < 1:226677/65‑1 (MQ=255) cAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCAc > 1:3773836/1‑65 (MQ=255) cAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCAc > 1:3124256/1‑65 (MQ=255) gACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACt > 1:44703/1‑71 (MQ=255) gACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACt > 1:1033887/1‑71 (MQ=255) gACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACt > 1:2701001/1‑71 (MQ=255) aCCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAag < 1:4160218/38‑1 (MQ=255) cccAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTc < 1:632778/71‑1 (MQ=255) cccAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGGGGGAGCAATGCCCGCTTCACGCGCCACtt < 1:4258837/70‑1 (MQ=255) gTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACg > 1:351109/1‑70 (MQ=255) gTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGc < 1:1202459/71‑1 (MQ=255) tCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCaa > 1:2522040/1‑43 (MQ=255) tCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCa > 1:1705212/1‑70 (MQ=255) tCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCa > 1:586299/1‑70 (MQ=255) tCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCa > 1:4098170/1‑70 (MQ=255) tCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCa > 1:2557487/1‑70 (MQ=255) cGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCaa > 1:4494562/1‑42 (MQ=255) tCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACt < 1:554570/71‑1 (MQ=255) tCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACt < 1:260422/71‑1 (MQ=255) cTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCtt < 1:2113926/48‑1 (MQ=255) cTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCaaa > 1:2240063/1‑68 (MQ=255) cTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACt < 1:4700569/70‑1 (MQ=255) tACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCaaa > 1:3211474/1‑67 (MQ=255) tACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCaaa > 1:1431776/1‑67 (MQ=255) cGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTc < 1:4417231/68‑1 (MQ=255) cgcgGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTg > 1:2660571/1‑71 (MQ=255) cgcgGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTg > 1:4227022/1‑71 (MQ=255) cgcgGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTg > 1:349384/1‑71 (MQ=255) gAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGa < 1:3731383/71‑1 (MQ=255) gAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGa < 1:3645338/71‑1 (MQ=255) aaaTGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGc > 1:3755620/1‑64 (MQ=255) aaaTGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCg > 1:4223610/1‑69 (MQ=255) aaaTGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCg > 1:472628/1‑69 (MQ=255) aaaTGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGaa < 1:736714/71‑1 (MQ=255) aaTGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAg > 1:2853216/1‑71 (MQ=255) aaTGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAg > 1:3450735/1‑71 (MQ=255) aTGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCGCGCAAACTCAGGCTGGCGAAGc > 1:3000530/1‑71 (MQ=255) ccGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCa < 1:2087841/50‑1 (MQ=255) ccGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCa > 1:1754031/1‑56 (MQ=255) ccGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCa > 1:216548/1‑56 (MQ=255) ccGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCa > 1:3744219/1‑56 (MQ=255) ccGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTg < 1:560907/70‑1 (MQ=255) ccGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTg < 1:1592815/70‑1 (MQ=255) ccGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTg < 1:2536118/70‑1 (MQ=255) ccGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTg < 1:3749542/70‑1 (MQ=255) ccGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTg < 1:2941596/70‑1 (MQ=255) cGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCg < 1:134704/71‑1 (MQ=255) cGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCg < 1:781316/71‑1 (MQ=255) gATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGt > 1:1997464/1‑71 (MQ=255) aTAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGtt < 1:3043730/71‑1 (MQ=255) | TAGCATTAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGTT > minE/2281054‑2281187 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |