Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,335,293 T→C 13.2% I914I (ATT→ATC fdoG → formate dehydrogenase‑O, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,335,2930TC13.2% 373.1 / 42.5 136I914I (ATT→ATCfdoGformate dehydrogenase‑O, large subunit
Reads supporting (aligned to +/- strand):  ref base T (64/54);  new base C (12/6);  total (76/60)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01

Only 100 of 148 total aligned reads displayed.

TCGGAACCACCTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGCCCAGGGCGAT  >  minE/2335226‑2335362
                                                                   |                                                                     
tCGGAACCACCTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTTAACGCGATTTTg                                                                    <  1:3179855/71‑1 (MQ=255)
      ccaccTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGc                                                                >  1:4198840/1‑69 (MQ=255)
      ccaccTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGc                                                                >  1:1743360/1‑69 (MQ=255)
       caccTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGc                                                                <  1:246467/68‑1 (MQ=255)
       caccTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGc                                                                <  1:2595796/68‑1 (MQ=255)
       caccTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGc                                                                <  1:4288774/68‑1 (MQ=255)
         ccTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCagag                                                           >  1:4159454/1‑71 (MQ=255)
         ccTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCagag                                                           >  1:457268/1‑71 (MQ=255)
         ccTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATCTTGCAGCCagag                                                           >  1:1027288/1‑71 (MQ=255)
         ccTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATCTTGCAGCCagag                                                           >  1:1915378/1‑71 (MQ=255)
         ccTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATCTTGCAGCCagag                                                           >  1:1768497/1‑71 (MQ=255)
         ccTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATCTTGCAGCCagag                                                           >  1:506072/1‑71 (MQ=255)
         ccTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATCTTGCAGCCagag                                                           >  1:582247/1‑71 (MQ=255)
           tATCGTCTGACCGAGCACTTCCACTACTGCACCAAACACGCGCTGTTGAACGCGAtttt                                                                     >  1:2018028/1‑59 (MQ=255)
             tCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATCTTGCAGCCAGAGCa                                                         <  1:834895/69‑1 (MQ=255)
             tCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATCTTGCAGCCAGAGCa                                                         <  1:4365338/69‑1 (MQ=255)
             tCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATCTTGCAGCCAGAGCa                                                         <  1:3541154/69‑1 (MQ=255)
             tCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATCTTGCAGCCAGAGCa                                                         <  1:2136058/69‑1 (MQ=255)
               gTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGttt                                                     >  1:437292/1‑71 (MQ=255)
               gTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGtt                                                      <  1:4034348/70‑1 (MQ=255)
                tCTGACCGAGGACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTtg                                                    <  1:4182885/71‑1 (MQ=255)
                tCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTtg                                                    <  1:2391688/71‑1 (MQ=255)
                tCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGCTGAACGCGATTTTGCAGCCAGAGCAGTTtg                                                    <  1:2419174/71‑1 (MQ=255)
                  tGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTtgtg                                                  >  1:1981731/1‑71 (MQ=255)
                  tGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTtgtg                                                  >  1:1725101/1‑71 (MQ=255)
                     ccGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCag                                                             >  1:4691331/1‑57 (MQ=255)
                     ccGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCag                                                             >  1:182859/1‑57 (MQ=255)
                     ccGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCag                                                             >  1:2510198/1‑57 (MQ=255)
                     ccGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGAAGCCag                                                             >  1:4486509/1‑57 (MQ=255)
                       gAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGaaa                                              >  1:2161445/1‑70 (MQ=255)
                           aCTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCggg                                         <  1:3902130/71‑1 (MQ=255)
                            cTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGc                                                                <  1:808064/47‑1 (MQ=255)
                               ccACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGt                                                       >  1:1311779/1‑53 (MQ=255)
                                cACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGTGGAGTc                                    <  1:607339/71‑1 (MQ=255)
                                cACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTGTGGAAATCGGGGAGTc                                    <  1:3662721/70‑1 (MQ=255)
                                cACTACTGGACCAAACACGCGCTGTTGAACGCGATCTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTc                                    >  1:1202043/1‑71 (MQ=255)
                                  ctactGGACCAAACACGCGCTGTTGAACGCGATTTTTCAGCCAGAGCAGTTTGTGGAAATCggg                                         <  1:269965/64‑1 (MQ=255)
                                  ctactGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCa                                                              >  1:1328048/1‑43 (MQ=255)
                                  ctactGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCggg                                         <  1:71880/64‑1 (MQ=255)
                                  ctactGGACCAAACACGCG‑‑GTTGAACGCGATTTTGCAGCCa                                                              >  1:97676/1‑41 (MQ=255)
                                   tactGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCt                                 >  1:954523/1‑71 (MQ=255)
                                     ctGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTtgtg                                                  >  1:2085440/1‑52 (MQ=255)
                                     ctGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTtgtg                                                  >  1:661951/1‑52 (MQ=255)
                                     ctGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTtgtg                                                  >  1:3325178/1‑52 (MQ=255)
                                     ctGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATc                                            <  1:1852647/58‑1 (MQ=255)
                                     ctGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCt                                 <  1:546429/69‑1 (MQ=255)
                                     ctGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCt                                 <  1:2104170/69‑1 (MQ=255)
                                          ccAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGaaa                                              >  1:3111730/1‑51 (MQ=255)
                                          ccAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGaaa                                              >  1:821510/1‑51 (MQ=255)
                                           cAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCt                                 <  1:1372381/63‑1 (MQ=255)
                                           cAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCt                                 <  1:3795760/63‑1 (MQ=255)
                                           cAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCt                                 <  1:1241586/63‑1 (MQ=255)
                                             aaCACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGtt                                                      <  1:3808320/40‑1 (MQ=255)
                                             aaCACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCggg                                         >  1:2694130/1‑53 (MQ=255)
                                             aaCACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTgg                               >  1:3232960/1‑63 (MQ=255)
                                             aaCACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTgg                               >  1:195552/1‑63 (MQ=255)
                                             aaCACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTgg                               >  1:3908437/1‑63 (MQ=255)
                                             aaCACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTg                                >  1:871783/1‑62 (MQ=255)
                                             aaCACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTg                                >  1:1077371/1‑62 (MQ=255)
                                              acacGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTtg                                                    >  1:1923439/1‑41 (MQ=255)
                                              acacGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTtg                                                    >  1:3019389/1‑41 (MQ=255)
                                              acacGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAAc                      <  1:3375814/71‑1 (MQ=255)
                                                acGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCggg                                         <  1:4276792/50‑1 (MQ=255)
                                                acGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACtt                    >  1:671144/1‑71 (MQ=255)
                                                 cgcgCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTg                   <  1:1216117/71‑1 (MQ=255)
                                                  gcgcTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAg                                      >  1:4074140/1‑51 (MQ=255)
                                                  gcgcTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTgg                  <  1:3052240/71‑1 (MQ=255)
                                                  gcgcTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTgg                  <  1:4128417/71‑1 (MQ=255)
                                                  gcgcTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTgg                  <  1:1937107/71‑1 (MQ=255)
                                                  gcgcTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTgg                  <  1:1493185/71‑1 (MQ=255)
                                                  gcgcTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTgg                  <  1:1767508/71‑1 (MQ=255)
                                                      tGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAAt                                             <  1:2869146/40‑1 (MQ=255)
                                                      tGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAAt                                             <  1:2025097/40‑1 (MQ=255)
                                                      tGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAAt                                             <  1:341402/40‑1 (MQ=255)
                                                       gTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCa                >  1:3642211/1‑68 (MQ=255)
                                                       gTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTg             >  1:1018812/1‑71 (MQ=255)
                                                        ttGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGc            >  1:1320029/1‑71 (MQ=255)
                                                        ttGAACGCGATCTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGc            >  1:2381223/1‑71 (MQ=255)
                                                        ttGAACGCGATCTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGc            >  1:854837/1‑71 (MQ=255)
                                                         tGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGcc           >  1:1407908/1‑71 (MQ=255)
                                                          gAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGc                 >  1:4479106/1‑64 (MQ=255)
                                                          gAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGc                 >  1:2433442/1‑64 (MQ=255)
                                                           aaCGCGATTTTGCAGCCAGAGCAGTTTGTGGAAAt                                             >  1:3297219/1‑35 (MQ=255)
                                                           aaCGCGATTTTGCAGCCAGAGCAGTTTGTGGAAAt                                             >  1:2719861/1‑35 (MQ=255)
                                                             cgcgATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGCCCAgg       >  1:1541639/1‑71 (MQ=255)
                                                             cgcgATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGCCCAgg       >  1:3492642/1‑71 (MQ=255)
                                                             cgcgATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGCCCAgg       >  1:2235263/1‑71 (MQ=255)
                                                             cgcgATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGCCCAgg       >  1:4700864/1‑71 (MQ=255)
                                                              gcgATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGc                 <  1:356506/60‑1 (MQ=255)
                                                              gcgATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGc                 <  1:3477433/60‑1 (MQ=255)
                                                               cgATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGCCCAGGGc     <  1:836653/71‑1 (MQ=255)
                                                                gATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCAt               >  1:4498354/1‑60 (MQ=255)
                                                                 aTTTTGCAGCCAGAGCAGTTTGTGTAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGCCCAg        >  1:1536963/1‑66 (MQ=255)
                                                                  ttttGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTgg                               >  1:2589328/1‑42 (MQ=255)
                                                                  ttttGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTg                   >  1:24238/1‑54 (MQ=255)
                                                                  ttttGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGGATTGCCCAg        >  1:405256/1‑65 (MQ=255)
                                                                  ttttGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGccag         >  1:44103/1‑62 (MQ=255)
                                                                  ttttGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGCCCAg        >  1:711435/1‑65 (MQ=255)
                                                                  ttttGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGCCCAGGGCTAt  <  1:4212926/71‑1 (MQ=255)
                                                                  ttttGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGCCCAGGGCGa   >  1:1470245/1‑70 (MQ=255)
                                                                   |                                                                     
TCGGAACCACCTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGCCCAGGGCGAT  >  minE/2335226‑2335362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: