| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,335,293 | T→C | 13.2% | I914I (ATT→ATC) | fdoG → | formate dehydrogenase‑O, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,335,293 | 0 | T | C | 13.2% | 373.1 / 42.5 | 136 | I914I (ATT→ATC) | fdoG | formate dehydrogenase‑O, large subunit |
| Reads supporting (aligned to +/- strand): ref base T (64/54); new base C (12/6); total (76/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01 | |||||||||||
Only 100 of 148 total aligned reads displayed.
TCGGAACCACCTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGCCCAGGGCGAT > minE/2335226‑2335362 | tCGGAACCACCTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTTAACGCGATTTTg < 1:3179855/71‑1 (MQ=255) ccaccTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGc > 1:4198840/1‑69 (MQ=255) ccaccTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGc > 1:1743360/1‑69 (MQ=255) caccTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGc < 1:246467/68‑1 (MQ=255) caccTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGc < 1:2595796/68‑1 (MQ=255) caccTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGc < 1:4288774/68‑1 (MQ=255) ccTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCagag > 1:4159454/1‑71 (MQ=255) ccTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCagag > 1:457268/1‑71 (MQ=255) ccTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATCTTGCAGCCagag > 1:1027288/1‑71 (MQ=255) ccTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATCTTGCAGCCagag > 1:1915378/1‑71 (MQ=255) ccTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATCTTGCAGCCagag > 1:1768497/1‑71 (MQ=255) ccTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATCTTGCAGCCagag > 1:506072/1‑71 (MQ=255) ccTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATCTTGCAGCCagag > 1:582247/1‑71 (MQ=255) tATCGTCTGACCGAGCACTTCCACTACTGCACCAAACACGCGCTGTTGAACGCGAtttt > 1:2018028/1‑59 (MQ=255) tCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATCTTGCAGCCAGAGCa < 1:834895/69‑1 (MQ=255) tCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATCTTGCAGCCAGAGCa < 1:4365338/69‑1 (MQ=255) tCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATCTTGCAGCCAGAGCa < 1:3541154/69‑1 (MQ=255) tCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATCTTGCAGCCAGAGCa < 1:2136058/69‑1 (MQ=255) gTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGttt > 1:437292/1‑71 (MQ=255) gTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGtt < 1:4034348/70‑1 (MQ=255) tCTGACCGAGGACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTtg < 1:4182885/71‑1 (MQ=255) tCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTtg < 1:2391688/71‑1 (MQ=255) tCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGCTGAACGCGATTTTGCAGCCAGAGCAGTTtg < 1:2419174/71‑1 (MQ=255) tGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTtgtg > 1:1981731/1‑71 (MQ=255) tGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTtgtg > 1:1725101/1‑71 (MQ=255) ccGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCag > 1:4691331/1‑57 (MQ=255) ccGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCag > 1:182859/1‑57 (MQ=255) ccGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCag > 1:2510198/1‑57 (MQ=255) ccGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGAAGCCag > 1:4486509/1‑57 (MQ=255) gAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGaaa > 1:2161445/1‑70 (MQ=255) aCTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCggg < 1:3902130/71‑1 (MQ=255) cTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGc < 1:808064/47‑1 (MQ=255) ccACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGt > 1:1311779/1‑53 (MQ=255) cACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGTGGAGTc < 1:607339/71‑1 (MQ=255) cACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAG‑TTGTGGAAATCGGGGAGTc < 1:3662721/70‑1 (MQ=255) cACTACTGGACCAAACACGCGCTGTTGAACGCGATCTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTc > 1:1202043/1‑71 (MQ=255) ctactGGACCAAACACGCGCTGTTGAACGCGATTTTTCAGCCAGAGCAGTTTGTGGAAATCggg < 1:269965/64‑1 (MQ=255) ctactGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCa > 1:1328048/1‑43 (MQ=255) ctactGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCggg < 1:71880/64‑1 (MQ=255) ctactGGACCAAACACGCG‑‑GTTGAACGCGATTTTGCAGCCa > 1:97676/1‑41 (MQ=255) tactGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCt > 1:954523/1‑71 (MQ=255) ctGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTtgtg > 1:2085440/1‑52 (MQ=255) ctGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTtgtg > 1:661951/1‑52 (MQ=255) ctGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTtgtg > 1:3325178/1‑52 (MQ=255) ctGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATc < 1:1852647/58‑1 (MQ=255) ctGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCt < 1:546429/69‑1 (MQ=255) ctGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCt < 1:2104170/69‑1 (MQ=255) ccAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGaaa > 1:3111730/1‑51 (MQ=255) ccAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGaaa > 1:821510/1‑51 (MQ=255) cAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCt < 1:1372381/63‑1 (MQ=255) cAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCt < 1:3795760/63‑1 (MQ=255) cAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCt < 1:1241586/63‑1 (MQ=255) aaCACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGtt < 1:3808320/40‑1 (MQ=255) aaCACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCggg > 1:2694130/1‑53 (MQ=255) aaCACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTgg > 1:3232960/1‑63 (MQ=255) aaCACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTgg > 1:195552/1‑63 (MQ=255) aaCACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTgg > 1:3908437/1‑63 (MQ=255) aaCACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTg > 1:871783/1‑62 (MQ=255) aaCACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTg > 1:1077371/1‑62 (MQ=255) acacGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTtg > 1:1923439/1‑41 (MQ=255) acacGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTtg > 1:3019389/1‑41 (MQ=255) acacGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAAc < 1:3375814/71‑1 (MQ=255) acGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCggg < 1:4276792/50‑1 (MQ=255) acGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACtt > 1:671144/1‑71 (MQ=255) cgcgCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTg < 1:1216117/71‑1 (MQ=255) gcgcTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAg > 1:4074140/1‑51 (MQ=255) gcgcTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTgg < 1:3052240/71‑1 (MQ=255) gcgcTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTgg < 1:4128417/71‑1 (MQ=255) gcgcTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTgg < 1:1937107/71‑1 (MQ=255) gcgcTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTgg < 1:1493185/71‑1 (MQ=255) gcgcTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTgg < 1:1767508/71‑1 (MQ=255) tGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAAt < 1:2869146/40‑1 (MQ=255) tGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAAt < 1:2025097/40‑1 (MQ=255) tGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAAt < 1:341402/40‑1 (MQ=255) gTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCa > 1:3642211/1‑68 (MQ=255) gTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTg > 1:1018812/1‑71 (MQ=255) ttGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGc > 1:1320029/1‑71 (MQ=255) ttGAACGCGATCTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGc > 1:2381223/1‑71 (MQ=255) ttGAACGCGATCTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGc > 1:854837/1‑71 (MQ=255) tGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGcc > 1:1407908/1‑71 (MQ=255) gAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGc > 1:4479106/1‑64 (MQ=255) gAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGc > 1:2433442/1‑64 (MQ=255) aaCGCGATTTTGCAGCCAGAGCAGTTTGTGGAAAt > 1:3297219/1‑35 (MQ=255) aaCGCGATTTTGCAGCCAGAGCAGTTTGTGGAAAt > 1:2719861/1‑35 (MQ=255) cgcgATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGCCCAgg > 1:1541639/1‑71 (MQ=255) cgcgATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGCCCAgg > 1:3492642/1‑71 (MQ=255) cgcgATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGCCCAgg > 1:2235263/1‑71 (MQ=255) cgcgATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGCCCAgg > 1:4700864/1‑71 (MQ=255) gcgATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGc < 1:356506/60‑1 (MQ=255) gcgATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGc < 1:3477433/60‑1 (MQ=255) cgATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGCCCAGGGc < 1:836653/71‑1 (MQ=255) gATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCAt > 1:4498354/1‑60 (MQ=255) aTTTTGCAGCCAGAGCAGTTTGTGTAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGCCCAg > 1:1536963/1‑66 (MQ=255) ttttGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTgg > 1:2589328/1‑42 (MQ=255) ttttGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTg > 1:24238/1‑54 (MQ=255) ttttGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGGATTGCCCAg > 1:405256/1‑65 (MQ=255) ttttGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGccag > 1:44103/1‑62 (MQ=255) ttttGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGCCCAg > 1:711435/1‑65 (MQ=255) ttttGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGCCCAGGGCTAt < 1:4212926/71‑1 (MQ=255) ttttGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGCCCAGGGCGa > 1:1470245/1‑70 (MQ=255) | TCGGAACCACCTATCGTCTGACCGAGCACTTCCACTACTGGACCAAACACGCGCTGTTGAACGCGATTTTGCAGCCAGAGCAGTTTGTGGAAATCGGGGAGTCGCTGGCGAATAAACTTGGCATTGCCCAGGGCGAT > minE/2335226‑2335362 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |