Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,464,999 G→A 8.5% E219E (GAG→GAA atpB → F0 sector of membrane‑bound ATP synthase, subunit a

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,464,9990GA 8.5% 333.8 / 15.2 106E219E (GAG→GAAatpBF0 sector of membrane‑bound ATP synthase, subunit a
Reads supporting (aligned to +/- strand):  ref base G (43/54);  new base A (4/5);  total (47/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 109 total aligned reads displayed.

AAGGGGTAAGCCTGCTGTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGC  >  minE/2464929‑2465067
                                                                      |                                                                    
aaGGGGTAAGCCTGCTGTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAg                                                                      >  1:2165477/1‑71 (MQ=255)
     gTAAGCCTGCTGTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAgatgat                                                                 >  1:3444992/1‑71 (MQ=255)
      tAAGCCTGCTGTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGAtt                                                                >  1:3186124/1‑71 (MQ=255)
        aGCCTGCTGTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGAtttt                                                              <  1:2561776/71‑1 (MQ=255)
         gCCTGCTGTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGAttt                                                               >  1:2665084/1‑69 (MQ=255)
         gCCTGCTGTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTc                                                             <  1:4053179/71‑1 (MQ=255)
          ccTGCTGTCCAAACCAGTTTCTCTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCa                                                            <  1:1892437/71‑1 (MQ=255)
            tgctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCAtt                                                          >  1:3229136/1‑71 (MQ=255)
            tgctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCAt                                                           >  1:1011043/1‑70 (MQ=255)
            tgctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCAt                                                           >  1:1971062/1‑70 (MQ=255)
              ctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCAtt                                                          <  1:509070/69‑1 (MQ=255)
              ctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCAtt                                                          <  1:1997682/69‑1 (MQ=255)
              ctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCt                                                        <  1:261660/71‑1 (MQ=255)
              ctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCt                                                        >  1:2360461/1‑71 (MQ=255)
              ctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCt                                                        <  1:2292513/71‑1 (MQ=255)
              ctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCt                                                        >  1:1309480/1‑71 (MQ=255)
              ctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCt                                                        >  1:4256742/1‑71 (MQ=255)
              ctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCGGATTTTCATTCt                                                        <  1:2045384/71‑1 (MQ=255)
              ctgTCCAAACCAGTTTCACTCGGTTAGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCt                                                        >  1:3186632/1‑71 (MQ=255)
                 tCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCt                                                                    <  1:2839085/56‑1 (MQ=255)
                 tCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTg                                                       <  1:3754255/69‑1 (MQ=255)
                 tCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGa                                                      <  1:2036765/70‑1 (MQ=255)
                   cAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAgt                                                                     <  1:4436849/53‑2 (MQ=255)
                   cAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGc                                                                     <  1:2655385/53‑1 (MQ=255)
                   cAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGc                                                                     <  1:2388560/53‑1 (MQ=255)
                   cAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTg                                                       <  1:654281/67‑1 (MQ=255)
                   cAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTg                                                       <  1:4224973/67‑1 (MQ=255)
                   cAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGa                                                      >  1:3273336/1‑68 (MQ=255)
                   cAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGAtt                                                    >  1:159969/1‑70 (MQ=255)
                   cAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTg                                                   <  1:2108013/71‑1 (MQ=255)
                   cAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTg                                                   <  1:2102441/71‑1 (MQ=255)
                   cAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTg                                                   <  1:1979820/71‑1 (MQ=255)
                   cAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGACGGTGAGCTGATTTTCa                                                            >  1:2473610/1‑62 (MQ=255)
                   cAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGACGGTGAGCTGATTTTCATTCTg                                                       <  1:154939/67‑1 (MQ=255)
                    aaaCCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGc                                                  <  1:1104878/71‑1 (MQ=255)
                     aaCCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCt                                                 >  1:1807739/1‑71 (MQ=255)
                       ccAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGAttt                                                               >  1:1422812/1‑55 (MQ=255)
                          gTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCt                                            >  1:2906340/1‑71 (MQ=255)
                            ttCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGt                                          >  1:3826078/1‑71 (MQ=255)
                             tCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTg                                                                   >  1:3159068/1‑45 (MQ=255)
                             tCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTg                                                                   >  1:555292/1‑45 (MQ=255)
                              cACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGtt                                         <  1:4451963/70‑1 (MQ=255)
                              cACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGtt                                         <  1:2248924/70‑1 (MQ=255)
                                 tCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCa                                                            <  1:3909304/48‑1 (MQ=255)
                                 tCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCg                                     >  1:2635796/1‑71 (MQ=255)
                                 tCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCg                                     >  1:514956/1‑71 (MQ=255)
                                   ggTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTc                                                         >  1:985076/1‑49 (MQ=255)
                                   ggTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTc                                                         >  1:3938101/1‑49 (MQ=255)
                                   ggTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTc                                                         >  1:133127/1‑49 (MQ=255)
                                   ggTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGa                                                      >  1:1956442/1‑52 (MQ=255)
                                   ggTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCgtg                                   >  1:4747859/1‑71 (MQ=255)
                                   ggTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCg                                     <  1:2739454/69‑1 (MQ=255)
                                   ggTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCg                                     <  1:4102474/69‑1 (MQ=255)
                                     tttGCGACTGTTCTGTAACATGTATGCCGGTGAGCTGATTTTCa                                                            <  1:1176796/44‑1 (MQ=255)
                                     tttGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTct                                                            <  1:198091/44‑2 (MQ=255)
                                     tttGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCa                                                            <  1:1215752/44‑1 (MQ=255)
                                     tttGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCa                                                            <  1:906678/44‑1 (MQ=255)
                                     cttGCGACTGTTCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCgtggt                                 >  1:74925/2‑71 (MQ=255)
                                     cttGCGACTGTTCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCgtggt                                 >  1:3412248/2‑71 (MQ=255)
                                     cttGCGACTGTTCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCgtggt                                 >  1:2293332/2‑71 (MQ=255)
                                     cttGCGACTGTTCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCgtggt                                 >  1:3859644/2‑71 (MQ=255)
                                       tGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGtggtgg                               >  1:3777176/1‑71 (MQ=255)
                                         cGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTggtggt                              <  1:696204/70‑1 (MQ=255)
                                           aCTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTTGTCTGTTGCCGTGGTGGTcac                           <  1:3958726/71‑1 (MQ=255)
                                           aCTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTcac                           <  1:1789411/71‑1 (MQ=255)
                                           aCTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTcac                           <  1:4201384/71‑1 (MQ=255)
                                           aCTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTcac                           <  1:1115985/71‑1 (MQ=255)
                                               ttCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCGTGGTGGTCACAGt                        <  1:64827/70‑1 (MQ=255)
                                               ttCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCGTGGTGGTCACAGt                        <  1:2488532/70‑1 (MQ=255)
                                               ttCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCGTGGTGGTCACAGt                        <  1:4328085/70‑1 (MQ=255)
                                               ttCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCGTGGTGGTCACAGt                        <  1:2154619/70‑1 (MQ=255)
                                               ttCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGATACCGTGGTGGTCACAGt                        <  1:1632785/70‑1 (MQ=255)
                                                 cGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTgg                      <  1:15451/70‑1 (MQ=255)
                                                  ggTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGAt                    <  1:4606629/71‑1 (MQ=255)
                                                  ggTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGAt                    >  1:16380/1‑71 (MQ=255)
                                                   gTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGa                     <  1:1384777/69‑1 (MQ=255)
                                                     aaCATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAg                         >  1:1712296/1‑63 (MQ=255)
                                                      aCATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGAt                    <  1:743229/67‑1 (MQ=255)
                                                      aCATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGAt                    <  1:2764699/67‑1 (MQ=255)
                                                       cATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTg                >  1:3447607/1‑70 (MQ=255)
                                                       cATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTg                >  1:3078560/1‑70 (MQ=255)
                                                       cATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTg                >  1:256207/1‑70 (MQ=255)
                                                       cATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTg                >  1:3863304/1‑70 (MQ=255)
                                                       cATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGa               <  1:2361874/71‑1 (MQ=255)
                                                       cATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGa               <  1:3911839/71‑1 (MQ=255)
                                                         tGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTgg                      >  1:4203597/1‑62 (MQ=255)
                                                         tGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTgg                      >  1:2334127/1‑62 (MQ=255)
                                                           tATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAAtgt           <  1:1362587/71‑1 (MQ=255)
                                                           tATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAAtgt           >  1:2402960/1‑71 (MQ=255)
                                                             tGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGc         >  1:1662452/1‑71 (MQ=255)
                                                               ccGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGcc        >  1:2085340/1‑70 (MQ=255)
                                                               ccGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCg       >  1:3548770/1‑71 (MQ=255)
                                                               ccGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCg       <  1:1048145/71‑1 (MQ=255)
                                                               ccGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCg       <  1:1042453/71‑1 (MQ=255)
                                                                 ggTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTg     >  1:2321249/1‑71 (MQ=255)
                                                                 ggTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTg     >  1:2799251/1‑71 (MQ=255)
                                                                 ggTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTCAATGTGCCGTg     >  1:3131664/1‑71 (MQ=255)
                                                                 ggTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGGCACAGTGGATCCTGAATGTGCCGTg     <  1:4360089/71‑1 (MQ=255)
                                                                  gTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTg     <  1:3695612/70‑1 (MQ=255)
                                                                     aGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGc  <  1:3216128/70‑1 (MQ=255)
                                                                      |                                                                    
AAGGGGTAAGCCTGCTGTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGC  >  minE/2464929‑2465067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: