Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,465,005 T→C 8.7% I221I (ATT→ATC atpB → F0 sector of membrane‑bound ATP synthase, subunit a

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,465,0050TC 8.7% 302.4 / 16.6 103I221I (ATT→ATCatpBF0 sector of membrane‑bound ATP synthase, subunit a
Reads supporting (aligned to +/- strand):  ref base T (41/53);  new base C (4/5);  total (45/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

Only 100 of 115 total aligned reads displayed.

TAAGCCTGCTGTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTC  >  minE/2464935‑2465074
                                                                      |                                                                     
tAAGCCTGCTGTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGAtt                                                                       >  1:3186124/1‑71 (MQ=255)
  aGCCTGCTGTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGAtttt                                                                     <  1:2561776/71‑1 (MQ=255)
  aGCCTGCTGTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGAttt                                                                      >  1:4052313/1‑70 (MQ=255)
   gCCTGCTGTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTc                                                                    <  1:4053179/71‑1 (MQ=255)
      tgctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCAtt                                                                 >  1:3229136/1‑71 (MQ=255)
      tgctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCAt                                                                  >  1:1011043/1‑70 (MQ=255)
      tgctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCAt                                                                  >  1:1971062/1‑70 (MQ=255)
        ctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCAtt                                                                 <  1:1997682/69‑1 (MQ=255)
        ctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCAtt                                                                 <  1:509070/69‑1 (MQ=255)
        ctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCt                                                               <  1:261660/71‑1 (MQ=255)
        ctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCt                                                               >  1:2360461/1‑71 (MQ=255)
        ctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCt                                                               <  1:2292513/71‑1 (MQ=255)
        ctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCGGATTTTCATTCt                                                               <  1:2045384/71‑1 (MQ=255)
        ctgTCCAAACCAGTTTCACTCGGTTAGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCt                                                               >  1:3186632/1‑71 (MQ=255)
         tgTCCAAACCAGTTTCTCTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCAtt                                                                 <  1:4628252/68‑1 (MQ=255)
           tCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGa                                                             <  1:2036765/70‑1 (MQ=255)
             cAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTg                                                              <  1:4224973/67‑1 (MQ=255)
             cAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTg                                                              <  1:654281/67‑1 (MQ=255)
             cAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGa                                                             >  1:3273336/1‑68 (MQ=255)
             cAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTg                                                          <  1:2108013/71‑1 (MQ=255)
             cAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTg                                                          <  1:1979820/71‑1 (MQ=255)
             cAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTg                                                          <  1:2102441/71‑1 (MQ=255)
             cAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGACGGTGAGCTGATTTTCa                                                                   >  1:2473610/1‑62 (MQ=255)
             cAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGACGGTGAGCTGATTTTCATTCTg                                                              <  1:154939/67‑1 (MQ=255)
              aaaCCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGc                                                         <  1:1104878/71‑1 (MQ=255)
               aaCCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCt                                                        >  1:1807739/1‑71 (MQ=255)
                 ccAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGAttt                                                                      >  1:1422812/1‑55 (MQ=255)
                    gTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCt                                                   >  1:2906340/1‑71 (MQ=255)
                      ttCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGt                                                 >  1:3826078/1‑71 (MQ=255)
                        cACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGtt                                                <  1:4451963/70‑1 (MQ=255)
                           tCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCa                                                                   <  1:3909304/48‑1 (MQ=255)
                           tCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCg                                            >  1:514956/1‑71 (MQ=255)
                           tCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCg                                            >  1:2635796/1‑71 (MQ=255)
                             ggTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTc                                                                >  1:3938101/1‑49 (MQ=255)
                             ggTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTc                                                                >  1:133127/1‑49 (MQ=255)
                             ggTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTc                                                                >  1:985076/1‑49 (MQ=255)
                             ggTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGa                                                             >  1:1956442/1‑52 (MQ=255)
                             ggTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCgtg                                          >  1:4747859/1‑71 (MQ=255)
                             ggTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCg                                            <  1:4102474/69‑1 (MQ=255)
                             ggTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCg                                            <  1:2739454/69‑1 (MQ=255)
                              gTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCgtgg                                         <  1:2549788/71‑1 (MQ=255)
                               tttGCGACTGTTCTGTAACATGTATGCCGGTGAGCTGATTTTCa                                                                   <  1:1176796/44‑1 (MQ=255)
                               tttGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTct                                                                   <  1:198091/44‑2 (MQ=255)
                               tttGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCa                                                                   <  1:1452970/44‑1 (MQ=255)
                               tttGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCa                                                                   <  1:906678/44‑1 (MQ=255)
                               tttGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCa                                                                   <  1:1215752/44‑1 (MQ=255)
                               cttGCGACTGTTCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCgtggt                                        >  1:2293332/2‑71 (MQ=255)
                               cttGCGACTGTTCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCgtggt                                        >  1:3859644/2‑71 (MQ=255)
                               cttGCGACTGTTCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCgtggt                                        >  1:74925/2‑71 (MQ=255)
                                 tGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGtggtgg                                      >  1:3777176/1‑71 (MQ=255)
                                     aCTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTcac                                  <  1:1115985/71‑1 (MQ=255)
                                     aCTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTcac                                  <  1:3469622/71‑1 (MQ=255)
                                     aCTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTcac                                  <  1:4201384/71‑1 (MQ=255)
                                     aCTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTcac                                  <  1:1789411/71‑1 (MQ=255)
                                         ttCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCGTGGTGGTCACAGt                               <  1:2488532/70‑1 (MQ=255)
                                         ttCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCGTGGTGGTCACAGt                               <  1:4328085/70‑1 (MQ=255)
                                         ttCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCGTGGTGGTCACAGt                               <  1:2154619/70‑1 (MQ=255)
                                         ttCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCGTGGTGGTCACAGt                               <  1:64827/70‑1 (MQ=255)
                                         ttCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGATACCGTGGTGGTCACAGt                               <  1:1632785/70‑1 (MQ=255)
                                           cGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTgg                             <  1:15451/70‑1 (MQ=255)
                                            ggTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGAt                           <  1:997892/71‑1 (MQ=255)
                                            ggTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGAt                           >  1:2217735/1‑71 (MQ=255)
                                            ggTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGAt                           >  1:16380/1‑71 (MQ=255)
                                             gTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGa                            <  1:1384777/69‑1 (MQ=255)
                                               aaCATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAg                                >  1:1712296/1‑63 (MQ=255)
                                                aCATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGAt                           <  1:743229/67‑1 (MQ=255)
                                                aCATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGAt                           <  1:2764699/67‑1 (MQ=255)
                                                 cATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTg                       >  1:3447607/1‑70 (MQ=255)
                                                 cATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTg                       >  1:256207/1‑70 (MQ=255)
                                                 cATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTg                       >  1:3078560/1‑70 (MQ=255)
                                                 cATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTg                       >  1:3863304/1‑70 (MQ=255)
                                                 cATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGa                      <  1:3911839/71‑1 (MQ=255)
                                                 cATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGa                      <  1:2361874/71‑1 (MQ=255)
                                                 cATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGa                      <  1:1962365/71‑1 (MQ=255)
                                                   tGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTgg                             >  1:4203597/1‑62 (MQ=255)
                                                   tGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTgg                             >  1:2334127/1‑62 (MQ=255)
                                                     tATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAAtgt                  <  1:1362587/71‑1 (MQ=255)
                                                       tGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGc                >  1:1662452/1‑71 (MQ=255)
                                                         ccGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGcc               >  1:2085340/1‑70 (MQ=255)
                                                         ccGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCg              <  1:1048145/71‑1 (MQ=255)
                                                         ccGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCg              >  1:3548770/1‑71 (MQ=255)
                                                         ccGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCg              <  1:1042453/71‑1 (MQ=255)
                                                           ggTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTg            >  1:2321249/1‑71 (MQ=255)
                                                           ggTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTg            >  1:2799251/1‑71 (MQ=255)
                                                           ggTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGGCACAGTGGATCCTGAATGTGCCGTg            <  1:4360089/71‑1 (MQ=255)
                                                            gTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTg            <  1:3695612/70‑1 (MQ=255)
                                                               aGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGc         <  1:3216128/70‑1 (MQ=255)
                                                                 cTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCAt      <  1:4398708/71‑1 (MQ=255)
                                                                 cTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCAt      <  1:4393422/71‑1 (MQ=255)
                                                                 cTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCAt      <  1:520638/71‑1 (MQ=255)
                                                                  tGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCAtt     >  1:4419638/1‑71 (MQ=255)
                                                                   gATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCAttt    >  1:972411/1‑71 (MQ=255)
                                                                   gATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCAttt    >  1:3552012/1‑71 (MQ=255)
                                                                     ttttTATTCTGATTGCTGGTCTGTTGCCGTGGTGGTcaca                                 >  1:3003804/1‑40 (MQ=255)
                                                                      tttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTggg          >  1:3011947/1‑62 (MQ=255)
                                                                      tttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTggg          >  1:3163585/1‑62 (MQ=255)
                                                                      tttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCAtttt   >  1:3544829/1‑69 (MQ=255)
                                                                      tttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTc  >  1:1905292/1‑70 (MQ=255)
                                                                      tttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTc  >  1:446305/1‑70 (MQ=255)
                                                                      tttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTc  >  1:3592156/1‑70 (MQ=255)
                                                                      |                                                                     
TAAGCCTGCTGTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTC  >  minE/2464935‑2465074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: