Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,465,011 T→C 8.5% I223I (ATT→ATC atpB → F0 sector of membrane‑bound ATP synthase, subunit a

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,465,0110TC 8.5% 313.9 / 15.6 105I223I (ATT→ATCatpBF0 sector of membrane‑bound ATP synthase, subunit a
Reads supporting (aligned to +/- strand):  ref base T (52/44);  new base C (4/5);  total (56/49)
Fisher's exact test for biased strand distribution p-value = 7.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

TGCTGTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTCCACA  >  minE/2464941‑2465078
                                                                      |                                                                   
tgctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCAtt                                                                     >  1:3229136/1‑71 (MQ=255)
  ctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCAtt                                                                     <  1:1997682/69‑1 (MQ=255)
  ctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCAtt                                                                     <  1:509070/69‑1 (MQ=255)
  ctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCt                                                                   >  1:4256742/1‑71 (MQ=255)
  ctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCt                                                                   >  1:1309480/1‑71 (MQ=255)
  ctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCt                                                                   <  1:2292513/71‑1 (MQ=255)
  ctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCt                                                                   >  1:2360461/1‑71 (MQ=255)
  ctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCt                                                                   <  1:261660/71‑1 (MQ=255)
  ctgTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCGGATTTTCATTCt                                                                   <  1:2045384/71‑1 (MQ=255)
  ctgTCCAAACCAGTTTCACTCGGTTAGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCt                                                                   >  1:3186632/1‑71 (MQ=255)
   tgTCCAAACCAGTTTCTCTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCAtt                                                                     <  1:4628252/68‑1 (MQ=255)
     tCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTg                                                                  <  1:3754255/69‑1 (MQ=255)
     tCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGa                                                                 <  1:2036765/70‑1 (MQ=255)
       cAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTg                                                                  <  1:654281/67‑1 (MQ=255)
       cAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTg                                                                  <  1:4224973/67‑1 (MQ=255)
       cAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGa                                                                 >  1:3273336/1‑68 (MQ=255)
       cAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGAtt                                                               >  1:159969/1‑70 (MQ=255)
       cAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTg                                                              <  1:2102441/71‑1 (MQ=255)
       cAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTg                                                              <  1:1979820/71‑1 (MQ=255)
       cAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTg                                                              <  1:2108013/71‑1 (MQ=255)
       cAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGACGGTGAGCTGATTTTCATTCTg                                                                  <  1:154939/67‑1 (MQ=255)
        aaaCCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGc                                                             <  1:1104878/71‑1 (MQ=255)
         aaCCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCt                                                            >  1:1807739/1‑71 (MQ=255)
              gTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCt                                                       >  1:2906340/1‑71 (MQ=255)
                ttCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGt                                                     >  1:3826078/1‑71 (MQ=255)
                  cACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGtt                                                    <  1:2248924/70‑1 (MQ=255)
                  cACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGtt                                                    <  1:4451963/70‑1 (MQ=255)
                     tCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCg                                                >  1:514956/1‑71 (MQ=255)
                     tCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCg                                                >  1:2635796/1‑71 (MQ=255)
                       ggTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTc                                                                    >  1:133127/1‑49 (MQ=255)
                       ggTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTc                                                                    >  1:3938101/1‑49 (MQ=255)
                       ggTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTc                                                                    >  1:985076/1‑49 (MQ=255)
                       ggTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGa                                                                 >  1:1956442/1‑52 (MQ=255)
                       ggTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCgtg                                              >  1:4747859/1‑71 (MQ=255)
                       ggTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCg                                                <  1:4102474/69‑1 (MQ=255)
                       ggTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCg                                                <  1:2739454/69‑1 (MQ=255)
                        gTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCgtgg                                             <  1:2549788/71‑1 (MQ=255)
                         cttGCGACTGTTCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCgtggt                                            >  1:3859644/2‑71 (MQ=255)
                         cttGCGACTGTTCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCgtggt                                            >  1:2293332/2‑71 (MQ=255)
                         cttGCGACTGTTCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCgtggt                                            >  1:3412248/2‑71 (MQ=255)
                         cttGCGACTGTTCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCgtggt                                            >  1:74925/2‑71 (MQ=255)
                             cGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTggtggt                                         <  1:696204/70‑1 (MQ=255)
                               aCTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTTGTCTGTTGCCGTGGTGGTcac                                      <  1:3958726/71‑1 (MQ=255)
                               aCTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTcac                                      <  1:1115985/71‑1 (MQ=255)
                               aCTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTcac                                      <  1:1789411/71‑1 (MQ=255)
                               aCTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTcac                                      <  1:3469622/71‑1 (MQ=255)
                               aCTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTcac                                      <  1:4201384/71‑1 (MQ=255)
                                   ttCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCGTGGTGGTCACAGt                                   <  1:2154619/70‑1 (MQ=255)
                                   ttCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCGTGGTGGTCACAGt                                   <  1:64827/70‑1 (MQ=255)
                                   ttCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGTTACCGTGGTGGTCACAGt                                   <  1:2488532/70‑1 (MQ=255)
                                   ttCGGTAACATGTATGCGGGTGAACTGATCTTTATCCTGATTGCGGGTCTGATACCGTGGTGGTCACAGt                                   <  1:1632785/70‑1 (MQ=255)
                                     cGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTgg                                 <  1:15451/70‑1 (MQ=255)
                                      ggTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGAt                               <  1:997892/71‑1 (MQ=255)
                                      ggTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGAt                               <  1:4606629/71‑1 (MQ=255)
                                      ggTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGAt                               >  1:2217735/1‑71 (MQ=255)
                                       gTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGa                                <  1:1384777/69‑1 (MQ=255)
                                         aaCATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAg                                    >  1:1712296/1‑63 (MQ=255)
                                          aCATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGAt                               <  1:743229/67‑1 (MQ=255)
                                           cATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTg                           >  1:256207/1‑70 (MQ=255)
                                           cATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTg                           >  1:3863304/1‑70 (MQ=255)
                                           cATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTg                           >  1:3447607/1‑70 (MQ=255)
                                           cATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTg                           >  1:3078560/1‑70 (MQ=255)
                                           cATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGa                          <  1:3911839/71‑1 (MQ=255)
                                           cATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGa                          <  1:2361874/71‑1 (MQ=255)
                                           cATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGa                          <  1:1962365/71‑1 (MQ=255)
                                             tGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTgg                                 >  1:4203597/1‑62 (MQ=255)
                                             tGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTgg                                 >  1:2334127/1‑62 (MQ=255)
                                               tATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAAtgt                      <  1:1362587/71‑1 (MQ=255)
                                               tATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAAtgt                      >  1:2402960/1‑71 (MQ=255)
                                                 tGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGc                    >  1:1662452/1‑71 (MQ=255)
                                                   ccGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGcc                   >  1:2085340/1‑70 (MQ=255)
                                                   ccGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCg                  <  1:1048145/71‑1 (MQ=255)
                                                   ccGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCg                  <  1:1042453/71‑1 (MQ=255)
                                                   ccGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCg                  >  1:3548770/1‑71 (MQ=255)
                                                     ggTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTg                >  1:2799251/1‑71 (MQ=255)
                                                     ggTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTCAATGTGCCGTg                >  1:3131664/1‑71 (MQ=255)
                                                     ggTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGGCACAGTGGATCCTGAATGTGCCGTg                <  1:4360089/71‑1 (MQ=255)
                                                      gTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTg                <  1:3695612/70‑1 (MQ=255)
                                                           cTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCAt          <  1:4393422/71‑1 (MQ=255)
                                                           cTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCAt          <  1:4398708/71‑1 (MQ=255)
                                                            tGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCAtt         >  1:4419638/1‑71 (MQ=255)
                                                             gATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCAttt        >  1:3552012/1‑71 (MQ=255)
                                                             gATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCAttt        >  1:972411/1‑71 (MQ=255)
                                                               ttttTATTCTGATTGCTGGTCTGTTGCCGTGGTGGTcaca                                     >  1:3003804/1‑40 (MQ=255)
                                                                tttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTggg              >  1:3011947/1‑62 (MQ=255)
                                                                tttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTggg              >  1:3163585/1‑62 (MQ=255)
                                                                tttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCAtttt       >  1:3544829/1‑69 (MQ=255)
                                                                tttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTc      >  1:446305/1‑70 (MQ=255)
                                                                tttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTc      >  1:1905292/1‑70 (MQ=255)
                                                                tttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTc      >  1:3592156/1‑70 (MQ=255)
                                                                 ttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAAt                        <  1:295231/51‑1 (MQ=255)
                                                                 ttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCAtttt       >  1:622970/1‑68 (MQ=255)
                                                                 ttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTcc     >  1:1887765/1‑70 (MQ=255)
                                                                 ttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTCca    >  1:686469/1‑71 (MQ=255)
                                                                 ttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTCca    >  1:2287400/1‑71 (MQ=255)
                                                                 ttCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTCca    >  1:1683319/1‑71 (MQ=255)
                                                                   cATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAAtgt                      >  1:2768369/1‑51 (MQ=255)
                                                                   cATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAAtgt                      >  1:2752542/1‑51 (MQ=255)
                                                                   cATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAAtgt                      >  1:994907/1‑51 (MQ=255)
                                                                    aTTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTCcaca  <  1:3039782/70‑1 (MQ=255)
                                                                      |                                                                   
TGCTGTCCAAACCAGTTTCACTCGGTTTGCGACTGTTCGGTAACATGTATGCCGGTGAGCTGATTTTCATTCTGATTGCTGGTCTGTTGCCGTGGTGGTCACAGTGGATCCTGAATGTGCCGTGGGCCATTTTCCACA  >  minE/2464941‑2465078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: