Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,469,978 G→C 5.6% V320V (GTG→GTC atpD → F1 sector of membrane‑bound ATP synthase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,469,9780GC 5.6% 344.0 / 7.8 106V320V (GTG→GTCatpDF1 sector of membrane‑bound ATP synthase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (54/46);  new base C (3/3);  total (57/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 114 total aligned reads displayed.

ATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGT  >  minE/2469909‑2470047
                                                                     |                                                                     
ataCGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTgg                                                                      >  1:3767022/1‑71 (MQ=255)
 taCGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTgg                                                                      <  1:3106008/70‑1 (MQ=255)
 taCGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTgg                                                                      >  1:849751/1‑70 (MQ=255)
 taCGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTgg                                                                      >  1:2542183/1‑70 (MQ=255)
 taCGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTgg                                                                      <  1:182821/70‑1 (MQ=255)
 taCGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTgg                                                                      >  1:1411898/1‑70 (MQ=255)
 taCGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGt                                                                     <  1:4399372/71‑1 (MQ=255)
  aCGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTa                                                                    >  1:35067/1‑71 (MQ=255)
   cGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTa                                                                    <  1:1592144/70‑1 (MQ=255)
           cGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGt                                                           >  1:909841/1‑71 (MQ=255)
             gATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGt                                                           >  1:1188678/1‑69 (MQ=255)
             gATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCa                                                         <  1:3408123/71‑1 (MQ=255)
              aTGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGt                                                           >  1:4318262/1‑68 (MQ=255)
              aTGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAg                                                        <  1:3786272/71‑1 (MQ=255)
              aTGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAg                                                        <  1:2933339/71‑1 (MQ=255)
              aTGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAg                                                        <  1:4146390/71‑1 (MQ=255)
              aTGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAg                                                        <  1:4550302/71‑1 (MQ=255)
              aTGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAg                                                        <  1:419823/71‑1 (MQ=255)
               tgacttgactGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAg                                                        <  1:3659978/70‑1 (MQ=255)
                gacttggctGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGt                                                           >  1:607228/1‑66 (MQ=255)
                  cttgactGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATcg                                                    >  1:4001699/1‑71 (MQ=255)
                   ttgactGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTg                                                                       >  1:3431600/1‑51 (MQ=255)
                   ttgactGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATcgc                                                   >  1:3997982/1‑71 (MQ=255)
                       ctgacCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGtct                                               <  1:3506926/71‑1 (MQ=255)
                          acCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTctct                                             <  1:85322/70‑1 (MQ=255)
                          acCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTctct                                             <  1:1752367/70‑1 (MQ=255)
                          acCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTctct                                             <  1:1611013/70‑1 (MQ=255)
                          acCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTctct                                             <  1:2921162/70‑1 (MQ=255)
                          acCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTg                                            >  1:2792014/1‑71 (MQ=255)
                          acCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTg                                            >  1:3232864/1‑71 (MQ=255)
                             cGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGt                                         <  1:2862459/71‑1 (MQ=255)
                             cGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGt                                         <  1:3589691/71‑1 (MQ=255)
                             cGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGt                                         <  1:908250/71‑1 (MQ=255)
                              gTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTa                                        <  1:1819097/71‑1 (MQ=255)
                                 tcCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTAt                                       >  1:3825130/1‑69 (MQ=255)
                                 tcCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATc                                      >  1:4016342/1‑70 (MQ=255)
                                   cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGAt                                                      >  1:3128941/1‑52 (MQ=255)
                                   cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGAt                                                      >  1:2130285/1‑52 (MQ=255)
                                   cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATcgc                                                   >  1:376330/1‑55 (MQ=255)
                                   cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTa                                    <  1:3438039/70‑1 (MQ=255)
                                   cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTa                                    <  1:150178/70‑1 (MQ=255)
                                   cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTa                                    <  1:869574/70‑1 (MQ=255)
                                    ggCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTa                                    <  1:244764/69‑1 (MQ=255)
                                     gCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTg                                            <  1:3428217/60‑1 (MQ=255)
                                     gCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTg                                            <  1:4256067/60‑1 (MQ=255)
                                     gCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTAccc                                 <  1:105299/71‑1 (MQ=255)
                                     gCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTAccc                                 <  1:4720782/71‑1 (MQ=255)
                                      cAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCg                                <  1:3161384/71‑1 (MQ=255)
                                      cAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCg                                <  1:4189578/71‑1 (MQ=255)
                                       aaCCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGCTCGCGTCTCTGGGTATCTACCCgg                               >  1:2563542/1‑71 (MQ=255)
                                       aaCCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCgg                               >  1:4063829/1‑71 (MQ=255)
                                          caccTTTGCGCACCTTGACGGAACCGTGGTACTGAGCCGTCAGATcg                                                    >  1:206391/1‑47 (MQ=255)
                                          caccTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCg                            >  1:2829053/1‑71 (MQ=255)
                                          caccTTCGCCCACCTGGACTCGACCGTCGTTCTGTCGCGTGACATCGCATCGCTGGGTATCTACCCTGcgg                            >  1:3415904/1‑69 (MQ=255)
                                          caccTTCGCCCACCTGGACTCGACCGTCGTTCTGTCGCGTGACATCGCATCGCTGGGTATCTACCCTGcgg                            >  1:2501833/1‑69 (MQ=255)
                                          caccTTCGCCCACCTGGACTCGACCGTCGTTCTGTCGCGTGACATCGCATCGCTGGGTATCTACCCTGcgg                            >  1:4025323/1‑69 (MQ=255)
                                           accTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGt                                                           >  1:463845/1‑39 (MQ=255)
                                           accTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGt                                                           >  1:1557602/1‑39 (MQ=255)
                                           accTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTAt                                       >  1:2342622/1‑59 (MQ=255)
                                           accTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCTGGCCGt                           <  1:1108/71‑1 (MQ=255)
                                           accTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGt                           <  1:4736167/71‑1 (MQ=255)
                                            ccTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGtt                          <  1:3585632/71‑1 (MQ=255)
                                            ccTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGtt                          >  1:1017555/1‑71 (MQ=255)
                                            ccTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGtt                          <  1:2324941/71‑1 (MQ=255)
                                            ccTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTATGGGTATCTACCCGGCCGtt                          <  1:1707410/71‑1 (MQ=255)
                                             cTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTa                                    >  1:3475372/1‑60 (MQ=255)
                                             cTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTAc                                   >  1:2893327/1‑61 (MQ=255)
                                             cTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTAc                                   >  1:426069/1‑61 (MQ=255)
                                             cTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATATAc                                   >  1:1254073/1‑61 (MQ=255)
                                               ttGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGt                                                 >  1:511006/1‑45 (MQ=255)
                                               ttGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGt                                                 >  1:1676958/1‑45 (MQ=255)
                                               ttGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGt                                                 >  1:25921/1‑45 (MQ=255)
                                               ttGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAc                       >  1:3774609/1‑71 (MQ=255)
                                               ttGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAc                       >  1:2247333/1‑71 (MQ=255)
                                                tGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCt                                     >  1:1432523/1‑56 (MQ=255)
                                                tGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAcc                      >  1:2835828/1‑71 (MQ=255)
                                                tGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAcc                      >  1:3236522/1‑71 (MQ=255)
                                                 gcgcACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAcc                      <  1:2577269/70‑1 (MQ=255)
                                                 gcgcACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAcc                      <  1:728405/70‑1 (MQ=255)
                                                  cgcACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAccc                     <  1:3554573/70‑1 (MQ=255)
                                                   ccACCTGGACGCGACCGTCGTACTGTCCCGTGACATCGCCTCGCTGGGTATCTACCCCGCGGTCGACCCGc                   <  1:759270/70‑1 (MQ=255)
                                                    cACCTGGACGCGACCGTCGTACTGTCCCGTGACATCGCCTCGCTGGGTATCTACCCCGCGGTCGACCCGc                   <  1:83372/70‑1 (MQ=255)
                                                     aCCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTg                 >  1:360219/1‑71 (MQ=255)
                                                     aCCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTg                 >  1:3383060/1‑71 (MQ=255)
                                                        ttGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCg                    >  1:253635/1‑65 (MQ=255)
                                                        ttGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCg                    >  1:1244383/1‑65 (MQ=255)
                                                        ttGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCg                    >  1:684147/1‑65 (MQ=255)
                                                        ttGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCg                    >  1:1802068/1‑65 (MQ=255)
                                                         tGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTa                                        >  1:2251103/1‑44 (MQ=255)
                                                         tGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTa                                        >  1:1885760/1‑44 (MQ=255)
                                                         tGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACt             >  1:3280172/1‑71 (MQ=255)
                                                         tGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACt             >  1:1984961/1‑71 (MQ=255)
                                                           aCGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTcc           <  1:4084956/71‑1 (MQ=255)
                                                            cGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTg                 <  1:1570183/64‑1 (MQ=255)
                                                              cAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTccac         >  1:395314/1‑70 (MQ=255)
                                                               aaCCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTccac         >  1:1038244/1‑69 (MQ=255)
                                                                aCCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAg      <  1:3378230/71‑1 (MQ=255)
                                                                aCCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAg      <  1:2985224/71‑1 (MQ=255)
                                                                 ccGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGc     >  1:1132770/1‑71 (MQ=255)
                                                                    tGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGt  >  1:3083266/1‑71 (MQ=255)
                                                                     |                                                                     
ATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGT  >  minE/2469909‑2470047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: