Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,469,993 G→C 5.6% Q325H (CAG→CAC) ‡ atpD → F1 sector of membrane‑bound ATP synthase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,469,9930GC 5.6% 352.7 / 6.7 107Q325H (CAG→CAC) ‡atpDF1 sector of membrane‑bound ATP synthase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (52/49);  new base C (3/3);  total (55/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 115 total aligned reads displayed.

ATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGACCCGCTG  >  minE/2469923‑2470062
                                                                      |                                                                     
aTGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACTCAACCGTGGTACTGAGCCGTCAg                                                                       <  1:165944/71‑1 (MQ=255)
aTGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAg                                                                       <  1:2933339/71‑1 (MQ=255)
aTGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAg                                                                       <  1:419823/71‑1 (MQ=255)
aTGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAg                                                                       <  1:3786272/71‑1 (MQ=255)
aTGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAg                                                                       <  1:132014/71‑1 (MQ=255)
aTGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAg                                                                       <  1:4550302/71‑1 (MQ=255)
 tgacttgactGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAg                                                                       <  1:3659978/70‑1 (MQ=255)
    cttgactGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATcg                                                                   >  1:4001699/1‑71 (MQ=255)
     ttgactGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATcgc                                                                  >  1:3997982/1‑71 (MQ=255)
         ctgacCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGtct                                                              <  1:3506926/71‑1 (MQ=255)
            acCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTctct                                                            <  1:1752367/70‑1 (MQ=255)
            acCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTctct                                                            <  1:85322/70‑1 (MQ=255)
            acCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTctct                                                            <  1:2921162/70‑1 (MQ=255)
            acCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTctct                                                            <  1:1611013/70‑1 (MQ=255)
            acCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTg                                                           >  1:2792014/1‑71 (MQ=255)
            acCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTg                                                           >  1:3232864/1‑71 (MQ=255)
               cGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGt                                                        <  1:3589691/71‑1 (MQ=255)
                gTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTa                                                       <  1:1819097/71‑1 (MQ=255)
                   tcCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTAt                                                      >  1:3825130/1‑69 (MQ=255)
                   tcCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATc                                                     >  1:4189031/1‑70 (MQ=255)
                   tcCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATc                                                     >  1:4016342/1‑70 (MQ=255)
                     cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGAt                                                                     >  1:2130285/1‑52 (MQ=255)
                     cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGAt                                                                     >  1:3128941/1‑52 (MQ=255)
                     cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATcgc                                                                  >  1:4562971/1‑55 (MQ=255)
                     cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATcgc                                                                  >  1:376330/1‑55 (MQ=255)
                     cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTa                                                   <  1:2598282/70‑1 (MQ=255)
                     cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTa                                                   <  1:150178/70‑1 (MQ=255)
                     cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTa                                                   <  1:3438039/70‑1 (MQ=255)
                     cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTa                                                   <  1:869574/70‑1 (MQ=255)
                      ggCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTa                                                   <  1:244764/69‑1 (MQ=255)
                       gCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTg                                                           <  1:3428217/60‑1 (MQ=255)
                       gCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTg                                                           <  1:4256067/60‑1 (MQ=255)
                       gCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTAccc                                                <  1:4720782/71‑1 (MQ=255)
                       gCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTAccc                                                <  1:105299/71‑1 (MQ=255)
                        cAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCg                                               <  1:4189578/71‑1 (MQ=255)
                        cAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCg                                               <  1:3161384/71‑1 (MQ=255)
                         aaCCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGCTCGCGTCTCTGGGTATCTACCCgg                                              >  1:2563542/1‑71 (MQ=255)
                         aaCCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCgg                                              >  1:4063829/1‑71 (MQ=255)
                            caccTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCg                                           >  1:735537/1‑71 (MQ=255)
                            caccTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCg                                           >  1:2829053/1‑71 (MQ=255)
                            caccTTCGCCCACCTGGACTCGACCGTCGTTCTGTCGCGTGACATCGCATCGCTGGGTATCTACCCTGcgg                                           >  1:4025323/1‑69 (MQ=255)
                            caccTTCGCCCACCTGGACTCGACCGTCGTTCTGTCGCGTGACATCGCATCGCTGGGTATCTACCCTGcgg                                           >  1:2501833/1‑69 (MQ=255)
                             accTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTAt                                                      >  1:2342622/1‑59 (MQ=255)
                             accTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCTGGCCGt                                          <  1:1108/71‑1 (MQ=255)
                             accTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGt                                          <  1:4736167/71‑1 (MQ=255)
                              ccTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGtt                                         <  1:3585632/71‑1 (MQ=255)
                              ccTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGtt                                         >  1:1017555/1‑71 (MQ=255)
                              ccTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGtt                                         <  1:2324941/71‑1 (MQ=255)
                               cTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTa                                                   >  1:3475372/1‑60 (MQ=255)
                               cTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTAc                                                  >  1:2893327/1‑61 (MQ=255)
                               cTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTAc                                                  >  1:426069/1‑61 (MQ=255)
                               cTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATATAc                                                  >  1:1254073/1‑61 (MQ=255)
                                 ttGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGt                                                                >  1:1676958/1‑45 (MQ=255)
                                 ttGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGt                                                                >  1:25921/1‑45 (MQ=255)
                                 ttGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGt                                                                >  1:511006/1‑45 (MQ=255)
                                 ttGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAc                                      >  1:3774609/1‑71 (MQ=255)
                                 ttGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAc                                      >  1:2247333/1‑71 (MQ=255)
                                  tGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCt                                                    >  1:1432523/1‑56 (MQ=255)
                                  tGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAcc                                     >  1:2835828/1‑71 (MQ=255)
                                   gcgcACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAcc                                     <  1:2577269/70‑1 (MQ=255)
                                    cgcACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAccc                                    <  1:3554573/70‑1 (MQ=255)
                                     ccACCTGGACGCGACCGTCGTACTGTCCCGTGACATCGCCTCGCTGGGTATCTACCCCGCGGTCGACCCGc                                  <  1:759270/70‑1 (MQ=255)
                                      cACCTGGACGCGACCGTCGTACTGTCCCGTGACATCGCCTCGCTGGGTATCTACCCCGCGGTCGACCCGc                                  <  1:83372/70‑1 (MQ=255)
                                       aCCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTctct                                                            <  1:3237960/43‑1 (MQ=255)
                                       aCCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTg                                <  1:2977417/71‑1 (MQ=255)
                                       aCCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTg                                >  1:3383060/1‑71 (MQ=255)
                                        ccTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCt                                 >  1:1975031/1‑69 (MQ=255)
                                          ttGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCg                                   >  1:684147/1‑65 (MQ=255)
                                          ttGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCg                                   >  1:253635/1‑65 (MQ=255)
                                          ttGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCg                                   >  1:1244383/1‑65 (MQ=255)
                                          ttGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCg                                   >  1:1802068/1‑65 (MQ=255)
                                           tGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTa                                                       >  1:2251103/1‑44 (MQ=255)
                                           tGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTa                                                       >  1:1885760/1‑44 (MQ=255)
                                           tGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACt                            >  1:1984961/1‑71 (MQ=255)
                                           tGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACt                            >  1:3280172/1‑71 (MQ=255)
                                             aCGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTcc                          <  1:4084956/71‑1 (MQ=255)
                                              cGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTg                                <  1:1570183/64‑1 (MQ=255)
                                                cAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTccac                        >  1:395314/1‑70 (MQ=255)
                                                 aaCCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTccac                        >  1:1038244/1‑69 (MQ=255)
                                                  aCCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAg                     <  1:3378230/71‑1 (MQ=255)
                                                   ccGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGc                    >  1:1132770/1‑71 (MQ=255)
                                                      tGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGt                 >  1:3083266/1‑71 (MQ=255)
                                                        gTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGt                 >  1:225749/1‑69 (MQ=255)
                                                        gTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTc                <  1:3932433/70‑1 (MQ=255)
                                                        gTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTc                <  1:2704942/70‑1 (MQ=255)
                                                        gTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTc                <  1:4330114/70‑1 (MQ=255)
                                                        gTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTc                <  1:2988030/70‑1 (MQ=255)
                                                         tACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTc                <  1:2963858/69‑1 (MQ=255)
                                                         tACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAg              <  1:3365512/71‑1 (MQ=255)
                                                            tGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCTGCCGTTGACCCGCt                                 >  1:3114619/1‑49 (MQ=255)
                                                            tGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCt                                 >  1:3048460/1‑49 (MQ=255)
                                                            tGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCt                                 >  1:2881687/1‑49 (MQ=255)
                                                            tGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCt                                 >  1:2733773/1‑49 (MQ=255)
                                                                 cGTCAGATCGCGTCTCTGGGTATCTACCCGTCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGAccc      <  1:2908460/71‑1 (MQ=255)
                                                                  gTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGACCCg     <  1:4619698/71‑1 (MQ=255)
                                                                  gTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGACCCg     <  1:783580/71‑1 (MQ=255)
                                                                   tCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAcc                                     <  1:4328833/38‑1 (MQ=255)
                                                                    cAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGACCCGCt   >  1:2304354/1‑71 (MQ=255)
                                                                     aGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGACCCGCtg  <  1:2736971/71‑1 (MQ=255)
                                                                     aGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGACCCGCtg  <  1:3252841/71‑1 (MQ=255)
                                                                      |                                                                     
ATGACTTGACTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGACCCGCTG  >  minE/2469923‑2470062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: