Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,470,002 T→G 5.8% S328S (TCT→TCG atpD → F1 sector of membrane‑bound ATP synthase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,470,0020TG 5.8% 284.7 / 12.5 103S328S (TCT→TCGatpDF1 sector of membrane‑bound ATP synthase, beta subunit
Reads supporting (aligned to +/- strand):  ref base T (50/47);  new base G (3/3);  total (53/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01

Only 100 of 113 total aligned reads displayed.

CTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGACCCGCTGGTGGTTGG  >  minE/2469932‑2470070
                                                                      |                                                                    
ctgacCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGtct                                                                      <  1:3506926/71‑1 (MQ=255)
   acCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTctct                                                                    <  1:2921162/70‑1 (MQ=255)
   acCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTctct                                                                    <  1:85322/70‑1 (MQ=255)
   acCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTctct                                                                    <  1:1611013/70‑1 (MQ=255)
   acCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTg                                                                   >  1:3232864/1‑71 (MQ=255)
   acCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTg                                                                   >  1:2792014/1‑71 (MQ=255)
      cGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGt                                                                <  1:3589691/71‑1 (MQ=255)
      cGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGt                                                                <  1:908250/71‑1 (MQ=255)
      cGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGt                                                                <  1:2862459/71‑1 (MQ=255)
       gTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTa                                                               <  1:1819097/71‑1 (MQ=255)
          tcCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTAt                                                              >  1:3825130/1‑69 (MQ=255)
          tcCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATc                                                             >  1:4189031/1‑70 (MQ=255)
            cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTa                                                           <  1:2598282/70‑1 (MQ=255)
            cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTa                                                           <  1:3438039/70‑1 (MQ=255)
            cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTa                                                           <  1:150178/70‑1 (MQ=255)
            cGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTa                                                           <  1:869574/70‑1 (MQ=255)
             ggCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTa                                                           <  1:244764/69‑1 (MQ=255)
              gCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTg                                                                   <  1:4256067/60‑1 (MQ=255)
              gCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTg                                                                   <  1:3428217/60‑1 (MQ=255)
              gCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTAccc                                                        <  1:4720782/71‑1 (MQ=255)
              gCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTAccc                                                        <  1:105299/71‑1 (MQ=255)
               cAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCg                                                       <  1:3161384/71‑1 (MQ=255)
               cAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCg                                                       <  1:4189578/71‑1 (MQ=255)
                aaCCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCgg                                                      >  1:4063829/1‑71 (MQ=255)
                   caccTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCg                                                   >  1:735537/1‑71 (MQ=255)
                   caccTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCg                                                   >  1:2829053/1‑71 (MQ=255)
                   caccTTCGCCCACCTGGACTCGACCGTCGTTCTGTCGCGTGACATCGCATCGCTGGGTATCTACCCTGcgg                                                   >  1:3415904/1‑69 (MQ=255)
                   caccTTCGCCCACCTGGACTCGACCGTCGTTCTGTCGCGTGACATCGCATCGCTGGGTATCTACCCTGcgg                                                   >  1:4025323/1‑69 (MQ=255)
                   caccTTCGCCCACCTGGACTCGACCGTCGTTCTGTCGCGTGACATCGCATCGCTGGGTATCTACCCTGcgg                                                   >  1:2501833/1‑69 (MQ=255)
                    accTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTAt                                                              >  1:2342622/1‑59 (MQ=255)
                    accTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCTGGCCGt                                                  <  1:1108/71‑1 (MQ=255)
                    accTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGt                                                  <  1:4736167/71‑1 (MQ=255)
                     ccTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGtt                                                 <  1:2324941/71‑1 (MQ=255)
                     ccTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGtt                                                 <  1:3585632/71‑1 (MQ=255)
                     ccTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGtt                                                 >  1:1017555/1‑71 (MQ=255)
                     ccTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTATGGGTATCTACCCGGCCGtt                                                 <  1:1707410/71‑1 (MQ=255)
                      cTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTa                                                           >  1:3475372/1‑60 (MQ=255)
                      cTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTAc                                                          >  1:426069/1‑61 (MQ=255)
                      cTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTAc                                                          >  1:2893327/1‑61 (MQ=255)
                      cTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTg                                                <  1:2410584/71‑1 (MQ=255)
                      cTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATATAc                                                          >  1:1254073/1‑61 (MQ=255)
                        ttGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAc                                              >  1:3774609/1‑71 (MQ=255)
                        ttGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAc                                              >  1:2247333/1‑71 (MQ=255)
                         tGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCt                                                            >  1:1432523/1‑56 (MQ=255)
                         tGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAcc                                             >  1:3236522/1‑71 (MQ=255)
                          gcgcACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAcc                                             <  1:2577269/70‑1 (MQ=255)
                           cgcACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAccc                                            <  1:3554573/70‑1 (MQ=255)
                            ccACCTGGACGCGACCGTCGTACTGTCCCGTGACATCGCCTCGCTGGGTATCTACCCCGCGGTCGACCCGc                                          <  1:759270/70‑1 (MQ=255)
                            ccACCTGGACGCGACCGTCGTACTGTCCCGTGACATCGCCTCGCTGGGTATCTACCCCGCGGTCGACCCGc                                          <  1:3967805/70‑1 (MQ=255)
                             cACCTGGACGCGACCGTCGTACTGTCCCGTGACATCGCCTCGCTGGGTATCTACCCCGCGGTCGACCCGc                                          <  1:83372/70‑1 (MQ=255)
                              aCCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTg                                        <  1:2977417/71‑1 (MQ=255)
                              aCCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTg                                        >  1:3383060/1‑71 (MQ=255)
                               ccTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCt                                         >  1:1975031/1‑69 (MQ=255)
                                 ttGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCg                                           >  1:684147/1‑65 (MQ=255)
                                 ttGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCg                                           >  1:1244383/1‑65 (MQ=255)
                                 ttGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCg                                           >  1:253635/1‑65 (MQ=255)
                                 ttGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCg                                           >  1:1802068/1‑65 (MQ=255)
                                  tGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTa                                                               >  1:2251103/1‑44 (MQ=255)
                                  tGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTa                                                               >  1:1885760/1‑44 (MQ=255)
                                  tGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACt                                    >  1:3280172/1‑71 (MQ=255)
                                  tGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACt                                    >  1:1984961/1‑71 (MQ=255)
                                    aCGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTcc                                  <  1:4084956/71‑1 (MQ=255)
                                     cGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTg                                        <  1:1570183/64‑1 (MQ=255)
                                       cAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTccac                                >  1:395314/1‑70 (MQ=255)
                                        aaCCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTccac                                >  1:1038244/1‑69 (MQ=255)
                                         aCCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAg                             <  1:2985224/71‑1 (MQ=255)
                                         aCCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAg                             <  1:3378230/71‑1 (MQ=255)
                                          ccGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGc                            >  1:1132770/1‑71 (MQ=255)
                                             tGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGt                         >  1:3083266/1‑71 (MQ=255)
                                               gTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGt                         >  1:225749/1‑69 (MQ=255)
                                               gTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTc                        <  1:4330114/70‑1 (MQ=255)
                                               gTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTc                        <  1:2704942/70‑1 (MQ=255)
                                               gTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTc                        <  1:3932433/70‑1 (MQ=255)
                                                tACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTc                        <  1:2963858/69‑1 (MQ=255)
                                                tACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAg                      <  1:3365512/71‑1 (MQ=255)
                                                   tGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCTGCCGTTGACCCGCt                                         >  1:3114619/1‑49 (MQ=255)
                                                   tGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCt                                         >  1:3048460/1‑49 (MQ=255)
                                                   tGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCt                                         >  1:2881687/1‑49 (MQ=255)
                                                   tGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCt                                         >  1:2733773/1‑49 (MQ=255)
                                                     aGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGa                 <  1:2621914/71‑1 (MQ=255)
                                                        cGTCAGATCGCGTCTCTGGGTATCTACCCGTCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGAccc              <  1:2908460/71‑1 (MQ=255)
                                                         gTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGACCCg             <  1:783580/71‑1 (MQ=255)
                                                         gTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGACCCg             <  1:4619698/71‑1 (MQ=255)
                                                          tCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGAcc                                             <  1:4328833/38‑1 (MQ=255)
                                                           cAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGACCCGCt           >  1:2304354/1‑71 (MQ=255)
                                                            aGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGACCCGCtg          <  1:4358972/71‑1 (MQ=255)
                                                            aGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGACCCGCtg          <  1:2736971/71‑1 (MQ=255)
                                                            aGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGACCCGCtg          <  1:3252841/71‑1 (MQ=255)
                                                                cgcgTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGACCCGCtggtgg      >  1:42642/1‑71 (MQ=255)
                                                                cgcgTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGACCCGCtggtgg      >  1:2999206/1‑71 (MQ=255)
                                                                cgcgTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGACCCGCtggtgg      >  1:1464905/1‑71 (MQ=255)
                                                                cgcgTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGACCCGCtggtgg      >  1:3394059/1‑71 (MQ=255)
                                                                cgcgTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGACCCGCtggtg       >  1:357913/1‑70 (MQ=255)
                                                                 gcgTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGACCCGCtggtgg      <  1:3537378/70‑1 (MQ=255)
                                                                  cgTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGc                            >  1:3700213/1‑47 (MQ=255)
                                                                  cgTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGACCCGCTGGtggtt    >  1:166475/1‑71 (MQ=255)
                                                                    tctcTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGAcc               >  1:4387830/1‑58 (MQ=255)
                                                                    tctcTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGACCCGCTGGtggttgg  >  1:3713279/1‑71 (MQ=255)
                                                                    tctcTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGACCCGCTGGtggttgg  >  1:1268334/1‑71 (MQ=255)
                                                                      tctGGGTATCTACCCGGCCGTTGACCCGCTGGACTc                                   <  1:1354343/36‑1 (MQ=255)
                                                                      |                                                                    
CTGACCCGTCTCCGGCAACCACCTTTGCGCACCTTGACGCAACCGTGGTACTGAGCCGTCAGATCGCGTCTCTGGGTATCTACCCGGCCGTTGACCCGCTGGACTCCACCAGCCGTCAGCTGGACCCGCTGGTGGTTGG  >  minE/2469932‑2470070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: