| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,546,001 | C→T | 12.2% | M182I (ATG→ATA) | rfaF ← | ADP‑heptose:LPS heptosyltransferase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,546,001 | 0 | C | T | 12.2% | 209.6 / 17.9 | 74 | M182I (ATG→ATA) | rfaF | ADP‑heptose:LPS heptosyltransferase II |
| Reads supporting (aligned to +/- strand): ref base C (35/30); new base T (7/2); total (42/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
CAGCTCCGCATAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCTCACCTGC > minE/2545932‑2546067 | cAGCTCCGCATAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAatca < 1:3359410/71‑1 (MQ=255)cAGCTCCGCATAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAatca < 1:594248/71‑1 (MQ=255) aGCTCCGCATAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAatcat > 1:1609315/1‑71 (MQ=255) cTCCGCATAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCg < 1:2330424/71‑1 (MQ=255) ccGCATAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCgg > 1:533611/1‑70 (MQ=255) cATAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGt > 1:3582739/1‑71 (MQ=255) aTAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGtt < 1:868464/71‑1 (MQ=255) tAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAatcat > 1:4151609/1‑62 (MQ=255) tAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAatcat > 1:4603892/1‑62 (MQ=255) aGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCt > 1:4712654/1‑71 (MQ=255) gTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTc > 1:405645/1‑69 (MQ=255) gTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCt < 1:1492670/70‑1 (MQ=255) gTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCt < 1:4429108/70‑1 (MQ=255) gTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACg > 1:230831/1‑63 (MQ=255) gTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCtgat > 1:162103/1‑70 (MQ=255) tAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCtgatga < 1:3602246/71‑1 (MQ=255) tAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCtgatga < 1:3319189/71‑1 (MQ=255) tAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCtgatga < 1:1769439/71‑1 (MQ=255) tAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCtgatga < 1:4106470/71‑1 (MQ=255) tAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCtgatga < 1:4430246/71‑1 (MQ=255) tgtgGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAg < 1:1592098/71‑1 (MQ=255) tgtgGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAg < 1:3212634/71‑1 (MQ=255) tgtgGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAg < 1:2533582/71‑1 (MQ=255) tgGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCtg > 1:3563363/1‑62 (MQ=255) ccAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCg < 1:641559/50‑1 (MQ=255) cAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGaaaa > 1:3390226/1‑71 (MQ=255) gCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCg < 1:1297638/47‑1 (MQ=255) gCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCt < 1:877915/55‑1 (MQ=255) gCGTTTTGCCGGACCAAACTCCGCACCCCGGCAAAAACCAATCATCg < 1:4525573/47‑1 (MQ=255) cGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAAtt > 1:1605816/1‑70 (MQ=255) cGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAAtt > 1:3919048/1‑70 (MQ=255) gTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGa < 1:2161640/71‑1 (MQ=255) ttttGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCtgat < 1:1349342/55‑1 (MQ=255) ttttGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGAt < 1:3017572/71‑1 (MQ=255) tttGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCtgat < 1:314869/54‑1 (MQ=255) ttGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGAtt < 1:1701226/70‑1 (MQ=255) ttGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGAtt < 1:1051582/70‑1 (MQ=255) ttGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTa > 1:3261103/1‑71 (MQ=255) ttGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTa > 1:4513071/1‑71 (MQ=255) ttGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTa > 1:1424445/1‑71 (MQ=255) cGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGaaa > 1:3067947/1‑53 (MQ=255) cGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGaaa > 1:3720002/1‑53 (MQ=255) ggACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTACAgg > 1:4667334/1‑70 (MQ=255) ggACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAgg > 1:3539872/1‑70 (MQ=255) ggACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAgg > 1:1047925/1‑70 (MQ=255) gACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGta > 1:3468954/1‑71 (MQ=255) aCCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGAt > 1:2802629/1‑62 (MQ=255) ccAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGaaa > 1:2934664/1‑55 (MQ=255) ccAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGaaa > 1:4343775/1‑55 (MQ=255) ccAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGaaa > 1:4306301/1‑55 (MQ=255) aaCTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGaa < 1:2418039/51‑1 (MQ=255) aaCTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGaa < 1:1255756/51‑1 (MQ=255) aaCTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGaa < 1:3030242/51‑1 (MQ=255) aaCTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGa > 1:2355657/1‑71 (MQ=255) aaCTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGa > 1:1293649/1‑71 (MQ=255) aaCTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGa > 1:4344409/1‑71 (MQ=255) aCTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGAt > 1:735788/1‑71 (MQ=255) tCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGAttt > 1:1787920/1‑71 (MQ=255) gggCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTAc > 1:3726017/1‑49 (MQ=255) gggCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTAc > 1:3115075/1‑49 (MQ=255) gggCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTAc > 1:3426067/1‑49 (MQ=255) gggCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTAc > 1:1663846/1‑49 (MQ=255) gggCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTAc > 1:4077453/1‑49 (MQ=255) ggCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCtt < 1:2074303/69‑1 (MQ=255) ggCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCtt < 1:3915304/69‑1 (MQ=255) aaaaaCCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCTc < 1:2235067/71‑1 (MQ=255) aaaaCCAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCTc < 1:151967/70‑1 (MQ=255) aaaaCCAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCTCa < 1:1758869/71‑1 (MQ=255) aaaaCCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCTCa > 1:3824965/1‑71 (MQ=255) aaaCCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCTCAc > 1:1692164/1‑71 (MQ=255) aaaCCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCTCAc > 1:3268759/1‑71 (MQ=255) aaCCAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCTCAc > 1:1690063/1‑70 (MQ=255) aaCCAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCTCAc > 1:1082002/1‑70 (MQ=255) aaCCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCTCa > 1:2635578/1‑69 (MQ=255) aaCCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCTCAcc > 1:387121/1‑71 (MQ=255) aaCCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCTCAcc > 1:2115633/1‑71 (MQ=255) aaCCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCTCAcc > 1:827814/1‑71 (MQ=255) cAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCTCACCTGc < 1:3385324/71‑1 (MQ=255) cAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCTCACCTGc < 1:1754168/71‑1 (MQ=255) | CAGCTCCGCATAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCTCACCTGC > minE/2545932‑2546067 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |