| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,550,360 | A→G | 5.2% | K77K (AAA→AAG) | tdh → | threonine 3‑dehydrogenase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,550,360 | 0 | A | G | 5.2% | 355.6 / 8.1 | 115 | K77K (AAA→AAG) | tdh | threonine 3‑dehydrogenase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base A (46/63); new base G (2/4); total (48/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01 | |||||||||||
Only 100 of 131 total aligned reads displayed.
CCATCCCGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCGTG > minE/2550290‑2550430 | ccATCCCGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGaaa < 1:682186/71‑1 (MQ=255) cATCCCGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGaaa < 1:3125199/70‑1 (MQ=255) tCCCGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGc > 1:801471/1‑71 (MQ=255) tCCCGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGc > 1:528096/1‑71 (MQ=255) tCCCGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGc > 1:4342717/1‑71 (MQ=255) ccGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCtt < 1:1642766/71‑1 (MQ=255) ccGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCt < 1:3003499/70‑1 (MQ=255) cGGTGCCGATGGTCGTGTGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTc < 1:2977184/71‑1 (MQ=255) ccGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTc > 1:3066377/1‑66 (MQ=255) ccGATGGTCGT‑GGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGAt < 1:2151049/70‑1 (MQ=255) aTGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCgg > 1:957461/1‑71 (MQ=255) aTGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCgg > 1:1364808/1‑71 (MQ=255) tGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGc > 1:4652203/1‑71 (MQ=255) ggTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGc > 1:3393361/1‑70 (MQ=255) gTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGa < 1:2646126/71‑1 (MQ=255) tCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGAt > 1:2743746/1‑64 (MQ=255) tCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGAt > 1:1942026/1‑64 (MQ=255) cGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGAt > 1:306313/1‑70 (MQ=255) cGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATc > 1:2263092/1‑71 (MQ=255) cGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATc > 1:2595138/1‑71 (MQ=255) gTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGTAAGTGAAAGGCTTCAAGATCGGCGATcg < 1:2116906/71‑1 (MQ=255) gTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATcg > 1:2823804/1‑71 (MQ=255) gTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATcg < 1:2956457/71‑1 (MQ=255) gTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATcg < 1:2966847/71‑1 (MQ=255) gTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATcg < 1:1671984/71‑1 (MQ=255) gTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATcg < 1:516544/71‑1 (MQ=255) gTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATcg < 1:3342940/71‑1 (MQ=255) cATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCaa > 1:4102270/1‑53 (MQ=255) cATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCaa > 1:1153003/1‑53 (MQ=255) cATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTc < 1:4536235/71‑1 (MQ=255) cATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTc < 1:4385435/71‑1 (MQ=255) cATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTc < 1:4608995/71‑1 (MQ=255) cATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTc < 1:667324/71‑1 (MQ=255) cATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTc < 1:1995717/71‑1 (MQ=255) cATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTc < 1:2854795/71‑1 (MQ=255) aTGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTc < 1:1152654/70‑1 (MQ=255) tGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTg > 1:2715460/1‑71 (MQ=255) gAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGCTCGCGTTTCTg > 1:3272736/1‑70 (MQ=255) gAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGAt < 1:1577877/60‑1 (MQ=255) gAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGAt < 1:2482900/60‑1 (MQ=255) aaTATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGaaa > 1:3111811/1‑41 (MQ=255) atatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGAt > 1:3099506/1‑58 (MQ=255) atatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGAt > 1:2637789/1‑58 (MQ=255) atatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCg < 1:3303431/71‑1 (MQ=255) atatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCg < 1:1767327/71‑1 (MQ=255) atatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCg < 1:3309076/71‑1 (MQ=255) atatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCg < 1:2596376/71‑1 (MQ=255) tatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCg < 1:2751996/70‑1 (MQ=255) tatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCg < 1:1967012/70‑1 (MQ=255) tGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGaa < 1:4587990/70‑1 (MQ=255) gTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCaa < 1:3247171/44‑1 (MQ=255) gTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCaa < 1:3298511/44‑1 (MQ=255) gTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCaa < 1:1856269/44‑1 (MQ=255) gTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCaa < 1:2593733/44‑1 (MQ=255) tCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGc < 1:4322422/71‑1 (MQ=255) tCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGc < 1:1762859/71‑1 (MQ=255) tGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCata > 1:4508543/1‑71 (MQ=255) tGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCg < 1:4196183/42‑1 (MQ=255) tGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCg < 1:4199082/42‑1 (MQ=255) gtaggtaTTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCtg < 1:4513393/71‑1 (MQ=255) taggtaTTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCg < 1:3277762/54‑1 (MQ=255) ggtaTTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTgg > 1:135233/1‑71 (MQ=255) ggtaTTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTgg > 1:3695607/1‑71 (MQ=255) ggtaTTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGACATATCACCTGTgg > 1:2704223/1‑71 (MQ=255) gtaTTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGt < 1:2853615/71‑1 (MQ=255) taTTGGTCAGGTAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATc < 1:3922620/61‑1 (MQ=255) taTTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATc < 1:1275630/61‑1 (MQ=255) taTTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATc < 1:2437388/61‑1 (MQ=255) ttGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAgg < 1:860678/52‑1 (MQ=255) ttGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCa > 1:1980668/1‑60 (MQ=255) ttGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCAc > 1:2414054/1‑61 (MQ=255) ttGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCAc > 1:3555846/1‑61 (MQ=255) ttGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCAc > 1:1570136/1‑61 (MQ=255) ttGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCAt > 1:2218610/1‑71 (MQ=255) ttGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCAt > 1:2621222/1‑71 (MQ=255) tGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCt < 1:4638172/43‑1 (MQ=255) tGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCt < 1:2344619/43‑1 (MQ=255) tGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCt < 1:1620687/43‑1 (MQ=255) ggTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCAt > 1:3794158/1‑69 (MQ=255) gTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTg > 1:1655344/1‑70 (MQ=255) cAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCg > 1:3954261/1‑71 (MQ=255) cAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCg > 1:4259778/1‑71 (MQ=255) cAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCg > 1:3515754/1‑71 (MQ=255) aGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCt < 1:4714567/38‑1 (MQ=255) aGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCt < 1:2348375/38‑1 (MQ=255) aGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCt < 1:4246565/38‑1 (MQ=255) aGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCg > 1:201027/1‑70 (MQ=255) aGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGc < 1:3535518/71‑1 (MQ=255) aGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGc < 1:121590/71‑1 (MQ=255) aGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGc < 1:108448/71‑1 (MQ=255) aGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACt > 1:4440097/1‑71 (MQ=255) aGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACt < 1:3819946/71‑1 (MQ=255) aaaGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCg < 1:4298120/71‑1 (MQ=255) aaaGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCg > 1:3363864/1‑71 (MQ=255) aaGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCg < 1:235979/70‑1 (MQ=255) aGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCgtg > 1:2353570/1‑71 (MQ=255) aGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCgtg > 1:4282902/1‑71 (MQ=255) aGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCgtg > 1:3247909/1‑71 (MQ=255) aGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCgtg < 1:66885/71‑1 (MQ=255) aGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCgtg > 1:1758254/1‑71 (MQ=255) | CCATCCCGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCGTG > minE/2550290‑2550430 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |