| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,589,325 | A→G | 16.0% | intergenic (‑1/+798) | gadE ← / ← hdeD | DNA‑binding transcriptional activator/acid‑resistance membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,589,325 | 0 | A | G | 16.0% | 302.7 / 45.5 | 119 | intergenic (‑1/+798) | gadE/hdeD | DNA‑binding transcriptional activator/acid‑resistance membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (60/40); new base G (16/3); total (76/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.63e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
Only 100 of 121 total aligned reads displayed.
TCGTGATTATCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCGAATCGT > minE/2589257‑2589395 | tCGTGATTATCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAAc > 1:2241166/1‑71 (MQ=255)tCGTGATTATCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAAc > 1:2598225/1‑71 (MQ=255) cGTGATTATCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATGAc < 1:1821156/70‑1 (MQ=255) cGTGATTATCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACt > 1:65058/1‑71 (MQ=255) cGTGATTATCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACt > 1:4002113/1‑71 (MQ=255) ttATCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTc > 1:4249509/1‑71 (MQ=255) ttATCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTc > 1:3742350/1‑71 (MQ=255) aTCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGc > 1:1768673/1‑67 (MQ=255) aTCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCt > 1:1099925/1‑71 (MQ=255) tCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCt > 1:4519097/1‑70 (MQ=255) tCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCtt < 1:3315743/67‑1 (MQ=255) tCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGcc > 1:673607/1‑71 (MQ=255) tCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGcc > 1:1394879/1‑71 (MQ=255) tCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGcc > 1:3460820/1‑71 (MQ=255) aaCTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGt < 1:3951234/71‑1 (MQ=255) aaCTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGt < 1:3817865/71‑1 (MQ=255) aCTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGt < 1:841832/70‑1 (MQ=255) cAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGt > 1:1191877/1‑71 (MQ=255) aaaaGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATGACTTGCTCCTTAGCCGTTATCGtt < 1:4264437/71‑1 (MQ=255) aGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTc > 1:476914/1‑70 (MQ=255) aGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTc > 1:2559597/1‑70 (MQ=255) aGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTc > 1:435992/1‑70 (MQ=255) cccTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGaa < 1:1070963/71‑1 (MQ=255) ccTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAat < 1:4629090/71‑1 (MQ=255) ccTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAat < 1:4071160/71‑1 (MQ=255) cTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGaa < 1:207882/69‑1 (MQ=255) gTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGt < 1:2813413/55‑1 (MQ=255) gTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGt < 1:2591457/55‑1 (MQ=255) tAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATGACTTGCTCCTTAGCCGTTATCGTTTCGAATATg < 1:1721525/70‑1 (MQ=255) tAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACt > 1:505798/1‑40 (MQ=255) tAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGtt < 1:3537784/55‑1 (MQ=255) tAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGtt < 1:2151354/55‑1 (MQ=255) tAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGaa > 1:1832402/1‑66 (MQ=255) tAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGaa > 1:133893/1‑66 (MQ=255) tAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAatat < 1:193688/69‑1 (MQ=255) tAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAatat < 1:2396186/69‑1 (MQ=255) aaaagaaaagAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCt > 1:786134/1‑46 (MQ=255) aaaagaaaagAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCg > 1:3671317/1‑52 (MQ=255) aaaagaaaagAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAatat < 1:3266966/68‑1 (MQ=255) aaagaaaagaATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCg > 1:569840/1‑51 (MQ=255) aaagaaaagaATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCg > 1:4641000/1‑51 (MQ=255) aaagaaaagaATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCa > 1:3737949/1‑71 (MQ=255) aaagaaaagaATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCa > 1:1140254/1‑71 (MQ=255) aaagaaaagaATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCa > 1:3651576/1‑71 (MQ=255) aaagaaaagaATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCa > 1:299929/1‑71 (MQ=255) aaagaaaagaATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCa > 1:3743323/1‑71 (MQ=255) aagaaaagaaTCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCg < 1:3882483/56‑1 (MQ=255) agaaaagaaTCTTTCGTCATGAGAAAAATCATAACTTGCt > 1:1719082/1‑40 (MQ=255) agaaaagaaTCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAata > 1:1830804/1‑64 (MQ=255) agaaaagaaTCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAata > 1:1413552/1‑64 (MQ=255) agaaaagaaTCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATc < 1:1477385/71‑1 (MQ=255) aaagaaTCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCtt < 1:1511895/71‑1 (MQ=255) aagaaTCTTTCGTCATGAGAAAAATCATGACTTGCTCCTTAGGCGTTATCGttt > 1:293767/1‑54 (MQ=255) aagaaTCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCttt > 1:665273/1‑71 (MQ=255) aagaaTCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCttt > 1:2174603/1‑71 (MQ=255) aaTCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCg < 1:4466108/53‑1 (MQ=255) aTCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCat > 1:1690084/1‑71 (MQ=255) aTCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCat < 1:2283152/71‑1 (MQ=255) aTCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCat > 1:3599007/1‑71 (MQ=255) tttCGTCATGAGAAAAATCATGACTTGCTCCTTAGCCGTTa > 1:4001482/1‑41 (MQ=255) tttCGTCATGAGAAAAATCATGACTTGCTCCTTAGCCGTTa > 1:882981/1‑41 (MQ=255) tttCGTCATGAGAAAAATCATGACTTGCTCCTTAGCCGTTa > 1:3268399/1‑41 (MQ=255) tttCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGaa < 1:80760/51‑1 (MQ=255) tttCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCtttt < 1:2144644/65‑1 (MQ=255) tttCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCatta > 1:4269030/1‑70 (MQ=255) tttCGTAATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCatta > 1:4762768/1‑70 (MQ=255) ttCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCg < 1:3556116/37‑1 (MQ=255) tCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATc > 1:1212899/1‑58 (MQ=255) tCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCatta < 1:48057/68‑1 (MQ=255) tCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCatta < 1:3335921/68‑1 (MQ=255) tCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCatta < 1:4309165/68‑1 (MQ=255) cGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATc > 1:2810527/1‑57 (MQ=255) aTGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATc > 1:1750738/1‑71 (MQ=255) gagaAAAATCATGACTTGCTCCTTAGCCGTTATCGTTTCGAata > 1:651938/1‑44 (MQ=255) gagaAAAATCATGACTTGCTCCTTAGCCGTTATCGTTTCGAata > 1:691947/1‑44 (MQ=255) gagaAAAATCATGACTTGCTCCTTAGCCGTTATCGTTTCGAata > 1:4700605/1‑44 (MQ=255) gagaAAAATCATGACTTGCTCCTTAGCCGTTATCGTTTCGAata > 1:1999319/1‑44 (MQ=255) gagaAAAATCATGACTTGCTCCTTAGCCGTTATCGTTTCGAata > 1:1332746/1‑44 (MQ=255) gagaAAAATCATGACTTGCTCCTTAGCCGTTATCGTTTCGAata > 1:2032167/1‑44 (MQ=255) gagaAAAATCATGACTTGCTCCTTAGCCGTTATCGTTTCGAata > 1:4340860/1‑44 (MQ=255) gagaAAAATCATGACTTGCTCCTTAGCCGTTATCGTTTCGAata > 1:3321657/1‑44 (MQ=255) gagaAAAATCATGACTTGCTCCTTAGCCGTTATCGTTTCGAata > 1:4139348/1‑44 (MQ=255) gagaAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCattat > 1:1810308/1‑62 (MQ=255) gaAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTg > 1:3241235/1‑71 (MQ=255) aaaTCATAACTTGCTCCTTAGCCGTTATCGTTTCGAata > 1:4625579/1‑39 (MQ=255) aaaTCATAACTTGCTCCTTAGCCGTTATCGTTTCGAata > 1:2704462/1‑39 (MQ=255) aaaTCATAACTTGCTCCTTAGCCGTTATCGTTTCGAata > 1:139028/1‑39 (MQ=255) aaaTCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTcc > 1:1602844/1‑71 (MQ=255) tCATAACTTGCTCCTTAGCCGTTATCGTTTCGAatat < 1:339916/37‑1 (MQ=255) tCATAACTTGCTCCTTAGCCGTTATCGTTTCGAatat < 1:2024410/37‑1 (MQ=255) tCATAACTTGCTCCTTAGCCGTTATCGTTTCGAatat < 1:3020044/37‑1 (MQ=255) tCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCGaa > 1:1964900/1‑71 (MQ=255) tCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCGaa > 1:3129989/1‑71 (MQ=255) tCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCGaa > 1:1719268/1‑71 (MQ=255) tCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCGaa > 1:3094167/1‑71 (MQ=255) tCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCGaa < 1:2092745/71‑1 (MQ=255) aTAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGt > 1:3470094/1‑37 (MQ=255) aTAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCGAATc > 1:1517834/1‑71 (MQ=255) aaCTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCGAATCgt > 1:1885792/1‑71 (MQ=255) aaCTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCGAATCgt > 1:2233255/1‑71 (MQ=255) | TCGTGATTATCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCGAATCGT > minE/2589257‑2589395 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |