| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,625,149 | G→A | 14.3% | R16H (CGT→CAT) | ftsX → | predicted transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,625,149 | 0 | G | A | 14.3% | 149.7 / 15.4 | 56 | R16H (CGT→CAT) | ftsX | predicted transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (26/22); new base A (2/6); total (28/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
CACTTGCATGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGTAACGCACCAAAACG > minE/2625081‑2625212 | cACTTGCATGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGt > 1:978401/1‑70 (MQ=255) aCTTGCATGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCt > 1:4343981/1‑71 (MQ=255) gCATGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTg < 1:3183533/69‑1 (MQ=255) gCATGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTg < 1:4594076/69‑1 (MQ=255) gCATGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTg < 1:4488780/69‑1 (MQ=255) gCATGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTg < 1:4339193/69‑1 (MQ=255) gCATGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTg < 1:2537370/69‑1 (MQ=255) gCATGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGAt > 1:1282369/1‑71 (MQ=255) gCATGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGAt > 1:1332022/1‑71 (MQ=255) aTGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATc > 1:4346075/1‑70 (MQ=255) gAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCt > 1:650619/1‑62 (MQ=255) ggCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTcc > 1:1659923/1‑71 (MQ=255) gCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTcc < 1:50498/70‑1 (MQ=255) gTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGt > 1:3642010/1‑70 (MQ=255) gggCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTaaa > 1:1080442/1‑71 (MQ=255) gggCCATGAATAAGCGCGATGCAATAAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCg > 1:4426267/1‑67 (MQ=255) tGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTc < 1:4063123/48‑1 (MQ=255) tGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAAtcggtc > 1:4225322/1‑71 (MQ=255) tGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAAtcggt > 1:4544801/1‑70 (MQ=255) aaTAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCtt > 1:695010/1‑48 (MQ=255) aaTAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGAt > 1:2232247/1‑51 (MQ=255) aaTAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGAt > 1:1893321/1‑51 (MQ=255) aaTAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGAt > 1:831051/1‑51 (MQ=255) aTAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTcggc < 1:405395/71‑1 (MQ=255) tAAGCGCGATGCAATCAATCATATTCGGCAGTTTggcgggcg < 1:2629866/42‑1 (MQ=255) aGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTc > 1:2054804/1‑42 (MQ=255) aGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTc > 1:1344516/1‑42 (MQ=255) aGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCggcggc > 1:4150845/1‑71 (MQ=255) aGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCggcggc > 1:1189390/1‑71 (MQ=255) gcgcGATGCAATCAATCATATTCAGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCt < 1:1956514/71‑1 (MQ=255) gcgATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCa < 1:2968556/71‑1 (MQ=255) caatcaatCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAc < 1:4405058/71‑1 (MQ=255) caatcaatCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAc < 1:3235697/71‑1 (MQ=255) caatcaatCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAc < 1:1707400/71‑1 (MQ=255) caatcaatCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAc < 1:3861646/71‑1 (MQ=255) caatcaatCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAc < 1:1417346/71‑1 (MQ=255) aatcaatcATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAcg < 1:141899/71‑1 (MQ=255) atcaatcaTATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAAt < 1:1510537/49‑1 (MQ=255) atcaatcaTATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAcgg < 1:1300679/71‑1 (MQ=255) atcaatcaTATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAcgg > 1:3482416/1‑71 (MQ=255) atatTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGt < 1:4046702/71‑1 (MQ=255) tatTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAAt < 1:1361080/41‑1 (MQ=255) tatTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGt > 1:2110119/1‑70 (MQ=255) tatTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGt > 1:2734930/1‑70 (MQ=255) tatTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTaa < 1:708199/39‑1 (MQ=255) tatTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTaa < 1:22238/39‑1 (MQ=255) tatTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTaa < 1:2645296/39‑1 (MQ=255) ttCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAAtcg < 1:2512835/41‑1 (MQ=255) ttCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGTCGGCGGTCGTaa > 1:3626656/1‑70 (MQ=255) ttCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGTaa > 1:4499019/1‑70 (MQ=255) tCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAAtcg < 1:1309142/40‑1 (MQ=255) cGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGTAACg < 1:2528308/70‑1 (MQ=255) tttGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCg < 1:2856683/49‑1 (MQ=255) tttGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCg < 1:1536876/49‑1 (MQ=255) ttGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGTAACGCACCaaaa > 1:4526598/1‑71 (MQ=255) ttGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGTAACGCACCaaaa > 1:4361211/1‑71 (MQ=255) tGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGTAACGCACCaaaa > 1:1338447/1‑70 (MQ=255) gcgggcgTCTTGATCGCTTCCGTAAATCGGTCGACGGCTCAGGCGACGGCGGTCGTAACGCACCAAAAcg < 1:3435355/70‑1 (MQ=255) | CACTTGCATGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGTAACGCACCAAAACG > minE/2625081‑2625212 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |